Cargando…

Probiotic based-diet effect on the immune response and induced stress in irradiated mass reared Ceratitis capitata males (Diptera: Tephritidae) destined for the release in the sterile insect technique programs

Ceratitis capitata (medfly) is one of the most devastating crop pests worldwide. The Sterile Insect Technique (SIT) is a control method that is based on the mass rearing of males, their sterilization, and release in the field. However, the effectiveness of the technique depends on the quality of the...

Descripción completa

Detalles Bibliográficos
Autores principales: Msaad Guerfali, Meriem, Charaabi, Kamel, Hamden, Haytham, Djobbi, Wafa, Fadhl, Salma, Mosbah, Amor, Cherif, Ameur
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8432743/
https://www.ncbi.nlm.nih.gov/pubmed/34506561
http://dx.doi.org/10.1371/journal.pone.0257097
_version_ 1783751228502573056
author Msaad Guerfali, Meriem
Charaabi, Kamel
Hamden, Haytham
Djobbi, Wafa
Fadhl, Salma
Mosbah, Amor
Cherif, Ameur
author_facet Msaad Guerfali, Meriem
Charaabi, Kamel
Hamden, Haytham
Djobbi, Wafa
Fadhl, Salma
Mosbah, Amor
Cherif, Ameur
author_sort Msaad Guerfali, Meriem
collection PubMed
description Ceratitis capitata (medfly) is one of the most devastating crop pests worldwide. The Sterile Insect Technique (SIT) is a control method that is based on the mass rearing of males, their sterilization, and release in the field. However, the effectiveness of the technique depends on the quality of the released males and their fitness. We previously isolated and selected a probiotic bacteria (Enterobacter sp.), from wild-caught medflies, according to criteria that improved biological quality traits of reared medfly males.We firstly evaluated the impact of the irradiation on the expression of different immune and stress genes in the medfly sterile males. Expression was measured at differents time points ranging from 0 to 168 h after irradiation to capture the response of genes with distinct temporal expression patterns. Then, we supplemented the larval diet with previously isolated Enterobacter sp.strain, live and autoclaved at various concentrations to see whether the probiotic treatments affect, through their protective role, the gene expression level, and quality traits. The irradiation had significant effect on the genes attacin, cecropin, PGPR-LC, hsp23, and hsp70 level expression. The expression of attacin and PGPR-LC was up-regulated while that of cecropin was down-regulated. Hsp genes showed decreased levels between 0 and 18 h to peak at 72 h. However, the supplementation of the probiotic strain, either live or autoclaved, was statistically significant only for attacingene. However, significant interaction time x probiotic was noticed for attacin, cecropin, hsp23 and hsp70. The probiotic treatments also improved the quality control parameters like pupal weight. From this work we can conclude that a consortium of parabiotics (autoclaved probiotics) treatment will be recommended in insectaries considering both the beneficial effects on mass reared insects and its general safety for insectary workers and for environment.
format Online
Article
Text
id pubmed-8432743
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-84327432021-09-11 Probiotic based-diet effect on the immune response and induced stress in irradiated mass reared Ceratitis capitata males (Diptera: Tephritidae) destined for the release in the sterile insect technique programs Msaad Guerfali, Meriem Charaabi, Kamel Hamden, Haytham Djobbi, Wafa Fadhl, Salma Mosbah, Amor Cherif, Ameur PLoS One Research Article Ceratitis capitata (medfly) is one of the most devastating crop pests worldwide. The Sterile Insect Technique (SIT) is a control method that is based on the mass rearing of males, their sterilization, and release in the field. However, the effectiveness of the technique depends on the quality of the released males and their fitness. We previously isolated and selected a probiotic bacteria (Enterobacter sp.), from wild-caught medflies, according to criteria that improved biological quality traits of reared medfly males.We firstly evaluated the impact of the irradiation on the expression of different immune and stress genes in the medfly sterile males. Expression was measured at differents time points ranging from 0 to 168 h after irradiation to capture the response of genes with distinct temporal expression patterns. Then, we supplemented the larval diet with