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GhTBL34 Is Associated with Verticillium Wilt Resistance in Cotton

Verticillium wilt (VW) is a typical fungal disease affecting the yield and quality of cotton. The Trichome Birefringence-Like protein (TBL) is an acetyltransferase involved in the acetylation process of cell wall polysaccharides. Up to now, there are no reports on whether the TBL gene is related to...

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Autores principales: Zhao, Yunlei, Jing, Huijuan, Zhao, Pei, Chen, Wei, Li, Xuelin, Sang, Xiaohui, Lu, Jianhua, Wang, Hongmei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8431740/
https://www.ncbi.nlm.nih.gov/pubmed/34502024
http://dx.doi.org/10.3390/ijms22179115
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author Zhao, Yunlei
Jing, Huijuan
Zhao, Pei
Chen, Wei
Li, Xuelin
Sang, Xiaohui
Lu, Jianhua
Wang, Hongmei
author_facet Zhao, Yunlei
Jing, Huijuan
Zhao, Pei
Chen, Wei
Li, Xuelin
Sang, Xiaohui
Lu, Jianhua
Wang, Hongmei
author_sort Zhao, Yunlei
collection PubMed
description Verticillium wilt (VW) is a typical fungal disease affecting the yield and quality of cotton. The Trichome Birefringence-Like protein (TBL) is an acetyltransferase involved in the acetylation process of cell wall polysaccharides. Up to now, there are no reports on whether the TBL gene is related to disease resistance in cotton. In this study, we cloned a cotton TBL34 gene located in the confidence interval of a major VW resistance quantitative trait loci and demonstrated its relationship with VW resistance in cotton. Analyzing the sequence variations in resistant and susceptible accessions detected two elite alleles GhTBL34-2 and GhTBL34-3, mainly presented in resistant cotton lines whose disease index was significantly lower than that of susceptible lines carrying the allele GhTBL34-1. Comparing the TBL34 protein sequences showed that two amino acid differences in the TBL (PMR5N) domain changed the susceptible allele GhTBL34-1 into the resistant allele GhTBL34-2 (GhTBL34-3). Expression analysis showed that the TBL34 was obviously up-regulated by infection of Verticillium dahliae and exogenous treatment of ethylene (ET), and salicylic acid (SA) and jasmonate (JA) in cotton. VIGS experiments demonstrated that silencing of TBL34 reduced VW resistance in cotton. We deduced that the TBL34 gene mediating acetylation of cell wall polysaccharides might be involved in the regulation of resistance to VW in cotton.
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spelling pubmed-84317402021-09-11 GhTBL34 Is Associated with Verticillium Wilt Resistance in Cotton Zhao, Yunlei Jing, Huijuan Zhao, Pei Chen, Wei Li, Xuelin Sang, Xiaohui Lu, Jianhua Wang, Hongmei Int J Mol Sci Article Verticillium wilt (VW) is a typical fungal disease affecting the yield and quality of cotton. The Trichome Birefringence-Like protein (TBL) is an acetyltransferase involved in the acetylation process of cell wall polysaccharides. Up to now, there are no reports on whether the TBL gene is related to disease resistance in cotton. In this study, we cloned a cotton TBL34 gene located in the confidence interval of a major VW resistance quantitative trait loci and demonstrated its relationship with VW resistance in cotton. Analyzing the sequence variations in resistant and susceptible accessions detected two elite alleles GhTBL34-2 and GhTBL34-3, mainly presented in resistant cotton lines whose disease index was significantly lower than that of susceptible lines carrying the allele GhTBL34-1. Comparing the TBL34 protein sequences showed that two amino acid differences in the TBL (PMR5N) domain changed the susceptible allele GhTBL34-1 into the resistant allele GhTBL34-2 (GhTBL34-3). Expression analysis showed that the TBL34 was obviously up-regulated by infection of Verticillium dahliae and exogenous treatment of ethylene (ET), and salicylic acid (SA) and jasmonate (JA) in cotton. VIGS experiments demonstrated that silencing of TBL34 reduced VW resistance in cotton. We deduced that the TBL34 gene mediating acetylation of cell wall polysaccharides might be involved in the regulation of resistance to VW in cotton. MDPI 2021-08-24 /pmc/articles/PMC8431740/ /pubmed/34502024 http://dx.doi.org/10.3390/ijms22179115 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhao, Yunlei
Jing, Huijuan
Zhao, Pei
Chen, Wei
Li, Xuelin
Sang, Xiaohui
Lu, Jianhua
Wang, Hongmei
GhTBL34 Is Associated with Verticillium Wilt Resistance in Cotton
title GhTBL34 Is Associated with Verticillium Wilt Resistance in Cotton
title_full GhTBL34 Is Associated with Verticillium Wilt Resistance in Cotton
title_fullStr GhTBL34 Is Associated with Verticillium Wilt Resistance in Cotton
title_full_unstemmed GhTBL34 Is Associated with Verticillium Wilt Resistance in Cotton
title_short GhTBL34 Is Associated with Verticillium Wilt Resistance in Cotton
title_sort ghtbl34 is associated with verticillium wilt resistance in cotton
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8431740/
https://www.ncbi.nlm.nih.gov/pubmed/34502024
http://dx.doi.org/10.3390/ijms22179115
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