Cargando…
E-DNA scaffold sensors and the reagentless, single-step, measurement of HIV-diagnostic antibodies in human serum
The multiplexed, point-of-care measurement of specific antibodies could improve the speed with which diseases are diagnosed and their treatment initiated. To this end, we are developing E-DNA scaffold sensors, which consist of a rigid, nucleic acid “scaffold” attached on one end to an electrode and...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8433188/ https://www.ncbi.nlm.nih.gov/pubmed/34567628 http://dx.doi.org/10.1038/s41378-019-0119-5 |
_version_ | 1783751325602807808 |
---|---|
author | Parolo, Claudio Greenwood, Ava S. Ogden, Nathan E. Kang, Di Hawes, Chase Ortega, Gabriel Arroyo-Currás, Netzahualcóyotl Plaxco, Kevin W. |
author_facet | Parolo, Claudio Greenwood, Ava S. Ogden, Nathan E. Kang, Di Hawes, Chase Ortega, Gabriel Arroyo-Currás, Netzahualcóyotl Plaxco, Kevin W. |
author_sort | Parolo, Claudio |
collection | PubMed |
description | The multiplexed, point-of-care measurement of specific antibodies could improve the speed with which diseases are diagnosed and their treatment initiated. To this end, we are developing E-DNA scaffold sensors, which consist of a rigid, nucleic acid “scaffold” attached on one end to an electrode and presenting both a redox reporter and an epitope on the other. In the absence of antibody, the reporter efficiently transfers electrons when interrogated electrochemically. Binding-induced steric hindrance limits movement, reducing electron transfer in a manner that is both easily measured and quantitatively related to target concentration. Previously we have used monoclonal antibodies to explore the analytical performance of E-DNA sensors, showing that they support the rapid, single-step, quantitative detection of multiple antibodies in small volume samples. Here, in contrast, we employ authentic human samples to better explore the platform’s clinical potential. Specifically, we developed E-DNA sensors targeting three HIV-specific antibodies and then compared the analytical and clinical performance of these against those of gold standard serological techniques. Doing so we find that, although the multistep amplification of an ELISA leads to a lower detection limits, the clinical sensitivity of ELISAs, E-DNA sensors and lateral-flow dipsticks are indistinguishable across our test set. It thus appears that, by merging the quantitation and multiplexing of ELISAs with the convenience and speed of dipsticks, E-DNA scaffold sensors could significantly improve on current serological practice. |
format | Online Article Text |
id | pubmed-8433188 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-84331882021-09-24 E-DNA scaffold sensors and the reagentless, single-step, measurement of HIV-diagnostic antibodies in human serum Parolo, Claudio Greenwood, Ava S. Ogden, Nathan E. Kang, Di Hawes, Chase Ortega, Gabriel Arroyo-Currás, Netzahualcóyotl Plaxco, Kevin W. Microsyst Nanoeng Article The multiplexed, point-of-care measurement of specific antibodies could improve the speed with which diseases are diagnosed and their treatment initiated. To this end, we are developing E-DNA scaffold sensors, which consist of a rigid, nucleic acid “scaffold” attached on one end to an electrode and presenting both a redox reporter and an epitope on the other. In the absence of antibody, the reporter efficiently transfers electrons when interrogated electrochemically. Binding-induced steric hindrance limits movement, reducing electron transfer in a manner that is both easily measured and quantitatively related to target concentration. Previously we have used monoclonal antibodies to explore the analytical performance of E-DNA sensors, showing that they support the rapid, single-step, quantitative detection of multiple antibodies in small volume samples. Here, in contrast, we employ authentic human samples to better explore the platform’s clinical potential. Specifically, we developed E-DNA sensors targeting three HIV-specific antibodies and then compared the analytical and clinical performance of these against those of gold standard serological techniques. Doing so we find that, although the multistep amplification of an ELISA leads to a lower detection limits, the clinical sensitivity of ELISAs, E-DNA sensors and lateral-flow dipsticks are indistinguishable across our test set. It thus appears that, by merging the quantitation and multiplexing of ELISAs with the convenience and speed of dipsticks, E-DNA scaffold sensors could significantly improve on current serological practice. Nature Publishing Group UK 2020-03-23 /pmc/articles/PMC8433188/ /pubmed/34567628 http://dx.doi.org/10.1038/s41378-019-0119-5 Text en © The Author(s) 2020 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Parolo, Claudio Greenwood, Ava S. Ogden, Nathan E. Kang, Di Hawes, Chase Ortega, Gabriel Arroyo-Currás, Netzahualcóyotl Plaxco, Kevin W. E-DNA scaffold sensors and the reagentless, single-step, measurement of HIV-diagnostic antibodies in human serum |
title | E-DNA scaffold sensors and the reagentless, single-step, measurement of HIV-diagnostic antibodies in human serum |
title_full | E-DNA scaffold sensors and the reagentless, single-step, measurement of HIV-diagnostic antibodies in human serum |
title_fullStr | E-DNA scaffold sensors and the reagentless, single-step, measurement of HIV-diagnostic antibodies in human serum |
title_full_unstemmed | E-DNA scaffold sensors and the reagentless, single-step, measurement of HIV-diagnostic antibodies in human serum |
title_short | E-DNA scaffold sensors and the reagentless, single-step, measurement of HIV-diagnostic antibodies in human serum |
title_sort | e-dna scaffold sensors and the reagentless, single-step, measurement of hiv-diagnostic antibodies in human serum |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8433188/ https://www.ncbi.nlm.nih.gov/pubmed/34567628 http://dx.doi.org/10.1038/s41378-019-0119-5 |
work_keys_str_mv | AT paroloclaudio ednascaffoldsensorsandthereagentlesssinglestepmeasurementofhivdiagnosticantibodiesinhumanserum AT greenwoodavas ednascaffoldsensorsandthereagentlesssinglestepmeasurementofhivdiagnosticantibodiesinhumanserum AT ogdennathane ednascaffoldsensorsandthereagentlesssinglestepmeasurementofhivdiagnosticantibodiesinhumanserum AT kangdi ednascaffoldsensorsandthereagentlesssinglestepmeasurementofhivdiagnosticantibodiesinhumanserum AT haweschase ednascaffoldsensorsandthereagentlesssinglestepmeasurementofhivdiagnosticantibodiesinhumanserum AT ortegagabriel ednascaffoldsensorsandthereagentlesssinglestepmeasurementofhivdiagnosticantibodiesinhumanserum AT arroyocurrasnetzahualcoyotl ednascaffoldsensorsandthereagentlesssinglestepmeasurementofhivdiagnosticantibodiesinhumanserum AT plaxcokevinw ednascaffoldsensorsandthereagentlesssinglestepmeasurementofhivdiagnosticantibodiesinhumanserum |