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A microfluidic approach for synchronous and nondestructive study of the permeability of multiple oocytes
Investigation of oocyte membrane permeability plays a crucial role in fertility preservation, reproductive medicine, and reproductive pharmacology. However, the commonly used methods have disadvantages such as high time consumption, low efficiency, and cumbersome data processing. In addition, the de...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8433209/ https://www.ncbi.nlm.nih.gov/pubmed/34567666 http://dx.doi.org/10.1038/s41378-020-0160-4 |
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author | Chen, Zhongrong Memon, Kashan Cao, Yunxia Zhao, Gang |
author_facet | Chen, Zhongrong Memon, Kashan Cao, Yunxia Zhao, Gang |
author_sort | Chen, Zhongrong |
collection | PubMed |
description | Investigation of oocyte membrane permeability plays a crucial role in fertility preservation, reproductive medicine, and reproductive pharmacology. However, the commonly used methods have disadvantages such as high time consumption, low efficiency, and cumbersome data processing. In addition, the developmental potential of oocytes after measurement has not been fully validated in previous studies. Moreover, oocytes can only maintain their best status in vitro within a very limited time. To address these limitations, we developed a novel multichannel microfluidic chip with newly designed micropillars that provide feasible and repeatable oocyte capture. The osmotic responses of three oocytes at different or the same cryoprotectant (CPA) concentrations were measured simultaneously, which greatly improved the measurement efficiency. Importantly, the CPA concentration dependence of mouse oocyte membrane permeability was found. Moreover, a neural network algorithm was employed to improve the efficiency and accuracy of data processing. Furthermore, analysis of fertilization and embryo transfer after perfusion indicated that the microfluidic approach does not damage the developmental potential of oocytes. In brief, we report a new method based on a multichannel microfluidic chip that enables synchronous and nondestructive measurement of the permeability of multiple oocytes. |
format | Online Article Text |
id | pubmed-8433209 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-84332092021-09-24 A microfluidic approach for synchronous and nondestructive study of the permeability of multiple oocytes Chen, Zhongrong Memon, Kashan Cao, Yunxia Zhao, Gang Microsyst Nanoeng Article Investigation of oocyte membrane permeability plays a crucial role in fertility preservation, reproductive medicine, and reproductive pharmacology. However, the commonly used methods have disadvantages such as high time consumption, low efficiency, and cumbersome data processing. In addition, the developmental potential of oocytes after measurement has not been fully validated in previous studies. Moreover, oocytes can only maintain their best status in vitro within a very limited time. To address these limitations, we developed a novel multichannel microfluidic chip with newly designed micropillars that provide feasible and repeatable oocyte capture. The osmotic responses of three oocytes at different or the same cryoprotectant (CPA) concentrations were measured simultaneously, which greatly improved the measurement efficiency. Importantly, the CPA concentration dependence of mouse oocyte membrane permeability was found. Moreover, a neural network algorithm was employed to improve the efficiency and accuracy of data processing. Furthermore, analysis of fertilization and embryo transfer after perfusion indicated that the microfluidic approach does not damage the developmental potential of oocytes. In brief, we report a new method based on a multichannel microfluidic chip that enables synchronous and nondestructive measurement of the permeability of multiple oocytes. Nature Publishing Group UK 2020-07-27 /pmc/articles/PMC8433209/ /pubmed/34567666 http://dx.doi.org/10.1038/s41378-020-0160-4 Text en © The Author(s) 2020 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Chen, Zhongrong Memon, Kashan Cao, Yunxia Zhao, Gang A microfluidic approach for synchronous and nondestructive study of the permeability of multiple oocytes |
title | A microfluidic approach for synchronous and nondestructive study of the permeability of multiple oocytes |
title_full | A microfluidic approach for synchronous and nondestructive study of the permeability of multiple oocytes |
title_fullStr | A microfluidic approach for synchronous and nondestructive study of the permeability of multiple oocytes |
title_full_unstemmed | A microfluidic approach for synchronous and nondestructive study of the permeability of multiple oocytes |
title_short | A microfluidic approach for synchronous and nondestructive study of the permeability of multiple oocytes |
title_sort | microfluidic approach for synchronous and nondestructive study of the permeability of multiple oocytes |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8433209/ https://www.ncbi.nlm.nih.gov/pubmed/34567666 http://dx.doi.org/10.1038/s41378-020-0160-4 |
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