Cargando…

Ultra-Rapid Drug Susceptibility Testing for Klebsiella pneumoniae Clinical Isolates in 60 Min by SYBR Green I/Propidium Iodide Viability Assay

BACKGROUND: We aimed to optimize and validate the drug susceptibility test (DST) assay by SYBR Green I/PI (SG-PI) method using a panel of 89 Klebsiella pneumoniae clinical isolates in comparison with the conventional DST method to three most important antibiotics used for treatment of this bacterial...

Descripción completa

Detalles Bibliográficos
Autores principales:	Chen, Jiazhen, Wang, Xuyang, Wang, Shiyong, Chen, Chen, Zhang, Wenhong, Zhang, Ying
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Frontiers Media S.A. 2021
Materias:	Microbiology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8431398/ https://www.ncbi.nlm.nih.gov/pubmed/34512572 http://dx.doi.org/10.3389/fmicb.2021.694522

Ejemplares similares

A Rapid Growth-Independent Antibiotic Resistance Detection Test by SYBR Green/Propidium Iodide Viability Assay
por: Feng, Jie, et al.
Publicado: (2018)

Rapid Determination of Antibiotic Resistance in Klebsiella pneumoniae by a Novel Antibiotic Susceptibility Testing Method Using SYBR Green I and Propidium Iodide Double Staining
por: Zhang, Yabin, et al.
Publicado: (2021)

Propidium iodide staining underestimates viability of adherent bacterial cells
por: Rosenberg, Merilin, et al.
Publicado: (2019)

Critical aspects of using bacterial cell viability assays with the fluorophores SYTO9 and propidium iodide
por: Stiefel, Philipp, et al.
Publicado: (2015)

From red to green: the propidium iodide-permeable membrane of Shewanella decolorationis S12 is repairable
por: Yang, Yonggang, et al.
Publicado: (2015)

Rapid flow cytofluorometric analysis of mammalian cell cycle by propidium iodide staining
Publicado: (1975)

The DNA intercalators ethidium bromide and propidium iodide also bind to core histones
por: Banerjee, Amrita, et al.
Publicado: (2014)

Modified Pseudo-Schiff Propidium Iodide for Staining the Shoot Apical Meristem in Arabidopsis
por: Li, Ruiqi, et al.
Publicado: (2023)

An Optimized SYBR Green I/PI Assay for Rapid Viability Assessment and Antibiotic Susceptibility Testing for Borrelia burgdorferi
por: Feng, Jie, et al.
Publicado: (2014)

Modified Annexin V/Propidium Iodide Apoptosis Assay For Accurate Assessment of Cell Death
por: Rieger, Aja M., et al.
Publicado: (2011)

Corrigendum to: The DNA intercalators ethidium bromide and propidium iodide also bind to core histones
Publicado: (2022)

Whole microbial community viability is not quantitatively reflected by propidium monoazide sequencing approach
por: Wang, Ya, et al.
Publicado: (2021)

Involvement of Small Colony Variant-Related Heme Biosynthesis Genes in Staphylococcus aureus Persister Formation in vitro
por: Wang, Xuyang, et al.
Publicado: (2021)

Flow cytofluorometric analysis of cell cycle distributions using propidium iodide. Properties of the method and mathematical analysis of the data
Publicado: (1976)

The antibacterial efficacy of silver diamine fluoride (SDF) is not modulated by potassium iodide (KI) supplements: A study on in-situ plaque biofilms using viability real-time PCR with propidium monoazide
por: Abdullah, Nizam, et al.
Publicado: (2020)

Rapid molecular phenotypic antimicrobial susceptibility test for Neisseria gonorrhoeae based on propidium monoazide viability PCR
por: Tjandra, Kristel C., et al.
Publicado: (2023)

Fluorescence detection of malachite green in fish based on aptamer and SYBR Green I
por: Wang, Jia, et al.
Publicado: (2022)

Determination of the viability of Toxoplasma gondii oocysts by PCR real-time after treatment with propidium monoazide
por: Marciano, Maria Aparecida Moraes, et al.
Publicado: (2020)

Rapid visual detection of anisakid nematodes using recombinase polymerase amplification and SYBR Green I
por: Chen, Xiuqin, et al.
Publicado: (2022)

