Manipulation of TAD reorganization by chemical-dependent genome linking

Reorganization of topologically associated domain (TAD) is considered to be a novel mechanism for cell fate transitions. Here, we present a protocol to manipulate TAD via abscisic acid (ABA)-dependent genome linking. We use this protocol to merge two adjacent TADs and evaluate the influence on cell...

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Detalles Bibliográficos
Autores principales: Wang, Jia, Ma, Qian, Fang, Peihang, Tian, Qi, Yu, Haopeng, Sun, Junyi, Ding, Junjun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8433250/
https://www.ncbi.nlm.nih.gov/pubmed/34527956
http://dx.doi.org/10.1016/j.xpro.2021.100799
Descripción
Sumario:Reorganization of topologically associated domain (TAD) is considered to be a novel mechanism for cell fate transitions. Here, we present a protocol to manipulate TAD via abscisic acid (ABA)-dependent genome linking. We use this protocol to merge two adjacent TADs and evaluate the influence on cell fate transitions. The advantages are that the manipulation does not change the genome and is reversible by withdrawing ABA. The major challenge is how to select linking loci for efficient TAD reorganization. For complete details on the use and execution of this protocol, please refer to Wang et al. (2021).

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