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Diffusion Tensor Imaging in Rat Models of Preclinical Diabetic Nephropathy: A Preliminary Study

PURPOSE: This study aimed to investigate the value of diffusion tensor imaging to assess renal injury in a rat model of preclinical diabetic nephropathy. METHODS: Twenty-eight male Sprague Dawley rats were divided into two groups: the normal control (NC) group of 10 rats and the diabetic nephropathy...

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Autores principales: Hu, Xiaoyan, Kuang, Min, Peng, Bo, Yang, Yang, Lin, Wei, Li, Wenbo, Wu, Yinghua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8429902/
https://www.ncbi.nlm.nih.gov/pubmed/34512547
http://dx.doi.org/10.3389/fendo.2021.701116
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author Hu, Xiaoyan
Kuang, Min
Peng, Bo
Yang, Yang
Lin, Wei
Li, Wenbo
Wu, Yinghua
author_facet Hu, Xiaoyan
Kuang, Min
Peng, Bo
Yang, Yang
Lin, Wei
Li, Wenbo
Wu, Yinghua
author_sort Hu, Xiaoyan
collection PubMed
description PURPOSE: This study aimed to investigate the value of diffusion tensor imaging to assess renal injury in a rat model of preclinical diabetic nephropathy. METHODS: Twenty-eight male Sprague Dawley rats were divided into two groups: the normal control (NC) group of 10 rats and the diabetic nephropathy (DN) group of 18 rats. Eight weeks after diabetes induction by streptozotocin, 3.0-T magnetic resonance (MR) imaging (b = 0 and 600 s/mm(2), 15 diffusion directions) using a 32-channel knee coil was performed. After MR imaging, we measured serum creatinine, and collected double kidney tissues for pathology. The apparent diffusion coefficients(ADC) and fractional anisotropy(FA) values of the renal cortex and medulla were calculated for all kidneys. Physiological parameters, laboratory parameters, and imaging results were compared between the two groups. RESULTS: All DN group animals developed hyperglycemia, polyuria, and emaciation. Serum creatinine was not significantly different between the groups (P > 0.05). Urinary albumin at 2, 4, and 8 weeks was higher in the DN group than in the NC group but <20 µg/min (P < 0.05). Pathologically, renal damage in the DN rats was observed. The ADC value was significantly increased in DN animals in the cortex (1.75×10(-3)mm(2)/s),medulla(1.53×10(-3)mm(2)/s)compared with NC group(cortex, 1.52×10(-3)mm(2)/s; medulla,1.35×10(-3)mm(2)/s). The FA value was significantly reduced in DN animals in the cortex (0.21),medulla(0.25)compared with NC group(cortex,0.26;medulla,0.3). CONCLUSIONS: Increased apparent diffusion coefficients and decreased fractional anisotropy values on diffusion tensor imaging were associated with preclinical DN. Diffusion tensor imaging may be useful in early, non-invasive, quantitative detection, and therapy monitoring of DN.
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spelling pubmed-84299022021-09-11 Diffusion Tensor Imaging in Rat Models of Preclinical Diabetic Nephropathy: A Preliminary Study Hu, Xiaoyan Kuang, Min Peng, Bo Yang, Yang Lin, Wei Li, Wenbo Wu, Yinghua Front Endocrinol (Lausanne) Endocrinology PURPOSE: This study aimed to investigate the value of diffusion tensor imaging to assess renal injury in a rat model of preclinical diabetic nephropathy. METHODS: Twenty-eight male Sprague Dawley rats were divided into two groups: the normal control (NC) group of 10 rats and the diabetic nephropathy (DN) group of 18 rats. Eight weeks after diabetes induction by streptozotocin, 3.0-T magnetic resonance (MR) imaging (b = 0 and 600 s/mm(2), 15 diffusion directions) using a 32-channel knee coil was performed. After MR imaging, we measured serum creatinine, and collected double kidney tissues for pathology. The apparent diffusion coefficients(ADC) and fractional anisotropy(FA) values of the renal cortex and medulla were calculated for all kidneys. Physiological parameters, laboratory parameters, and imaging results were compared between the two groups. RESULTS: All DN group animals developed hyperglycemia, polyuria, and emaciation. Serum creatinine was not significantly different between the groups (P > 0.05). Urinary albumin at 2, 4, and 8 weeks was higher in the DN group than in the NC group but <20 µg/min (P < 0.05). Pathologically, renal damage in the DN rats was observed. The ADC value was significantly increased in DN animals in the cortex (1.75×10(-3)mm(2)/s),medulla(1.53×10(-3)mm(2)/s)compared with NC group(cortex, 1.52×10(-3)mm(2)/s; medulla,1.35×10(-3)mm(2)/s). The FA value was significantly reduced in DN animals in the cortex (0.21),medulla(0.25)compared with NC group(cortex,0.26;medulla,0.3). CONCLUSIONS: Increased apparent diffusion coefficients and decreased fractional anisotropy values on diffusion tensor imaging were associated with preclinical DN. Diffusion tensor imaging may be useful in early, non-invasive, quantitative detection, and therapy monitoring of DN. Frontiers Media S.A. 2021-08-27 /pmc/articles/PMC8429902/ /pubmed/34512547 http://dx.doi.org/10.3389/fendo.2021.701116 Text en Copyright © 2021 Hu, Kuang, Peng, Yang, Lin, Li and Wu https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Endocrinology
Hu, Xiaoyan
Kuang, Min
Peng, Bo
Yang, Yang
Lin, Wei
Li, Wenbo
Wu, Yinghua
Diffusion Tensor Imaging in Rat Models of Preclinical Diabetic Nephropathy: A Preliminary Study
title Diffusion Tensor Imaging in Rat Models of Preclinical Diabetic Nephropathy: A Preliminary Study
title_full Diffusion Tensor Imaging in Rat Models of Preclinical Diabetic Nephropathy: A Preliminary Study
title_fullStr Diffusion Tensor Imaging in Rat Models of Preclinical Diabetic Nephropathy: A Preliminary Study
title_full_unstemmed Diffusion Tensor Imaging in Rat Models of Preclinical Diabetic Nephropathy: A Preliminary Study
title_short Diffusion Tensor Imaging in Rat Models of Preclinical Diabetic Nephropathy: A Preliminary Study
title_sort diffusion tensor imaging in rat models of preclinical diabetic nephropathy: a preliminary study
topic Endocrinology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8429902/
https://www.ncbi.nlm.nih.gov/pubmed/34512547
http://dx.doi.org/10.3389/fendo.2021.701116
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