Energy Transfer between Fluorescein Isothiocyanate and Propidium Iodide – A Problem in the Estimation of T(pot) with the Bromodeoxyuridine–DNA Flow Cytometry Technique?
por: Johansson, Maria C., et al.
Publicado: (1999)

Time-resolved, single-cell analysis of induced and programmed cell death via non-invasive propidium iodide and counterstain perfusion
por: Krämer, Christina E. M., et al.
Publicado: (2016)

Assessment of Probiotic Viability during Cheddar Cheese Manufacture and Ripening Using Propidium Monoazide-PCR Quantification
por: Desfossés-Foucault, Émilie, et al.
Publicado: (2012)

SYTO dyes and EvaGreen outperform SYBR Green in real-time PCR
por: Eischeid, Anne C
Publicado: (2011)

Synergistic Effect of Propidium Iodide and Small Molecule Antibiotics with the Antimicrobial Peptide Dendrimer G3KL against Gram-Negative Bacteria
por: Gan, Bee-Ha, et al.
Publicado: (2020)

Evaluation of Whole-Genome Sequence Method to Diagnose Resistance of 13 Anti-tuberculosis Drugs and Characterize Resistance Genes in Clinical Multi-Drug Resistance Mycobacterium tuberculosis Isolates From China
por: Chen, Xinchang, et al.
Publicado: (2019)

Corrigendum: Evaluation of Whole-Genome Sequence Method to Diagnose Resistance of 13 Anti-tuberculosis Drugs and Characterize Resistance Genes in Clinical Multi-Drug Resistance Mycobacterium tuberculosis Isolates From China
por: Chen, Xinchang, et al.
Publicado: (2019)

Viability of Legionella pneumophila in Water Samples: A Comparison of Propidium Monoazide (PMA) Treatment on Membrane Filters and in Liquid
por: Bonetta, Sara, et al.
Publicado: (2017)

Development of a Propidium Monoazide-Based Viability Quantitative PCR Assay for Red Sea Bream Iridovirus Detection
por: Kim, Kyung-Ho, et al.
Publicado: (2023)

Development of a SYBR green real-time PCR method for rapid detection of sheep pox virus
por: Tian, Hong, et al.
Publicado: (2012)

Analysis of Serial Multidrug-Resistant Tuberculosis Strains Causing Treatment Failure and Within-Host Evolution by Whole-Genome Sequencing
por: Chen, Xinchang, et al.
Publicado: (2020)

Development of a SYBR Green I real-time PCR for the detection of the orf virus
por: Wang, Yong, et al.
Publicado: (2017)

Development of SYBR Green I-based polymerase chain reaction for feline bocavirus 1 detection
por: Wang, Yong, et al.
Publicado: (2021)

The effect of injection speed and serial injection on propidium iodide entry into cultured HeLa and primary neonatal fibroblast cells using lance array nanoinjection
por: Sessions, John W., et al.
Publicado: (2016)

Development and application of a SYBR green real-time PCR for detection of the emerging avian leukosis virus subgroup K
por: Chen, Jian, et al.
Publicado: (2018)

The development of a rapid SYBR Green I-based quantitative PCR for detection of Duck circovirus
por: Wan, Chunhe, et al.
Publicado: (2011)

In vitro Susceptibility of Nontuberculous Mycobacteria to Tedizolid
por: Zhang, Huiyun, et al.
Publicado: (2022)

Development and evaluation of a SYBR Green real-time RT-PCR assay for detection of avian hepatitis E virus
por: Zhao, Qin, et al.
Publicado: (2015)

Drying anti-malarial drugs in vitro tests to outsource SYBR green assays
por: Traore, Karim, et al.
Publicado: (2015)

Multiplex SYBR Green Real-Time PCR Assay for Detection of Respiratory Viruses
por: Sultani, Mozhdeh, et al.
Publicado: (2015)

Bovine ringworm - Detection of Trichophyton verrucosum by SYBR-Green real-time PCR
por: Spanamberg, Andréia, et al.
Publicado: (2023)

Cannot write session to /tmp/vufind_sessions/sess_akhc74mjghshfdmis3ev2mq3he