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Utility of Platelet Endothelial Cell Adhesion Molecule 1 in the Platelet Activity Assessment in Mouse and Human Blood

In our previous study, we introduced the platelet endothelial cell adhesion molecule 1 (PECAM-1)/thrombus ratio, which is a parameter indicating the proportion of PECAM-1 in laser-induced thrombi in mice. Because PECAM-1 is an antithrombotic molecule, the higher the PECAM-1/thrombus ratio, the less...

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Autores principales: Marcinczyk, Natalia, Misztal, Tomasz, Gromotowicz-Poplawska, Anna, Zebrowska, Agnieszka, Rusak, Tomasz, Radziwon, Piotr, Chabielska, Ewa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8431756/
https://www.ncbi.nlm.nih.gov/pubmed/34502520
http://dx.doi.org/10.3390/ijms22179611
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author Marcinczyk, Natalia
Misztal, Tomasz
Gromotowicz-Poplawska, Anna
Zebrowska, Agnieszka
Rusak, Tomasz
Radziwon, Piotr
Chabielska, Ewa
author_facet Marcinczyk, Natalia
Misztal, Tomasz
Gromotowicz-Poplawska, Anna
Zebrowska, Agnieszka
Rusak, Tomasz
Radziwon, Piotr
Chabielska, Ewa
author_sort Marcinczyk, Natalia
collection PubMed
description In our previous study, we introduced the platelet endothelial cell adhesion molecule 1 (PECAM-1)/thrombus ratio, which is a parameter indicating the proportion of PECAM-1 in laser-induced thrombi in mice. Because PECAM-1 is an antithrombotic molecule, the higher the PECAM-1/thrombus ratio, the less activated the platelets. In this study, we used an extracorporeal model of thrombosis (flow chamber model) to verify its usefulness in the assessment of the PECAM-1/thrombus ratio in animal and human studies. Using the lipopolysaccharide (LPS)-induced inflammation model, we also evaluated whether the PECAM-1/thrombus ratio determined in the flow chamber (without endothelium) differed from that calculated in laser-induced thrombosis (with endothelium). We observed that acetylsalicylic acid (ASA) decreased the area of the thrombus while increasing the PECAM-1/thrombus ratio in healthy mice and humans in a dose-dependent manner. In LPS-treated mice, the PECAM-1/thrombus ratio decreased as the dose of ASA increased in both thrombosis models, but the direction of change in the thrombus area was inconsistent. Our study demonstrates that the PECAM-1/thrombus ratio can more accurately describe the platelet activation status than commonly used parameters such as the thrombus area, and, hence, it can be used in both human and animal studies.
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spelling pubmed-84317562021-09-11 Utility of Platelet Endothelial Cell Adhesion Molecule 1 in the Platelet Activity Assessment in Mouse and Human Blood Marcinczyk, Natalia Misztal, Tomasz Gromotowicz-Poplawska, Anna Zebrowska, Agnieszka Rusak, Tomasz Radziwon, Piotr Chabielska, Ewa Int J Mol Sci Communication In our previous study, we introduced the platelet endothelial cell adhesion molecule 1 (PECAM-1)/thrombus ratio, which is a parameter indicating the proportion of PECAM-1 in laser-induced thrombi in mice. Because PECAM-1 is an antithrombotic molecule, the higher the PECAM-1/thrombus ratio, the less activated the platelets. In this study, we used an extracorporeal model of thrombosis (flow chamber model) to verify its usefulness in the assessment of the PECAM-1/thrombus ratio in animal and human studies. Using the lipopolysaccharide (LPS)-induced inflammation model, we also evaluated whether the PECAM-1/thrombus ratio determined in the flow chamber (without endothelium) differed from that calculated in laser-induced thrombosis (with endothelium). We observed that acetylsalicylic acid (ASA) decreased the area of the thrombus while increasing the PECAM-1/thrombus ratio in healthy mice and humans in a dose-dependent manner. In LPS-treated mice, the PECAM-1/thrombus ratio decreased as the dose of ASA increased in both thrombosis models, but the direction of change in the thrombus area was inconsistent. Our study demonstrates that the PECAM-1/thrombus ratio can more accurately describe the platelet activation status than commonly used parameters such as the thrombus area, and, hence, it can be used in both human and animal studies. MDPI 2021-09-04 /pmc/articles/PMC8431756/ /pubmed/34502520 http://dx.doi.org/10.3390/ijms22179611 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Marcinczyk, Natalia
Misztal, Tomasz
Gromotowicz-Poplawska, Anna
Zebrowska, Agnieszka
Rusak, Tomasz
Radziwon, Piotr
Chabielska, Ewa
Utility of Platelet Endothelial Cell Adhesion Molecule 1 in the Platelet Activity Assessment in Mouse and Human Blood
title Utility of Platelet Endothelial Cell Adhesion Molecule 1 in the Platelet Activity Assessment in Mouse and Human Blood
title_full Utility of Platelet Endothelial Cell Adhesion Molecule 1 in the Platelet Activity Assessment in Mouse and Human Blood
title_fullStr Utility of Platelet Endothelial Cell Adhesion Molecule 1 in the Platelet Activity Assessment in Mouse and Human Blood
title_full_unstemmed Utility of Platelet Endothelial Cell Adhesion Molecule 1 in the Platelet Activity Assessment in Mouse and Human Blood
title_short Utility of Platelet Endothelial Cell Adhesion Molecule 1 in the Platelet Activity Assessment in Mouse and Human Blood
title_sort utility of platelet endothelial cell adhesion molecule 1 in the platelet activity assessment in mouse and human blood
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8431756/
https://www.ncbi.nlm.nih.gov/pubmed/34502520
http://dx.doi.org/10.3390/ijms22179611
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