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Vitamin D3 Enriches Ceramide Content in Exosomes Released by Embryonic Hippocampal Cells
The release of exosomes can lead to cell–cell communication. Nutrients such as vitamin D3 and sphingolipids have important roles in many cellular functions, including proliferation, differentiation, senescence, and cancer. However, the specific composition of sphingolipids in exosomes and their chan...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8430480/ https://www.ncbi.nlm.nih.gov/pubmed/34502192 http://dx.doi.org/10.3390/ijms22179287 |
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author | Conte, Carmela Cataldi, Samuela Arcuri, Cataldo Mirarchi, Alessandra Lazzarini, Andrea Garcia-Gil, Mercedes Beccari, Tommaso Curcio, Francesco Albi, Elisabetta |
author_facet | Conte, Carmela Cataldi, Samuela Arcuri, Cataldo Mirarchi, Alessandra Lazzarini, Andrea Garcia-Gil, Mercedes Beccari, Tommaso Curcio, Francesco Albi, Elisabetta |
author_sort | Conte, Carmela |
collection | PubMed |
description | The release of exosomes can lead to cell–cell communication. Nutrients such as vitamin D3 and sphingolipids have important roles in many cellular functions, including proliferation, differentiation, senescence, and cancer. However, the specific composition of sphingolipids in exosomes and their changes induced by vitamin D3 treatment have not been elucidated. Here, we initially observed neutral sphingomyelinase and vitamin D receptors in exosomes released from HN9.10 embryonic hippocampal cells. Using ultrafast liquid chromatography tandem mass spectrometry, we showed that exosomes are rich in sphingomyelin species compared to whole cells. To interrogate the possible functions of vitamin D3, we established the optimal conditions of cell treatment and we analyzed exosome composition. Vitamin D3 was identified as responsible for the vitamin D receptor loss, for the increase in neutral sphingomyelinase content and sphingomyelin changes. As a consequence, the generation of ceramide upon vitamin D3 treatment was evident. Incubation of the cells with neutral sphingomyelinase, or the same concentration of ceramide produced in exosomes was necessary and sufficient to stimulate embryonic hippocampal cell differentiation, as vitamin D3. This is the first time that exosome ceramide is interrogated for mediate the effect of vitamin D3 in inducing cell differentiation. |
format | Online Article Text |
id | pubmed-8430480 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-84304802021-09-11 Vitamin D3 Enriches Ceramide Content in Exosomes Released by Embryonic Hippocampal Cells Conte, Carmela Cataldi, Samuela Arcuri, Cataldo Mirarchi, Alessandra Lazzarini, Andrea Garcia-Gil, Mercedes Beccari, Tommaso Curcio, Francesco Albi, Elisabetta Int J Mol Sci Article The release of exosomes can lead to cell–cell communication. Nutrients such as vitamin D3 and sphingolipids have important roles in many cellular functions, including proliferation, differentiation, senescence, and cancer. However, the specific composition of sphingolipids in exosomes and their changes induced by vitamin D3 treatment have not been elucidated. Here, we initially observed neutral sphingomyelinase and vitamin D receptors in exosomes released from HN9.10 embryonic hippocampal cells. Using ultrafast liquid chromatography tandem mass spectrometry, we showed that exosomes are rich in sphingomyelin species compared to whole cells. To interrogate the possible functions of vitamin D3, we established the optimal conditions of cell treatment and we analyzed exosome composition. Vitamin D3 was identified as responsible for the vitamin D receptor loss, for the increase in neutral sphingomyelinase content and sphingomyelin changes. As a consequence, the generation of ceramide upon vitamin D3 treatment was evident. Incubation of the cells with neutral sphingomyelinase, or the same concentration of ceramide produced in exosomes was necessary and sufficient to stimulate embryonic hippocampal cell differentiation, as vitamin D3. This is the first time that exosome ceramide is interrogated for mediate the effect of vitamin D3 in inducing cell differentiation. MDPI 2021-08-27 /pmc/articles/PMC8430480/ /pubmed/34502192 http://dx.doi.org/10.3390/ijms22179287 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Conte, Carmela Cataldi, Samuela Arcuri, Cataldo Mirarchi, Alessandra Lazzarini, Andrea Garcia-Gil, Mercedes Beccari, Tommaso Curcio, Francesco Albi, Elisabetta Vitamin D3 Enriches Ceramide Content in Exosomes Released by Embryonic Hippocampal Cells |
title | Vitamin D3 Enriches Ceramide Content in Exosomes Released by Embryonic Hippocampal Cells |
title_full | Vitamin D3 Enriches Ceramide Content in Exosomes Released by Embryonic Hippocampal Cells |
title_fullStr | Vitamin D3 Enriches Ceramide Content in Exosomes Released by Embryonic Hippocampal Cells |
title_full_unstemmed | Vitamin D3 Enriches Ceramide Content in Exosomes Released by Embryonic Hippocampal Cells |
title_short | Vitamin D3 Enriches Ceramide Content in Exosomes Released by Embryonic Hippocampal Cells |
title_sort | vitamin d3 enriches ceramide content in exosomes released by embryonic hippocampal cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8430480/ https://www.ncbi.nlm.nih.gov/pubmed/34502192 http://dx.doi.org/10.3390/ijms22179287 |
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