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Cereulide Exposure Caused Cytopathogenic Damages of Liver and Kidney in Mice

Cereulide is one of the main food-borne toxins for vomiting synthesized by Bacillus cereus, and it widely contaminates meat, eggs, milk, and starchy foods. However, the toxicological effects and mechanisms of the long-time exposure of cereulide in vivo remain unknown. In this study, oral administrat...

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Autores principales: Li, Danyang, Lin, Ruqin, Xu, Yangyang, Chen, Qingmei, Deng, Fengru, Deng, Yiqun, Wen, Jikai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8431326/
https://www.ncbi.nlm.nih.gov/pubmed/34502057
http://dx.doi.org/10.3390/ijms22179148
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author Li, Danyang
Lin, Ruqin
Xu, Yangyang
Chen, Qingmei
Deng, Fengru
Deng, Yiqun
Wen, Jikai
author_facet Li, Danyang
Lin, Ruqin
Xu, Yangyang
Chen, Qingmei
Deng, Fengru
Deng, Yiqun
Wen, Jikai
author_sort Li, Danyang
collection PubMed
description Cereulide is one of the main food-borne toxins for vomiting synthesized by Bacillus cereus, and it widely contaminates meat, eggs, milk, and starchy foods. However, the toxicological effects and mechanisms of the long-time exposure of cereulide in vivo remain unknown. In this study, oral administration of 50 and 200 μg/kg body weight cereulide in the mice for 28 days caused oxidative stress in liver and kidney tissues and induce abnormal expression of inflammatory factors. In pathogenesis, cereulide exposure activated endoplasmic reticulum stress (ER stress) via the pathways of inositol-requiring enzyme 1α (IRE1α)/Xbox binding protein (XBP1) and PRKR-like ER kinase (PERK)/eukaryotic translation initiation factor 2α (eIF2α), and consequently led to the apoptosis and tissue damages in mouse liver and kidney. In vitro, we confirmed that the accumulation of reactive oxygen species (ROS) caused by cereulide is the main factor leading to ER stress in HepaRG and HEK293T cells. Supplementation of sodium butyrate (NaB) inhibited the activations of IRE1α/XBP1 and PERK/eIF2α pathways caused by cereulide exposure in mice, and reduced the cell apoptosis in liver and kidney. In conclusion, this study provides a new insight in understanding the toxicological mechanism and prevention of cereulide exposure.
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spelling pubmed-84313262021-09-11 Cereulide Exposure Caused Cytopathogenic Damages of Liver and Kidney in Mice Li, Danyang Lin, Ruqin Xu, Yangyang Chen, Qingmei Deng, Fengru Deng, Yiqun Wen, Jikai Int J Mol Sci Article Cereulide is one of the main food-borne toxins for vomiting synthesized by Bacillus cereus, and it widely contaminates meat, eggs, milk, and starchy foods. However, the toxicological effects and mechanisms of the long-time exposure of cereulide in vivo remain unknown. In this study, oral administration of 50 and 200 μg/kg body weight cereulide in the mice for 28 days caused oxidative stress in liver and kidney tissues and induce abnormal expression of inflammatory factors. In pathogenesis, cereulide exposure activated endoplasmic reticulum stress (ER stress) via the pathways of inositol-requiring enzyme 1α (IRE1α)/Xbox binding protein (XBP1) and PRKR-like ER kinase (PERK)/eukaryotic translation initiation factor 2α (eIF2α), and consequently led to the apoptosis and tissue damages in mouse liver and kidney. In vitro, we confirmed that the accumulation of reactive oxygen species (ROS) caused by cereulide is the main factor leading to ER stress in HepaRG and HEK293T cells. Supplementation of sodium butyrate (NaB) inhibited the activations of IRE1α/XBP1 and PERK/eIF2α pathways caused by cereulide exposure in mice, and reduced the cell apoptosis in liver and kidney. In conclusion, this study provides a new insight in understanding the toxicological mechanism and prevention of cereulide exposure. MDPI 2021-08-24 /pmc/articles/PMC8431326/ /pubmed/34502057 http://dx.doi.org/10.3390/ijms22179148 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Li, Danyang
Lin, Ruqin
Xu, Yangyang
Chen, Qingmei
Deng, Fengru
Deng, Yiqun
Wen, Jikai
Cereulide Exposure Caused Cytopathogenic Damages of Liver and Kidney in Mice
title Cereulide Exposure Caused Cytopathogenic Damages of Liver and Kidney in Mice
title_full Cereulide Exposure Caused Cytopathogenic Damages of Liver and Kidney in Mice
title_fullStr Cereulide Exposure Caused Cytopathogenic Damages of Liver and Kidney in Mice
title_full_unstemmed Cereulide Exposure Caused Cytopathogenic Damages of Liver and Kidney in Mice
title_short Cereulide Exposure Caused Cytopathogenic Damages of Liver and Kidney in Mice
title_sort cereulide exposure caused cytopathogenic damages of liver and kidney in mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8431326/
https://www.ncbi.nlm.nih.gov/pubmed/34502057
http://dx.doi.org/10.3390/ijms22179148
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