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Fungus-originated glucanase and monooxygenase genes in creeping bent grass (Agrostis stolonifera L.)

Recent studies have revealed presence of fungus-originated genes in genomes of cool-season grasses, suggesting occurrence of multiple ancestral gene transfer events between the two distant lineages. The current article describes identification of glucanase-like and monooxygenase-like genes from cree...

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Autores principales: Watanabe, Yugo, Spangenberg, German C., Shinozuka, Hiroshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8432771/
https://www.ncbi.nlm.nih.gov/pubmed/34506557
http://dx.doi.org/10.1371/journal.pone.0257173
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author Watanabe, Yugo
Spangenberg, German C.
Shinozuka, Hiroshi
author_facet Watanabe, Yugo
Spangenberg, German C.
Shinozuka, Hiroshi
author_sort Watanabe, Yugo
collection PubMed
description Recent studies have revealed presence of fungus-originated genes in genomes of cool-season grasses, suggesting occurrence of multiple ancestral gene transfer events between the two distant lineages. The current article describes identification of glucanase-like and monooxygenase-like genes from creeping bent grass, as lateral gene transfer candidates. An in silico analysis suggested presence of the glucanase-like gene in Agrostis, Deyeuxia, and Polypogon genera, but not in other species belonging to the clade 1 of the Poeae tribe. Similarly, the monooxygenase-like gene was confined to Agrostis and Deyeuxia genera. A consistent result was obtained from PCR-based screening. The glucanase-like gene was revealed to be ubiquitously expressed in young seedlings of creeping bent grass. Although expression of the monooxygenase-like gene was suggested in plant tissues, the levels were considerably lower than those of the glucanase-like gene. A phylogenetic analysis revealed close relationships of the two genes between the corresponding genes in fungal endophyte species of the Epichloë genus, suggesting that the genes originated from the Epichloë lineage.
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spelling pubmed-84327712021-09-11 Fungus-originated glucanase and monooxygenase genes in creeping bent grass (Agrostis stolonifera L.) Watanabe, Yugo Spangenberg, German C. Shinozuka, Hiroshi PLoS One Research Article Recent studies have revealed presence of fungus-originated genes in genomes of cool-season grasses, suggesting occurrence of multiple ancestral gene transfer events between the two distant lineages. The current article describes identification of glucanase-like and monooxygenase-like genes from creeping bent grass, as lateral gene transfer candidates. An in silico analysis suggested presence of the glucanase-like gene in Agrostis, Deyeuxia, and Polypogon genera, but not in other species belonging to the clade 1 of the Poeae tribe. Similarly, the monooxygenase-like gene was confined to Agrostis and Deyeuxia genera. A consistent result was obtained from PCR-based screening. The glucanase-like gene was revealed to be ubiquitously expressed in young seedlings of creeping bent grass. Although expression of the monooxygenase-like gene was suggested in plant tissues, the levels were considerably lower than those of the glucanase-like gene. A phylogenetic analysis revealed close relationships of the two genes between the corresponding genes in fungal endophyte species of the Epichloë genus, suggesting that the genes originated from the Epichloë lineage. Public Library of Science 2021-09-10 /pmc/articles/PMC8432771/ /pubmed/34506557 http://dx.doi.org/10.1371/journal.pone.0257173 Text en © 2021 Watanabe et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Watanabe, Yugo
Spangenberg, German C.
Shinozuka, Hiroshi
Fungus-originated glucanase and monooxygenase genes in creeping bent grass (Agrostis stolonifera L.)
title Fungus-originated glucanase and monooxygenase genes in creeping bent grass (Agrostis stolonifera L.)
title_full Fungus-originated glucanase and monooxygenase genes in creeping bent grass (Agrostis stolonifera L.)
title_fullStr Fungus-originated glucanase and monooxygenase genes in creeping bent grass (Agrostis stolonifera L.)
title_full_unstemmed Fungus-originated glucanase and monooxygenase genes in creeping bent grass (Agrostis stolonifera L.)
title_short Fungus-originated glucanase and monooxygenase genes in creeping bent grass (Agrostis stolonifera L.)
title_sort fungus-originated glucanase and monooxygenase genes in creeping bent grass (agrostis stolonifera l.)
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8432771/
https://www.ncbi.nlm.nih.gov/pubmed/34506557
http://dx.doi.org/10.1371/journal.pone.0257173
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