previously isolated Enterobacter sp.strain, live and autoclaved at various concentrations to see whether the probiotic treatments affect, through their protective role, the gene expression level, and quality traits. The irradiation had significant effect on the genes attacin, cecropin, PGPR-LC, hsp23, and hsp70 level expression. The expression of attacin and PGPR-LC was up-regulated while that of cecropin was down-regulated. Hsp genes showed decreased levels between 0 and 18 h to peak at 72 h. However, the supplementation of the probiotic strain, either live or autoclaved, was statistically significant only for attacingene. However, significant interaction time x probiotic was noticed for attacin, cecropin, hsp23 and hsp70. The probiotic treatments also improved the quality control parameters like pupal weight. From this work we can conclude that a consortium of parabiotics (autoclaved probiotics) treatment will be recommended in insectaries considering both the beneficial effects on mass reared insects and its general safety for insectary workers and for environment. Public Library of Science 2021-09-10 /pmc/articles/PMC8432743/ /pubmed/34506561 http://dx.doi.org/10.1371/journal.pone.0257097 Text en © 2021 Msaad Guerfali et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Msaad Guerfali, Meriem
Charaabi, Kamel
Hamden, Haytham
Djobbi, Wafa
Fadhl, Salma
Mosbah, Amor
Cherif, Ameur
Probiotic based-diet effect on the immune response and induced stress in irradiated mass reared Ceratitis capitata males (Diptera: Tephritidae) destined for the release in the sterile insect technique programs
title Probiotic based-diet effect on the immune response and induced stress in irradiated mass reared Ceratitis capitata males (Diptera: Tephritidae) destined for the release in the sterile insect technique programs
title_full Probiotic based-diet effect on the immune response and induced stress in irradiated mass reared Ceratitis capitata males (Diptera: Tephritidae) destined for the release in the sterile insect technique programs
title_fullStr Probiotic based-diet effect on the immune response and induced stress in irradiated mass reared Ceratitis capitata males (Diptera: Tephritidae) destined for the release in the sterile insect technique programs
title_full_unstemmed Probiotic based-diet effect on the immune response and induced stress in irradiated mass reared Ceratitis capitata males (Diptera: Tephritidae) destined for the release in the sterile insect technique programs
title_short Probiotic based-diet effect on the immune response and induced stress in irradiated mass reared Ceratitis capitata males (Diptera: Tephritidae) destined for the release in the sterile insect technique programs
title_sort probiotic based-diet effect on the immune response and induced stress in irradiated mass reared ceratitis capitata males (diptera: tephritidae) destined for the release in the sterile insect technique programs
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8432743/
https://www.ncbi.nlm.nih.gov/pubmed/34506561
http://dx.doi.org/10.1371/journal.pone.0257097
work_keys_str_mv AT msaadguerfalimeriem probioticbaseddieteffectontheimmuneresponseandinducedstressinirradiatedmassrearedceratitiscapitatamalesdipteratephritidaedestinedforthereleaseinthesterileinsecttechniqueprograms
AT charaabikamel probioticbaseddieteffectontheimmuneresponseandinducedstressinirradiatedmassrearedceratitiscapitatamalesdipteratephritidaedestinedforthereleaseinthesterileinsecttechniqueprograms
AT hamdenhaytham probioticbaseddieteffectontheimmuneresponseandinducedstressinirradiatedmassrearedceratitiscapitatamalesdipteratephritidaedestinedforthereleaseinthesterileinsecttechniqueprograms
AT djobbiwafa probioticbaseddieteffectontheimmuneresponseandinducedstressinirradiatedmassrearedceratitiscapitatamalesdipteratephritidaedestinedforthereleaseinthesterileinsecttechniqueprograms
AT fadhlsalma probioticbaseddieteffectontheimmuneresponseandinducedstressinirradiatedmassrearedceratitiscapitatamalesdipteratephritidaedestinedforthereleaseinthesterileinsecttechniqueprograms
AT mosbahamor probioticbaseddieteffectontheimmuneresponseandinducedstressinirradiatedmassrearedceratitiscapitatamalesdipteratephritidaedestinedforthereleaseinthesterileinsecttechniqueprograms
AT cherifameur probioticbaseddieteffectontheimmuneresponseandinducedstressinirradiatedmassrearedceratitiscapitatamalesdipteratephritidaedestinedforthereleaseinthesterileinsecttechniqueprograms