Cargando…

Impact of Polyethylene Glycol Functionalization of Graphene Oxide on Anticoagulation and Haemolytic Properties of Human Blood

Graphene oxide (GO) is one of the most explored nanomaterials in recent years. It has numerous biomedical applications as a nanomaterial including drug and gene delivery, contrast imaging, cancer treatment, etc. Since most of these applications need intravenous administration of graphene oxide and d...

Descripción completa

Detalles Bibliográficos
Autores principales: Kamenska, Trayana, Abrashev, Miroslav, Georgieva, Milena, Krasteva, Natalia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8432731/
https://www.ncbi.nlm.nih.gov/pubmed/34500942
http://dx.doi.org/10.3390/ma14174853
_version_ 1783751226831142912
author Kamenska, Trayana
Abrashev, Miroslav
Georgieva, Milena
Krasteva, Natalia
author_facet Kamenska, Trayana
Abrashev, Miroslav
Georgieva, Milena
Krasteva, Natalia
author_sort Kamenska, Trayana
collection PubMed
description Graphene oxide (GO) is one of the most explored nanomaterials in recent years. It has numerous biomedical applications as a nanomaterial including drug and gene delivery, contrast imaging, cancer treatment, etc. Since most of these applications need intravenous administration of graphene oxide and derivatives, the evaluation of their haemocompatibility is an essential preliminary step for any of the developed GO applications. Plentiful data show that functionalization of graphene oxide nanoparticles with polyethylene glycol (PEG) increases biocompatibility, thus allowing PEGylated GO to elicit less dramatic blood cell responses than their pristine counterparts. Therefore, in this work, we PEGylated graphene oxide nanoparticles and evaluated the effects of their PEGylation on the structure and function of human blood components, especially on the morphology and the haemolytic potential of red blood cells (RBCs). Further, we studied the effect of PEGylation on some blood coagulation factors, including plasma fibrinogen as well as on the activated partial thromboplastin (aPTT), prothrombin time (PT) and platelet aggregation. Our findings provide important information on the mechanisms through which PEGylation increases GO compatibility with human blood cells. These data are crucial for the molecular design and biomedical applications of PEGylated graphene oxide nanomaterials in the future.
format Online
Article
Text
id pubmed-8432731
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-84327312021-09-11 Impact of Polyethylene Glycol Functionalization of Graphene Oxide on Anticoagulation and Haemolytic Properties of Human Blood Kamenska, Trayana Abrashev, Miroslav Georgieva, Milena Krasteva, Natalia Materials (Basel) Article Graphene oxide (GO) is one of the most explored nanomaterials in recent years. It has numerous biomedical applications as a nanomaterial including drug and gene delivery, contrast imaging, cancer treatment, etc. Since most of these applications need intravenous administration of graphene oxide and derivatives, the evaluation of their haemocompatibility is an essential preliminary step for any of the developed GO applications. Plentiful data show that functionalization of graphene oxide nanoparticles with polyethylene glycol (PEG) increases biocompatibility, thus allowing PEGylated GO to elicit less dramatic blood cell responses than their pristine counterparts. Therefore, in this work, we PEGylated graphene oxide nanoparticles and evaluated the effects of their PEGylation on the structure and function of human blood components, especially on the morphology and the haemolytic potential of red blood cells (RBCs). Further, we studied the effect of PEGylation on some blood coagulation factors, including plasma fibrinogen as well as on the activated partial thromboplastin (aPTT), prothrombin time (PT) and platelet aggregation. Our findings provide important information on the mechanisms through which PEGylation increases GO compatibility with human blood cells. These data are crucial for the molecular design and biomedical applications of PEGylated graphene oxide nanomaterials in the future. MDPI 2021-08-26 /pmc/articles/PMC8432731/ /pubmed/34500942 http://dx.doi.org/10.3390/ma14174853 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kamenska, Trayana
Abrashev, Miroslav
Georgieva, Milena
Krasteva, Natalia
Impact of Polyethylene Glycol Functionalization of Graphene Oxide on Anticoagulation and Haemolytic Properties of Human Blood
title Impact of Polyethylene Glycol Functionalization of Graphene Oxide on Anticoagulation and Haemolytic Properties of Human Blood
title_full Impact of Polyethylene Glycol Functionalization of Graphene Oxide on Anticoagulation and Haemolytic Properties of Human Blood
title_fullStr Impact of Polyethylene Glycol Functionalization of Graphene Oxide on Anticoagulation and Haemolytic Properties of Human Blood
title_full_unstemmed Impact of Polyethylene Glycol Functionalization of Graphene Oxide on Anticoagulation and Haemolytic Properties of Human Blood
title_short Impact of Polyethylene Glycol Functionalization of Graphene Oxide on Anticoagulation and Haemolytic Properties of Human Blood
title_sort impact of polyethylene glycol functionalization of graphene oxide on anticoagulation and haemolytic properties of human blood
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8432731/
https://www.ncbi.nlm.nih.gov/pubmed/34500942
http://dx.doi.org/10.3390/ma14174853
work_keys_str_mv AT kamenskatrayana impactofpolyethyleneglycolfunctionalizationofgrapheneoxideonanticoagulationandhaemolyticpropertiesofhumanblood
AT abrashevmiroslav impactofpolyethyleneglycolfunctionalizationofgrapheneoxideonanticoagulationandhaemolyticpropertiesofhumanblood
AT georgievamilena impactofpolyethyleneglycolfunctionalizationofgrapheneoxideonanticoagulationandhaemolyticpropertiesofhumanblood
AT krastevanatalia impactofpolyethyleneglycolfunctionalizationofgrapheneoxideonanticoagulationandhaemolyticpropertiesofhumanblood