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αV Integrin Expression and Localization in Male Germ Cells
Integrins are transmembrane receptors that facilitate cell adhesion and cell–extracellular matrix communication. They are involved in the sperm maturation including capacitation and gamete interaction, resulting in successful fertilization. αV integrin belongs to the integrin glycoprotein superfamil...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8431249/ https://www.ncbi.nlm.nih.gov/pubmed/34502434 http://dx.doi.org/10.3390/ijms22179525 |
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author | Palenikova, Veronika Frolikova, Michaela Valaskova, Eliska Postlerova, Pavla Komrskova, Katerina |
author_facet | Palenikova, Veronika Frolikova, Michaela Valaskova, Eliska Postlerova, Pavla Komrskova, Katerina |
author_sort | Palenikova, Veronika |
collection | PubMed |
description | Integrins are transmembrane receptors that facilitate cell adhesion and cell–extracellular matrix communication. They are involved in the sperm maturation including capacitation and gamete interaction, resulting in successful fertilization. αV integrin belongs to the integrin glycoprotein superfamily, and it is indispensable for physiological spermiogenesis and testosterone production. We targeted the gene and protein expression of the αV integrin subunit and described its membrane localization in sperm. Firstly, in mouse, we traced αV integrin gene expression during spermatogenesis in testicular fraction separated by elutriation, and we detected gene activity in spermatogonia, spermatocytes, and round spermatids. Secondly, we specified αV integrin membrane localization in acrosome-intact and acrosome-reacted sperm and compared its pattern between mouse, pig, and human. Using immunodetection and structured illumination microscopy (SIM), the αV integrin localization was confined to the plasma membrane covering the acrosomal cap area and also to the inner acrosomal membrane of acrosome-intact sperm of all selected species. During the acrosome reaction, which was induced on capacitated sperm, the αV integrin relocated and was detected over the whole sperm head. Knowledge of the integrin pattern in mature sperm prepares the ground for further investigation into the pathologies and related fertility issues in human medicine and veterinary science. |
format | Online Article Text |
id | pubmed-8431249 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-84312492021-09-11 αV Integrin Expression and Localization in Male Germ Cells Palenikova, Veronika Frolikova, Michaela Valaskova, Eliska Postlerova, Pavla Komrskova, Katerina Int J Mol Sci Article Integrins are transmembrane receptors that facilitate cell adhesion and cell–extracellular matrix communication. They are involved in the sperm maturation including capacitation and gamete interaction, resulting in successful fertilization. αV integrin belongs to the integrin glycoprotein superfamily, and it is indispensable for physiological spermiogenesis and testosterone production. We targeted the gene and protein expression of the αV integrin subunit and described its membrane localization in sperm. Firstly, in mouse, we traced αV integrin gene expression during spermatogenesis in testicular fraction separated by elutriation, and we detected gene activity in spermatogonia, spermatocytes, and round spermatids. Secondly, we specified αV integrin membrane localization in acrosome-intact and acrosome-reacted sperm and compared its pattern between mouse, pig, and human. Using immunodetection and structured illumination microscopy (SIM), the αV integrin localization was confined to the plasma membrane covering the acrosomal cap area and also to the inner acrosomal membrane of acrosome-intact sperm of all selected species. During the acrosome reaction, which was induced on capacitated sperm, the αV integrin relocated and was detected over the whole sperm head. Knowledge of the integrin pattern in mature sperm prepares the ground for further investigation into the pathologies and related fertility issues in human medicine and veterinary science. MDPI 2021-09-02 /pmc/articles/PMC8431249/ /pubmed/34502434 http://dx.doi.org/10.3390/ijms22179525 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Palenikova, Veronika Frolikova, Michaela Valaskova, Eliska Postlerova, Pavla Komrskova, Katerina αV Integrin Expression and Localization in Male Germ Cells |
title | αV Integrin Expression and Localization in Male Germ Cells |
title_full | αV Integrin Expression and Localization in Male Germ Cells |
title_fullStr | αV Integrin Expression and Localization in Male Germ Cells |
title_full_unstemmed | αV Integrin Expression and Localization in Male Germ Cells |
title_short | αV Integrin Expression and Localization in Male Germ Cells |
title_sort | αv integrin expression and localization in male germ cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8431249/ https://www.ncbi.nlm.nih.gov/pubmed/34502434 http://dx.doi.org/10.3390/ijms22179525 |
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