Purification of GFP-tagged nuclei from frozen livers of INTACT mice for RNA- and ATAC-sequencing

Isolation of nuclei tagged in specific cell types (INTACT) allows for stress-free and high-throughput analyses of cellular subpopulations. Here, we present an improved protocol for isolation of pure and high-quality GFP-labeled nuclei from frozen livers of INTACT mice, as well as protocols for downs...

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Detalles Bibliográficos
Autores principales: Loft, Anne, Herzig, Stephan, Schmidt, Søren Fisker
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8433282/
https://www.ncbi.nlm.nih.gov/pubmed/34527961
http://dx.doi.org/10.1016/j.xpro.2021.100805

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8433282/
https://www.ncbi.nlm.nih.gov/pubmed/34527961
http://dx.doi.org/10.1016/j.xpro.2021.100805

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