Involvement of Reactive Oxygen Species in ABA-Induced Increase in Hydraulic Conductivity and Aquaporin Abundance

The role of reactive oxygen species (ROS) in ABA-induced increase in hydraulic conductivity was hypothesized to be dependent on an increase in aquaporin water channel (AQP) abundance. Single ABA application or its combination with ROS manipulators (ROS scavenger ascorbic acid and NADPH oxidase inhib...

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Autores principales: Sharipova, Guzel, Ivanov, Ruslan, Veselov, Dmitriy, Akhiyarova, Guzel, Shishova, Maria, Nuzhnaya, Tatyana, Kudoyarova, Guzel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8431682/
https://www.ncbi.nlm.nih.gov/pubmed/34502052
http://dx.doi.org/10.3390/ijms22179144
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author Sharipova, Guzel
Ivanov, Ruslan
Veselov, Dmitriy
Akhiyarova, Guzel
Shishova, Maria
Nuzhnaya, Tatyana
Kudoyarova, Guzel
author_facet Sharipova, Guzel
Ivanov, Ruslan
Veselov, Dmitriy
Akhiyarova, Guzel
Shishova, Maria
Nuzhnaya, Tatyana
Kudoyarova, Guzel
author_sort Sharipova, Guzel
collection PubMed
description The role of reactive oxygen species (ROS) in ABA-induced increase in hydraulic conductivity was hypothesized to be dependent on an increase in aquaporin water channel (AQP) abundance. Single ABA application or its combination with ROS manipulators (ROS scavenger ascorbic acid and NADPH oxidase inhibitor diphenyleneiodonium chloride (DPI)) were studied on detached roots of barley plants. We measured the osmotically driven flow rate of xylem sap and calculated root hydraulic conductivity. In parallel, immunolocalization of ABA and HvPIP2;2 AQPs was performed with corresponding specific antibodies. ABA treatment increased the flow rate of xylem, root hydraulic conductivity and immunostaining for ABA and HvPIP2;2, while the addition of antioxidants prevented the effects of this hormone. The obtained results confirmed the involvement of ROS in ABA effect on hydraulic conductivity, in particular, the importance of H(2)O(2) production by ABA-treated plants for the effect of this hormone on AQP abundance.
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spelling pubmed-84316822021-09-11 Involvement of Reactive Oxygen Species in ABA-Induced Increase in Hydraulic Conductivity and Aquaporin Abundance Sharipova, Guzel Ivanov, Ruslan Veselov, Dmitriy Akhiyarova, Guzel Shishova, Maria Nuzhnaya, Tatyana Kudoyarova, Guzel Int J Mol Sci Communication The role of reactive oxygen species (ROS) in ABA-induced increase in hydraulic conductivity was hypothesized to be dependent on an increase in aquaporin water channel (AQP) abundance. Single ABA application or its combination with ROS manipulators (ROS scavenger ascorbic acid and NADPH oxidase inhibitor diphenyleneiodonium chloride (DPI)) were studied on detached roots of barley plants. We measured the osmotically driven flow rate of xylem sap and calculated root hydraulic conductivity. In parallel, immunolocalization of ABA and HvPIP2;2 AQPs was performed with corresponding specific antibodies. ABA treatment increased the flow rate of xylem, root hydraulic conductivity and immunostaining for ABA and HvPIP2;2, while the addition of antioxidants prevented the effects of this hormone. The obtained results confirmed the involvement of ROS in ABA effect on hydraulic conductivity, in particular, the importance of H(2)O(2) production by ABA-treated plants for the effect of this hormone on AQP abundance. MDPI 2021-08-24 /pmc/articles/PMC8431682/ /pubmed/34502052 http://dx.doi.org/10.3390/ijms22179144 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Sharipova, Guzel
Ivanov, Ruslan
Veselov, Dmitriy
Akhiyarova, Guzel
Shishova, Maria
Nuzhnaya, Tatyana
Kudoyarova, Guzel
Involvement of Reactive Oxygen Species in ABA-Induced Increase in Hydraulic Conductivity and Aquaporin Abundance
title Involvement of Reactive Oxygen Species in ABA-Induced Increase in Hydraulic Conductivity and Aquaporin Abundance
title_full Involvement of Reactive Oxygen Species in ABA-Induced Increase in Hydraulic Conductivity and Aquaporin Abundance
title_fullStr Involvement of Reactive Oxygen Species in ABA-Induced Increase in Hydraulic Conductivity and Aquaporin Abundance
title_full_unstemmed Involvement of Reactive Oxygen Species in ABA-Induced Increase in Hydraulic Conductivity and Aquaporin Abundance
title_short Involvement of Reactive Oxygen Species in ABA-Induced Increase in Hydraulic Conductivity and Aquaporin Abundance
title_sort involvement of reactive oxygen species in aba-induced increase in hydraulic conductivity and aquaporin abundance
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8431682/
https://www.ncbi.nlm.nih.gov/pubmed/34502052
http://dx.doi.org/10.3390/ijms22179144
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