Cargando…
Genome-Wide Identification of ARF Transcription Factor Gene Family and Their Expression Analysis in Sweet Potato
Auxin response factors (ARFs) are a family of transcription factors that play an important role of auxin regulation through their binding with auxin response elements. ARF genes are represented by a large multigene family in plants; however, to our knowledge, the ARF gene family has not been well st...
Autores principales: | , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8431151/ https://www.ncbi.nlm.nih.gov/pubmed/34502298 http://dx.doi.org/10.3390/ijms22179391 |
_version_ | 1783750869693497344 |
---|---|
author | Pratt, Isaac Seth Zhang, Baohong |
author_facet | Pratt, Isaac Seth Zhang, Baohong |
author_sort | Pratt, Isaac Seth |
collection | PubMed |
description | Auxin response factors (ARFs) are a family of transcription factors that play an important role of auxin regulation through their binding with auxin response elements. ARF genes are represented by a large multigene family in plants; however, to our knowledge, the ARF gene family has not been well studied and characterized in sweet potatoes. In this study, a total of 25 ARF genes were identified in Ipomea trifida. The identified ItrARF genes’ conserved motifs, chromosomal locations, phylogenetic relationships, and their protein characteristics were systemically investigated using different bioinformatics tools. The expression patterns of ItfARF genes were analyzed within the storage roots and normal roots at an early stage of development. ItfARF16b and ItfARF16c were both highly expressed in the storage root, with minimal to no expression in the normal root. ItfARF6a and ItfARF10a exhibited higher expression in the normal root but not in the storage root. Subsequently, ItfARF1a, ItfARF2b, ItfARF3a, ItfARF6b, ItfARF8a, ItfARF8b, and ItfARF10b were expressed in both root types with moderate to high expression for each. All ten of these ARF genes and their prominent expression signify their importance within the development of each respective root type. This study provides comprehensive information regarding the ARF family in sweet potatoes, which will be useful for future research to discover further functional verification of these ItfARF genes. |
format | Online Article Text |
id | pubmed-8431151 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-84311512021-09-11 Genome-Wide Identification of ARF Transcription Factor Gene Family and Their Expression Analysis in Sweet Potato Pratt, Isaac Seth Zhang, Baohong Int J Mol Sci Article Auxin response factors (ARFs) are a family of transcription factors that play an important role of auxin regulation through their binding with auxin response elements. ARF genes are represented by a large multigene family in plants; however, to our knowledge, the ARF gene family has not been well studied and characterized in sweet potatoes. In this study, a total of 25 ARF genes were identified in Ipomea trifida. The identified ItrARF genes’ conserved motifs, chromosomal locations, phylogenetic relationships, and their protein characteristics were systemically investigated using different bioinformatics tools. The expression patterns of ItfARF genes were analyzed within the storage roots and normal roots at an early stage of development. ItfARF16b and ItfARF16c were both highly expressed in the storage root, with minimal to no expression in the normal root. ItfARF6a and ItfARF10a exhibited higher expression in the normal root but not in the storage root. Subsequently, ItfARF1a, ItfARF2b, ItfARF3a, ItfARF6b, ItfARF8a, ItfARF8b, and ItfARF10b were expressed in both root types with moderate to high expression for each. All ten of these ARF genes and their prominent expression signify their importance within the development of each respective root type. This study provides comprehensive information regarding the ARF family in sweet potatoes, which will be useful for future research to discover further functional verification of these ItfARF genes. MDPI 2021-08-30 /pmc/articles/PMC8431151/ /pubmed/34502298 http://dx.doi.org/10.3390/ijms22179391 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Pratt, Isaac Seth Zhang, Baohong Genome-Wide Identification of ARF Transcription Factor Gene Family and Their Expression Analysis in Sweet Potato |
title | Genome-Wide Identification of ARF Transcription Factor Gene Family and Their Expression Analysis in Sweet Potato |
title_full | Genome-Wide Identification of ARF Transcription Factor Gene Family and Their Expression Analysis in Sweet Potato |
title_fullStr | Genome-Wide Identification of ARF Transcription Factor Gene Family and Their Expression Analysis in Sweet Potato |
title_full_unstemmed | Genome-Wide Identification of ARF Transcription Factor Gene Family and Their Expression Analysis in Sweet Potato |
title_short | Genome-Wide Identification of ARF Transcription Factor Gene Family and Their Expression Analysis in Sweet Potato |
title_sort | genome-wide identification of arf transcription factor gene family and their expression analysis in sweet potato |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8431151/ https://www.ncbi.nlm.nih.gov/pubmed/34502298 http://dx.doi.org/10.3390/ijms22179391 |
work_keys_str_mv | AT prattisaacseth genomewideidentificationofarftranscriptionfactorgenefamilyandtheirexpressionanalysisinsweetpotato AT zhangbaohong genomewideidentificationofarftranscriptionfactorgenefamilyandtheirexpressionanalysisinsweetpotato |