Manipulation of TAD reorganization by chemical-dependent genome linking

Reorganization of topologically associated domain (TAD) is considered to be a novel mechanism for cell fate transitions. Here, we present a protocol to manipulate TAD via abscisic acid (ABA)-dependent genome linking. We use this protocol to merge two adjacent TADs and evaluate the influence on cell...

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Detalles Bibliográficos
Autores principales: Wang, Jia, Ma, Qian, Fang, Peihang, Tian, Qi, Yu, Haopeng, Sun, Junyi, Ding, Junjun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8433250/
https://www.ncbi.nlm.nih.gov/pubmed/34527956
http://dx.doi.org/10.1016/j.xpro.2021.100799
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author Wang, Jia
Ma, Qian
Fang, Peihang
Tian, Qi
Yu, Haopeng
Sun, Junyi
Ding, Junjun
author_facet Wang, Jia
Ma, Qian
Fang, Peihang
Tian, Qi
Yu, Haopeng
Sun, Junyi
Ding, Junjun
author_sort Wang, Jia
collection PubMed
description Reorganization of topologically associated domain (TAD) is considered to be a novel mechanism for cell fate transitions. Here, we present a protocol to manipulate TAD via abscisic acid (ABA)-dependent genome linking. We use this protocol to merge two adjacent TADs and evaluate the influence on cell fate transitions. The advantages are that the manipulation does not change the genome and is reversible by withdrawing ABA. The major challenge is how to select linking loci for efficient TAD reorganization. For complete details on the use and execution of this protocol, please refer to Wang et al. (2021).
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spelling pubmed-84332502021-09-14 Manipulation of TAD reorganization by chemical-dependent genome linking Wang, Jia Ma, Qian Fang, Peihang Tian, Qi Yu, Haopeng Sun, Junyi Ding, Junjun STAR Protoc Protocol Reorganization of topologically associated domain (TAD) is considered to be a novel mechanism for cell fate transitions. Here, we present a protocol to manipulate TAD via abscisic acid (ABA)-dependent genome linking. We use this protocol to merge two adjacent TADs and evaluate the influence on cell fate transitions. The advantages are that the manipulation does not change the genome and is reversible by withdrawing ABA. The major challenge is how to select linking loci for efficient TAD reorganization. For complete details on the use and execution of this protocol, please refer to Wang et al. (2021). Elsevier 2021-09-08 /pmc/articles/PMC8433250/ /pubmed/34527956 http://dx.doi.org/10.1016/j.xpro.2021.100799 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Wang, Jia
Ma, Qian
Fang, Peihang
Tian, Qi
Yu, Haopeng
Sun, Junyi
Ding, Junjun
Manipulation of TAD reorganization by chemical-dependent genome linking
title Manipulation of TAD reorganization by chemical-dependent genome linking
title_full Manipulation of TAD reorganization by chemical-dependent genome linking
title_fullStr Manipulation of TAD reorganization by chemical-dependent genome linking
title_full_unstemmed Manipulation of TAD reorganization by chemical-dependent genome linking
title_short Manipulation of TAD reorganization by chemical-dependent genome linking
title_sort manipulation of tad reorganization by chemical-dependent genome linking
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8433250/
https://www.ncbi.nlm.nih.gov/pubmed/34527956
http://dx.doi.org/10.1016/j.xpro.2021.100799
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