Cargando…

Therapeutic Strategies for IVD Regeneration through Hyaluronan/SDF-1-Based Hydrogel and Intravenous Administration of MSCs

Intervertebral disc (IVD) degeneration involves a complex cascade of events, including degradation of the native extracellular matrix, loss of water content, and decreased cell numbers. Cell recruitment strategies for the IVD have been increasingly explored, aiming to recruit either endogenous or tr...

Descripción completa

Detalles Bibliográficos
Autores principales: Cunha, Carla, Leite Pereira, Catarina, Ferreira, Joana R., Ribeiro-Machado, Cláudia, Grad, Sibylle, Santos, Susana G., Gonçalves, Raquel M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8431759/
https://www.ncbi.nlm.nih.gov/pubmed/34502517
http://dx.doi.org/10.3390/ijms22179609
_version_ 1783751012946804736
author Cunha, Carla
Leite Pereira, Catarina
Ferreira, Joana R.
Ribeiro-Machado, Cláudia
Grad, Sibylle
Santos, Susana G.
Gonçalves, Raquel M.
author_facet Cunha, Carla
Leite Pereira, Catarina
Ferreira, Joana R.
Ribeiro-Machado, Cláudia
Grad, Sibylle
Santos, Susana G.
Gonçalves, Raquel M.
author_sort Cunha, Carla
collection PubMed
description Intervertebral disc (IVD) degeneration involves a complex cascade of events, including degradation of the native extracellular matrix, loss of water content, and decreased cell numbers. Cell recruitment strategies for the IVD have been increasingly explored, aiming to recruit either endogenous or transplanted cells. This study evaluates the IVD therapeutic potential of a chemoattractant delivery system (HAPSDF5) that combines a hyaluronan-based thermoreversible hydrogel (HAP) and the chemokine stromal cell derived factor-1 (SDF-1). HAPSDF5 was injected into the IVD and was combined with an intravenous injection of mesenchymal stem/stromal cells (MSCs) in a pre-clinical in vivo IVD lesion model. The local and systemic effects were evaluated two weeks after treatment. The hydrogel by itself (HAP) did not elicit any adverse effect, showing potential to be administrated by intradiscal injection. HAPSDF5 induced higher cell numbers, but no evidence of IVD regeneration was observed. MSCs systemic injection seemed to exert a role in IVD regeneration to some extent through a paracrine effect, but no synergies were observed when HAPSDF5 was combined with MSCs. Overall, this study shows that although the injection of chemoattractant hydrogels and MSC recruitment are feasible approaches for IVD, IVD regeneration using this strategy needs to be further explored before successful clinical translation.
format Online
Article
Text
id pubmed-8431759
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-84317592021-09-11 Therapeutic Strategies for IVD Regeneration through Hyaluronan/SDF-1-Based Hydrogel and Intravenous Administration of MSCs Cunha, Carla Leite Pereira, Catarina Ferreira, Joana R. Ribeiro-Machado, Cláudia Grad, Sibylle Santos, Susana G. Gonçalves, Raquel M. Int J Mol Sci Article Intervertebral disc (IVD) degeneration involves a complex cascade of events, including degradation of the native extracellular matrix, loss of water content, and decreased cell numbers. Cell recruitment strategies for the IVD have been increasingly explored, aiming to recruit either endogenous or transplanted cells. This study evaluates the IVD therapeutic potential of a chemoattractant delivery system (HAPSDF5) that combines a hyaluronan-based thermoreversible hydrogel (HAP) and the chemokine stromal cell derived factor-1 (SDF-1). HAPSDF5 was injected into the IVD and was combined with an intravenous injection of mesenchymal stem/stromal cells (MSCs) in a pre-clinical in vivo IVD lesion model. The local and systemic effects were evaluated two weeks after treatment. The hydrogel by itself (HAP) did not elicit any adverse effect, showing potential to be administrated by intradiscal injection. HAPSDF5 induced higher cell numbers, but no evidence of IVD regeneration was observed. MSCs systemic injection seemed to exert a role in IVD regeneration to some extent through a paracrine effect, but no synergies were observed when HAPSDF5 was combined with MSCs. Overall, this study shows that although the injection of chemoattractant hydrogels and MSC recruitment are feasible approaches for IVD, IVD regeneration using this strategy needs to be further explored before successful clinical translation. MDPI 2021-09-04 /pmc/articles/PMC8431759/ /pubmed/34502517 http://dx.doi.org/10.3390/ijms22179609 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Cunha, Carla
Leite Pereira, Catarina
Ferreira, Joana R.
Ribeiro-Machado, Cláudia
Grad, Sibylle
Santos, Susana G.
Gonçalves, Raquel M.
Therapeutic Strategies for IVD Regeneration through Hyaluronan/SDF-1-Based Hydrogel and Intravenous Administration of MSCs
title Therapeutic Strategies for IVD Regeneration through Hyaluronan/SDF-1-Based Hydrogel and Intravenous Administration of MSCs
title_full Therapeutic Strategies for IVD Regeneration through Hyaluronan/SDF-1-Based Hydrogel and Intravenous Administration of MSCs
title_fullStr Therapeutic Strategies for IVD Regeneration through Hyaluronan/SDF-1-Based Hydrogel and Intravenous Administration of MSCs
title_full_unstemmed Therapeutic Strategies for IVD Regeneration through Hyaluronan/SDF-1-Based Hydrogel and Intravenous Administration of MSCs
title_short Therapeutic Strategies for IVD Regeneration through Hyaluronan/SDF-1-Based Hydrogel and Intravenous Administration of MSCs
title_sort therapeutic strategies for ivd regeneration through hyaluronan/sdf-1-based hydrogel and intravenous administration of mscs
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8431759/
https://www.ncbi.nlm.nih.gov/pubmed/34502517
http://dx.doi.org/10.3390/ijms22179609
work_keys_str_mv AT cunhacarla therapeuticstrategiesforivdregenerationthroughhyaluronansdf1basedhydrogelandintravenousadministrationofmscs
AT leitepereiracatarina therapeuticstrategiesforivdregenerationthroughhyaluronansdf1basedhydrogelandintravenousadministrationofmscs
AT ferreirajoanar therapeuticstrategiesforivdregenerationthroughhyaluronansdf1basedhydrogelandintravenousadministrationofmscs
AT ribeiromachadoclaudia therapeuticstrategiesforivdregenerationthroughhyaluronansdf1basedhydrogelandintravenousadministrationofmscs
AT gradsibylle therapeuticstrategiesforivdregenerationthroughhyaluronansdf1basedhydrogelandintravenousadministrationofmscs
AT santossusanag therapeuticstrategiesforivdregenerationthroughhyaluronansdf1basedhydrogelandintravenousadministrationofmscs
AT goncalvesraquelm therapeuticstrategiesforivdregenerationthroughhyaluronansdf1basedhydrogelandintravenousadministrationofmscs