Cargando…
3D imaging of human organs with micrometer resolution - applied to the endocrine pancreas
The possibility to quantitatively study specific molecular/cellular features of complete human organs with preserved spatial 3D context would have widespread implications for pre-clinical and clinical medicine. Whereas optical 3D imaging approaches have experienced a formidable revolution, they have...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8433206/ https://www.ncbi.nlm.nih.gov/pubmed/34508173 http://dx.doi.org/10.1038/s42003-021-02589-x |
_version_ | 1783751329628291072 |
---|---|
author | Hahn, Max Nord, Christoffer Eriksson, Maria Morini, Federico Alanentalo, Tomas Korsgren, Olle Ahlgren, Ulf |
author_facet | Hahn, Max Nord, Christoffer Eriksson, Maria Morini, Federico Alanentalo, Tomas Korsgren, Olle Ahlgren, Ulf |
author_sort | Hahn, Max |
collection | PubMed |
description | The possibility to quantitatively study specific molecular/cellular features of complete human organs with preserved spatial 3D context would have widespread implications for pre-clinical and clinical medicine. Whereas optical 3D imaging approaches have experienced a formidable revolution, they have remained limited due to current incapacities in obtaining specific labelling within large tissue volumes. We present a simple approach enabling reconstruction of antibody labeled cells within entire human organs with preserved organ context. We demonstrate the utility of the approach by providing volumetric data and 3D distribution of hundreds of thousands of islets of Langerhans within the human pancreas. By assessments of pancreata from non-diabetic and type 2 diabetic individuals, we display previously unrecognized features of the human islet mass distribution and pathology. As such, this method may contribute not only in unraveling new information of the pancreatic anatomy/pathophysiology, but it may be translated to essentially any antibody marker or organ system. |
format | Online Article Text |
id | pubmed-8433206 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-84332062021-09-24 3D imaging of human organs with micrometer resolution - applied to the endocrine pancreas Hahn, Max Nord, Christoffer Eriksson, Maria Morini, Federico Alanentalo, Tomas Korsgren, Olle Ahlgren, Ulf Commun Biol Article The possibility to quantitatively study specific molecular/cellular features of complete human organs with preserved spatial 3D context would have widespread implications for pre-clinical and clinical medicine. Whereas optical 3D imaging approaches have experienced a formidable revolution, they have remained limited due to current incapacities in obtaining specific labelling within large tissue volumes. We present a simple approach enabling reconstruction of antibody labeled cells within entire human organs with preserved organ context. We demonstrate the utility of the approach by providing volumetric data and 3D distribution of hundreds of thousands of islets of Langerhans within the human pancreas. By assessments of pancreata from non-diabetic and type 2 diabetic individuals, we display previously unrecognized features of the human islet mass distribution and pathology. As such, this method may contribute not only in unraveling new information of the pancreatic anatomy/pathophysiology, but it may be translated to essentially any antibody marker or organ system. Nature Publishing Group UK 2021-09-10 /pmc/articles/PMC8433206/ /pubmed/34508173 http://dx.doi.org/10.1038/s42003-021-02589-x Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Hahn, Max Nord, Christoffer Eriksson, Maria Morini, Federico Alanentalo, Tomas Korsgren, Olle Ahlgren, Ulf 3D imaging of human organs with micrometer resolution - applied to the endocrine pancreas |
title | 3D imaging of human organs with micrometer resolution - applied to the endocrine pancreas |
title_full | 3D imaging of human organs with micrometer resolution - applied to the endocrine pancreas |
title_fullStr | 3D imaging of human organs with micrometer resolution - applied to the endocrine pancreas |
title_full_unstemmed | 3D imaging of human organs with micrometer resolution - applied to the endocrine pancreas |
title_short | 3D imaging of human organs with micrometer resolution - applied to the endocrine pancreas |
title_sort | 3d imaging of human organs with micrometer resolution - applied to the endocrine pancreas |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8433206/ https://www.ncbi.nlm.nih.gov/pubmed/34508173 http://dx.doi.org/10.1038/s42003-021-02589-x |
work_keys_str_mv | AT hahnmax 3dimagingofhumanorganswithmicrometerresolutionappliedtotheendocrinepancreas AT nordchristoffer 3dimagingofhumanorganswithmicrometerresolutionappliedtotheendocrinepancreas AT erikssonmaria 3dimagingofhumanorganswithmicrometerresolutionappliedtotheendocrinepancreas AT morinifederico 3dimagingofhumanorganswithmicrometerresolutionappliedtotheendocrinepancreas AT alanentalotomas 3dimagingofhumanorganswithmicrometerresolutionappliedtotheendocrinepancreas AT korsgrenolle 3dimagingofhumanorganswithmicrometerresolutionappliedtotheendocrinepancreas AT ahlgrenulf 3dimagingofhumanorganswithmicrometerresolutionappliedtotheendocrinepancreas |