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Visual naked-eye detection of SARS-CoV-2 RNA based on covalent organic framework capsules

The ongoing outbreak of coronavirus disease 2019 (COVID-19) caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has highlighted that new diagnosis technologies are crucial for controlling the spread of the disease. Especially in the resources-limit region, conveniently operate...

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Autores principales: Wang, Minghui, Lin, Yuxin, Lu, Jianyang, Sun, Zhaowei, Deng, Ying, Wang, Lei, Yi, Yongxiang, Li, Jinlong, Yang, Jie, Li, Genxi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8433056/
https://www.ncbi.nlm.nih.gov/pubmed/34539223
http://dx.doi.org/10.1016/j.cej.2021.132332
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author Wang, Minghui
Lin, Yuxin
Lu, Jianyang
Sun, Zhaowei
Deng, Ying
Wang, Lei
Yi, Yongxiang
Li, Jinlong
Yang, Jie
Li, Genxi
author_facet Wang, Minghui
Lin, Yuxin
Lu, Jianyang
Sun, Zhaowei
Deng, Ying
Wang, Lei
Yi, Yongxiang
Li, Jinlong
Yang, Jie
Li, Genxi
author_sort Wang, Minghui
collection PubMed
description The ongoing outbreak of coronavirus disease 2019 (COVID-19) caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has highlighted that new diagnosis technologies are crucial for controlling the spread of the disease. Especially in the resources-limit region, conveniently operated detection methods such as “naked-eye” detection are urgently required that no instrument is needed. Herein, we have designed a novel and facile strategy to fabricate covalent organic framework (COF) capsules, which can be utilized to establish a new colorimetric assay for naked-eye detection of SARS-CoV-2 RNA. Specifically, we employ the digestible ZIF-90 as the sacrificial template to prepare the hollow COF capsules for horseradish peroxidase (HRP) encapsulation. The fabricated COF capsules can provide an appropriate microenvironment for the enzyme molecules, which may improve the conformational freedom of enzymes, enhance the mass transfer, and endow the enzyme with high environmental resistance. With such design, the proposed assay exhibits outstanding analytical performance for the detection of SARS-CoV-2 RNA in the linear range from 5 pM to 50 nM with a detection limit of 0.28 pM which can go parallel to qTR-PCR analysis. Our method also possesses excellent selectivity and reproducibility. Moreover, this method can also be served to analyze the clinical samples, and can successfully differentiate COVID-19 patients from healthy people, suggesting the promising potential in clinical diagnosis.
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spelling pubmed-84330562021-09-13 Visual naked-eye detection of SARS-CoV-2 RNA based on covalent organic framework capsules Wang, Minghui Lin, Yuxin Lu, Jianyang Sun, Zhaowei Deng, Ying Wang, Lei Yi, Yongxiang Li, Jinlong Yang, Jie Li, Genxi Chem Eng J Article The ongoing outbreak of coronavirus disease 2019 (COVID-19) caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has highlighted that new diagnosis technologies are crucial for controlling the spread of the disease. Especially in the resources-limit region, conveniently operated detection methods such as “naked-eye” detection are urgently required that no instrument is needed. Herein, we have designed a novel and facile strategy to fabricate covalent organic framework (COF) capsules, which can be utilized to establish a new colorimetric assay for naked-eye detection of SARS-CoV-2 RNA. Specifically, we employ the digestible ZIF-90 as the sacrificial template to prepare the hollow COF capsules for horseradish peroxidase (HRP) encapsulation. The fabricated COF capsules can provide an appropriate microenvironment for the enzyme molecules, which may improve the conformational freedom of enzymes, enhance the mass transfer, and endow the enzyme with high environmental resistance. With such design, the proposed assay exhibits outstanding analytical performance for the detection of SARS-CoV-2 RNA in the linear range from 5 pM to 50 nM with a detection limit of 0.28 pM which can go parallel to qTR-PCR analysis. Our method also possesses excellent selectivity and reproducibility. Moreover, this method can also be served to analyze the clinical samples, and can successfully differentiate COVID-19 patients from healthy people, suggesting the promising potential in clinical diagnosis. Elsevier B.V. 2022-02-01 2021-09-11 /pmc/articles/PMC8433056/ /pubmed/34539223 http://dx.doi.org/10.1016/j.cej.2021.132332 Text en © 2021 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Wang, Minghui
Lin, Yuxin
Lu, Jianyang
Sun, Zhaowei
Deng, Ying
Wang, Lei
Yi, Yongxiang
Li, Jinlong
Yang, Jie
Li, Genxi
Visual naked-eye detection of SARS-CoV-2 RNA based on covalent organic framework capsules
title Visual naked-eye detection of SARS-CoV-2 RNA based on covalent organic framework capsules
title_full Visual naked-eye detection of SARS-CoV-2 RNA based on covalent organic framework capsules
title_fullStr Visual naked-eye detection of SARS-CoV-2 RNA based on covalent organic framework capsules
title_full_unstemmed Visual naked-eye detection of SARS-CoV-2 RNA based on covalent organic framework capsules
title_short Visual naked-eye detection of SARS-CoV-2 RNA based on covalent organic framework capsules
title_sort visual naked-eye detection of sars-cov-2 rna based on covalent organic framework capsules
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8433056/
https://www.ncbi.nlm.nih.gov/pubmed/34539223
http://dx.doi.org/10.1016/j.cej.2021.132332
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