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Silencing of PFKFB3 protects podocytes against high glucose-induced injury by inducing autophagy
Diabetic nephropathy (DN) is a diabetic complication that threatens the health of patients with diabetes. In addition, podocyte injury can lead to the occurrence of DN. The protein 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (PFKFB3) may be associated with diabetes; however, the effects of...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8430303/ https://www.ncbi.nlm.nih.gov/pubmed/34490476 http://dx.doi.org/10.3892/mmr.2021.12405 |
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author | Zhu, Zhengming Liu, Qingsheng Sun, Jianshi Bao, Ziyang Wang, Weiwei |
author_facet | Zhu, Zhengming Liu, Qingsheng Sun, Jianshi Bao, Ziyang Wang, Weiwei |
author_sort | Zhu, Zhengming |
collection | PubMed |
description | Diabetic nephropathy (DN) is a diabetic complication that threatens the health of patients with diabetes. In addition, podocyte injury can lead to the occurrence of DN. The protein 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (PFKFB3) may be associated with diabetes; however, the effects of PFKFB3 knockdown by small interfering (si)RNA on the growth of podocytes remains unknown. To investigate the mechanism by which PFKFB3 mediates podocyte injury, MPC5 mouse podocyte cells were treated with high-glucose (HG), and cell viability and apoptosis were examined by Cell Counting Kit-8 assay and flow cytometry, respectively. In addition, the expression of autophagy-related proteins were measured using western blot analysis and immunofluorescence staining. Cell migration was investigated using a Transwell assay and phalloidin staining was performed to observe the cytoskeleton. The results revealed that silencing of PFKFB3 significantly promoted MPC5 cell viability and inhibited apoptosis. In addition, the migration of the MPC5 cells was notably downregulated by siPFKFB3. Moreover, PFKFB3 silencing notably reversed the HG-induced decrease in oxygen consumption rate, and the HG-induced increase in extracellular acidification rate was rescued by PFKFB3 siRNA. Furthermore, silencing of PFKFB3 induced autophagy in HG-treated podocytes through inactivating phosphorylated (p-)mTOR, p-AMPKα, LC3 and sirtuin 1, and activating p62. In conclusion, silencing of PFKFB3 may protect podocytes from HG-induced injury by inducing autophagy. Therefore, PFKFB3 may serve as a potential target for treatment of DN. |
format | Online Article Text |
id | pubmed-8430303 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-84303032021-09-23 Silencing of PFKFB3 protects podocytes against high glucose-induced injury by inducing autophagy Zhu, Zhengming Liu, Qingsheng Sun, Jianshi Bao, Ziyang Wang, Weiwei Mol Med Rep Articles Diabetic nephropathy (DN) is a diabetic complication that threatens the health of patients with diabetes. In addition, podocyte injury can lead to the occurrence of DN. The protein 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (PFKFB3) may be associated with diabetes; however, the effects of PFKFB3 knockdown by small interfering (si)RNA on the growth of podocytes remains unknown. To investigate the mechanism by which PFKFB3 mediates podocyte injury, MPC5 mouse podocyte cells were treated with high-glucose (HG), and cell viability and apoptosis were examined by Cell Counting Kit-8 assay and flow cytometry, respectively. In addition, the expression of autophagy-related proteins were measured using western blot analysis and immunofluorescence staining. Cell migration was investigated using a Transwell assay and phalloidin staining was performed to observe the cytoskeleton. The results revealed that silencing of PFKFB3 significantly promoted MPC5 cell viability and inhibited apoptosis. In addition, the migration of the MPC5 cells was notably downregulated by siPFKFB3. Moreover, PFKFB3 silencing notably reversed the HG-induced decrease in oxygen consumption rate, and the HG-induced increase in extracellular acidification rate was rescued by PFKFB3 siRNA. Furthermore, silencing of PFKFB3 induced autophagy in HG-treated podocytes through inactivating phosphorylated (p-)mTOR, p-AMPKα, LC3 and sirtuin 1, and activating p62. In conclusion, silencing of PFKFB3 may protect podocytes from HG-induced injury by inducing autophagy. Therefore, PFKFB3 may serve as a potential target for treatment of DN. D.A. Spandidos 2021-11 2021-09-03 /pmc/articles/PMC8430303/ /pubmed/34490476 http://dx.doi.org/10.3892/mmr.2021.12405 Text en Copyright: © Zhu et al. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Zhu, Zhengming Liu, Qingsheng Sun, Jianshi Bao, Ziyang Wang, Weiwei Silencing of PFKFB3 protects podocytes against high glucose-induced injury by inducing autophagy |
title | Silencing of PFKFB3 protects podocytes against high glucose-induced injury by inducing autophagy |
title_full | Silencing of PFKFB3 protects podocytes against high glucose-induced injury by inducing autophagy |
title_fullStr | Silencing of PFKFB3 protects podocytes against high glucose-induced injury by inducing autophagy |
title_full_unstemmed | Silencing of PFKFB3 protects podocytes against high glucose-induced injury by inducing autophagy |
title_short | Silencing of PFKFB3 protects podocytes against high glucose-induced injury by inducing autophagy |
title_sort | silencing of pfkfb3 protects podocytes against high glucose-induced injury by inducing autophagy |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8430303/ https://www.ncbi.nlm.nih.gov/pubmed/34490476 http://dx.doi.org/10.3892/mmr.2021.12405 |
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