Detection of homolog-independent meiotic DNA repair events in C. elegans with the intersister/intrachromatid repair assay

Accurate repair of DNA double-strand breaks (DSBs) in developing germ cells is critical to promote proper chromosome segregation and to maintain genome integrity. To directly detect homolog-independent (intersister/intrachromatid) meiotic DSB repair, we exploited the genetics and germline physiology...

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Detalles Bibliográficos
Autores principales: Toraason, Erik, Glover, Marissa, Horacek, Anna, Libuda, Diana E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8433254/
https://www.ncbi.nlm.nih.gov/pubmed/34527958
http://dx.doi.org/10.1016/j.xpro.2021.100801
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author Toraason, Erik
Glover, Marissa
Horacek, Anna
Libuda, Diana E.
author_facet Toraason, Erik
Glover, Marissa
Horacek, Anna
Libuda, Diana E.
author_sort Toraason, Erik
collection PubMed
description Accurate repair of DNA double-strand breaks (DSBs) in developing germ cells is critical to promote proper chromosome segregation and to maintain genome integrity. To directly detect homolog-independent (intersister/intrachromatid) meiotic DSB repair, we exploited the genetics and germline physiology of C. elegans to (1) induce a single DSB in nuclei across discrete stages of meiotic prophase I; (2) detect repair of that DSB as a homolog-independent crossover or noncrossover; and (3) sequence the resultant product to assess mechanisms of recombination. For complete details on the use and execution of this protocol, please refer to Toraason et al. (2021).
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spelling pubmed-84332542021-09-14 Detection of homolog-independent meiotic DNA repair events in C. elegans with the intersister/intrachromatid repair assay Toraason, Erik Glover, Marissa Horacek, Anna Libuda, Diana E. STAR Protoc Protocol Accurate repair of DNA double-strand breaks (DSBs) in developing germ cells is critical to promote proper chromosome segregation and to maintain genome integrity. To directly detect homolog-independent (intersister/intrachromatid) meiotic DSB repair, we exploited the genetics and germline physiology of C. elegans to (1) induce a single DSB in nuclei across discrete stages of meiotic prophase I; (2) detect repair of that DSB as a homolog-independent crossover or noncrossover; and (3) sequence the resultant product to assess mechanisms of recombination. For complete details on the use and execution of this protocol, please refer to Toraason et al. (2021). Elsevier 2021-09-08 /pmc/articles/PMC8433254/ /pubmed/34527958 http://dx.doi.org/10.1016/j.xpro.2021.100801 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Toraason, Erik
Glover, Marissa
Horacek, Anna
Libuda, Diana E.
Detection of homolog-independent meiotic DNA repair events in C. elegans with the intersister/intrachromatid repair assay
title Detection of homolog-independent meiotic DNA repair events in C. elegans with the intersister/intrachromatid repair assay
title_full Detection of homolog-independent meiotic DNA repair events in C. elegans with the intersister/intrachromatid repair assay
title_fullStr Detection of homolog-independent meiotic DNA repair events in C. elegans with the intersister/intrachromatid repair assay
title_full_unstemmed Detection of homolog-independent meiotic DNA repair events in C. elegans with the intersister/intrachromatid repair assay
title_short Detection of homolog-independent meiotic DNA repair events in C. elegans with the intersister/intrachromatid repair assay
title_sort detection of homolog-independent meiotic dna repair events in c. elegans with the intersister/intrachromatid repair assay
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8433254/
https://www.ncbi.nlm.nih.gov/pubmed/34527958
http://dx.doi.org/10.1016/j.xpro.2021.100801
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