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Influence of Vitamin D on the Vasoactive Effect of Estradiol in a Rat Model of Polycystic Ovary Syndrome
We examined the vasoactive effect of estradiol in a rat model of early PCOS and the influence of vitamin D deficiency (VDD). We created a model of chronic hyperandrogenism and VDD in adolescent female Wistar rats (N = 46) with four experimental groups: vitamin D supplemented (T-D+), VDD (T-D-), hype...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8431242/ https://www.ncbi.nlm.nih.gov/pubmed/34502321 http://dx.doi.org/10.3390/ijms22179404 |
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author | Tarszabó, Róbert Bányai, Bálint Ruisanchez, Éva Péterffy, Borbála Korsós-Novák, Ágnes Lajtai, Krisztina Sziva, Réka Eszter Gerszi, Dóra Hosszú, Ádám Benkő, Rita Benyó, Zoltán Horváth, Eszter Mária Masszi, Gabriella Várbíró, Szabolcs |
author_facet | Tarszabó, Róbert Bányai, Bálint Ruisanchez, Éva Péterffy, Borbála Korsós-Novák, Ágnes Lajtai, Krisztina Sziva, Réka Eszter Gerszi, Dóra Hosszú, Ádám Benkő, Rita Benyó, Zoltán Horváth, Eszter Mária Masszi, Gabriella Várbíró, Szabolcs |
author_sort | Tarszabó, Róbert |
collection | PubMed |
description | We examined the vasoactive effect of estradiol in a rat model of early PCOS and the influence of vitamin D deficiency (VDD). We created a model of chronic hyperandrogenism and VDD in adolescent female Wistar rats (N = 46) with four experimental groups: vitamin D supplemented (T-D+), VDD (T-D-), hyperandrogenic and vitamin D supplemented (T+D+), and hyperandrogenic and VDD (T+D-). T+ groups received an 8-week-long transdermal Androgel treatment, D-animals were on vitamin D-reduced diet and D+ rats were supplemented orally with vitamin D3. Estrogen-induced vasorelaxation of thoracic aorta segments were measured with a wire myograph system with or without the inhibition of endothelial nitric oxide synthase (eNOS) or cyclooxygenase-2 (COX-2). The distribution of estrogen receptor (ER), eNOS and COX-2 in the aortic wall was assessed by immunohistochemistry. VDD aortas showed significantly lower estradiol-induced relaxation independently of androgenic status that was further decreased by COX-2 inhibition. COX-2 inhibition failed to alter vessel function in D+ rats. Inhibition of eNOS abolished the estradiol-induced relaxation in all groups. Changes in vascular function in VDD were accompanied by significantly decreased ER and eNOS staining. Short-term chronic hyperandrogenism failed to, but VDD induced vascular dysfunction, compromised estrogen-dependent vasodilatation and changes in ER and eNOS immunostaining. |
format | Online Article Text |
id | pubmed-8431242 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-84312422021-09-11 Influence of Vitamin D on the Vasoactive Effect of Estradiol in a Rat Model of Polycystic Ovary Syndrome Tarszabó, Róbert Bányai, Bálint Ruisanchez, Éva Péterffy, Borbála Korsós-Novák, Ágnes Lajtai, Krisztina Sziva, Réka Eszter Gerszi, Dóra Hosszú, Ádám Benkő, Rita Benyó, Zoltán Horváth, Eszter Mária Masszi, Gabriella Várbíró, Szabolcs Int J Mol Sci Article We examined the vasoactive effect of estradiol in a rat model of early PCOS and the influence of vitamin D deficiency (VDD). We created a model of chronic hyperandrogenism and VDD in adolescent female Wistar rats (N = 46) with four experimental groups: vitamin D supplemented (T-D+), VDD (T-D-), hyperandrogenic and vitamin D supplemented (T+D+), and hyperandrogenic and VDD (T+D-). T+ groups received an 8-week-long transdermal Androgel treatment, D-animals were on vitamin D-reduced diet and D+ rats were supplemented orally with vitamin D3. Estrogen-induced vasorelaxation of thoracic aorta segments were measured with a wire myograph system with or without the inhibition of endothelial nitric oxide synthase (eNOS) or cyclooxygenase-2 (COX-2). The distribution of estrogen receptor (ER), eNOS and COX-2 in the aortic wall was assessed by immunohistochemistry. VDD aortas showed significantly lower estradiol-induced relaxation independently of androgenic status that was further decreased by COX-2 inhibition. COX-2 inhibition failed to alter vessel function in D+ rats. Inhibition of eNOS abolished the estradiol-induced relaxation in all groups. Changes in vascular function in VDD were accompanied by significantly decreased ER and eNOS staining. Short-term chronic hyperandrogenism failed to, but VDD induced vascular dysfunction, compromised estrogen-dependent vasodilatation and changes in ER and eNOS immunostaining. MDPI 2021-08-30 /pmc/articles/PMC8431242/ /pubmed/34502321 http://dx.doi.org/10.3390/ijms22179404 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Tarszabó, Róbert Bányai, Bálint Ruisanchez, Éva Péterffy, Borbála Korsós-Novák, Ágnes Lajtai, Krisztina Sziva, Réka Eszter Gerszi, Dóra Hosszú, Ádám Benkő, Rita Benyó, Zoltán Horváth, Eszter Mária Masszi, Gabriella Várbíró, Szabolcs Influence of Vitamin D on the Vasoactive Effect of Estradiol in a Rat Model of Polycystic Ovary Syndrome |
title | Influence of Vitamin D on the Vasoactive Effect of Estradiol in a Rat Model of Polycystic Ovary Syndrome |
title_full | Influence of Vitamin D on the Vasoactive Effect of Estradiol in a Rat Model of Polycystic Ovary Syndrome |
title_fullStr | Influence of Vitamin D on the Vasoactive Effect of Estradiol in a Rat Model of Polycystic Ovary Syndrome |
title_full_unstemmed | Influence of Vitamin D on the Vasoactive Effect of Estradiol in a Rat Model of Polycystic Ovary Syndrome |
title_short | Influence of Vitamin D on the Vasoactive Effect of Estradiol in a Rat Model of Polycystic Ovary Syndrome |
title_sort | influence of vitamin d on the vasoactive effect of estradiol in a rat model of polycystic ovary syndrome |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8431242/ https://www.ncbi.nlm.nih.gov/pubmed/34502321 http://dx.doi.org/10.3390/ijms22179404 |
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