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Infants Younger Than 90 Days Admitted for Late-Onset Sepsis Display a Reduced Abundance of Regulatory T Cells
OBJECTIVE: To provide epidemiological data of infants < 90 days of age with suspected late-onset sepsis (LOS) and evaluate distinct immunological specificities. We hypothesized that previously healthy infants < 3 months of age with sepsis have a yet undefined immunological predisposition; e.g....
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8430331/ https://www.ncbi.nlm.nih.gov/pubmed/34512621 http://dx.doi.org/10.3389/fimmu.2021.666447 |
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author | Fortmann, Ingmar Dammann, Marie-Theres Siller, Bastian Humberg, Alexander Demmert, Martin Tüshaus, Ludger Lindert, Judith van Zandbergen, Vera Pagel, Julia Rupp, Jan Herting, Egbert Härtel, Christoph |
author_facet | Fortmann, Ingmar Dammann, Marie-Theres Siller, Bastian Humberg, Alexander Demmert, Martin Tüshaus, Ludger Lindert, Judith van Zandbergen, Vera Pagel, Julia Rupp, Jan Herting, Egbert Härtel, Christoph |
author_sort | Fortmann, Ingmar |
collection | PubMed |
description | OBJECTIVE: To provide epidemiological data of infants < 90 days of age with suspected late-onset sepsis (LOS) and evaluate distinct immunological specificities. We hypothesized that previously healthy infants < 3 months of age with sepsis have a yet undefined immunological predisposition; e.g. differences in lymphocyte subsets including regulatory T cells. METHODS: We performed an exploratory, single center study between January 1(st), 2019 and June 1(st), 2021. Routine diagnostics included conventional culture (blood, cerebrospinal fluid, urine), PCR and inflammatory markers in infants < 90 days of age with suspected sepsis. We additionally analyzed lymphocyte subsets and CD4+ CD25+ forkhead box protein (FoxP3)(+) Tregs at admission for sepsis workup as compared to age-matched controls. RESULTS: A convenience sample cohort of n= 51 infants with sepsis workup was enrolled. Invasive bacterial infection (IBI) was diagnosed in 25 (49.0%) patients including two infants with a rhinovirus co-infection and viral infection in 14 (27.5%) neonates. No infectious cause was found in 12 cases. Infants with suspected LOS displayed a decreased abundance of CD4+ FoxP3+ T cells as compared to controls, which was most pronounced in the subgroup of infants with IBI. We also noticed elevated HLA-DR-positive CD3+ cells in infants with LOS and a higher CD4/CD8-ratio in infants with viral infection as compared to healthy controls. Infants with viral infections had a higher number of natural killer cells as compared to infants with IBI. CONCLUSION: Our exploratory data support the concept of a potential immaturity state and failed immune tolerance development for young infants with LOS. Future large-scale studies are needed to elucidate pre-sepsis conditions and to target the microbiome-immunity interplay as a potential risk pattern. |
format | Online Article Text |
id | pubmed-8430331 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-84303312021-09-11 Infants Younger Than 90 Days Admitted for Late-Onset Sepsis Display a Reduced Abundance of Regulatory T Cells Fortmann, Ingmar Dammann, Marie-Theres Siller, Bastian Humberg, Alexander Demmert, Martin Tüshaus, Ludger Lindert, Judith van Zandbergen, Vera Pagel, Julia Rupp, Jan Herting, Egbert Härtel, Christoph Front Immunol Immunology OBJECTIVE: To provide epidemiological data of infants < 90 days of age with suspected late-onset sepsis (LOS) and evaluate distinct immunological specificities. We hypothesized that previously healthy infants < 3 months of age with sepsis have a yet undefined immunological predisposition; e.g. differences in lymphocyte subsets including regulatory T cells. METHODS: We performed an exploratory, single center study between January 1(st), 2019 and June 1(st), 2021. Routine diagnostics included conventional culture (blood, cerebrospinal fluid, urine), PCR and inflammatory markers in infants < 90 days of age with suspected sepsis. We additionally analyzed lymphocyte subsets and CD4+ CD25+ forkhead box protein (FoxP3)(+) Tregs at admission for sepsis workup as compared to age-matched controls. RESULTS: A convenience sample cohort of n= 51 infants with sepsis workup was enrolled. Invasive bacterial infection (IBI) was diagnosed in 25 (49.0%) patients including two infants with a rhinovirus co-infection and viral infection in 14 (27.5%) neonates. No infectious cause was found in 12 cases. Infants with suspected LOS displayed a decreased abundance of CD4+ FoxP3+ T cells as compared to controls, which was most pronounced in the subgroup of infants with IBI. We also noticed elevated HLA-DR-positive CD3+ cells in infants with LOS and a higher CD4/CD8-ratio in infants with viral infection as compared to healthy controls. Infants with viral infections had a higher number of natural killer cells as compared to infants with IBI. CONCLUSION: Our exploratory data support the concept of a potential immaturity state and failed immune tolerance development for young infants with LOS. Future large-scale studies are needed to elucidate pre-sepsis conditions and to target the microbiome-immunity interplay as a potential risk pattern. Frontiers Media S.A. 2021-08-27 /pmc/articles/PMC8430331/ /pubmed/34512621 http://dx.doi.org/10.3389/fimmu.2021.666447 Text en Copyright © 2021 Fortmann, Dammann, Siller, Humberg, Demmert, Tüshaus, Lindert, van Zandbergen, Pagel, Rupp, Herting and Härtel https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Immunology Fortmann, Ingmar Dammann, Marie-Theres Siller, Bastian Humberg, Alexander Demmert, Martin Tüshaus, Ludger Lindert, Judith van Zandbergen, Vera Pagel, Julia Rupp, Jan Herting, Egbert Härtel, Christoph Infants Younger Than 90 Days Admitted for Late-Onset Sepsis Display a Reduced Abundance of Regulatory T Cells |
title | Infants Younger Than 90 Days Admitted for Late-Onset Sepsis Display a Reduced Abundance of Regulatory T Cells |
title_full | Infants Younger Than 90 Days Admitted for Late-Onset Sepsis Display a Reduced Abundance of Regulatory T Cells |
title_fullStr | Infants Younger Than 90 Days Admitted for Late-Onset Sepsis Display a Reduced Abundance of Regulatory T Cells |
title_full_unstemmed | Infants Younger Than 90 Days Admitted for Late-Onset Sepsis Display a Reduced Abundance of Regulatory T Cells |
title_short | Infants Younger Than 90 Days Admitted for Late-Onset Sepsis Display a Reduced Abundance of Regulatory T Cells |
title_sort | infants younger than 90 days admitted for late-onset sepsis display a reduced abundance of regulatory t cells |
topic | Immunology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8430331/ https://www.ncbi.nlm.nih.gov/pubmed/34512621 http://dx.doi.org/10.3389/fimmu.2021.666447 |
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