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Purification of GFP-tagged nuclei from frozen livers of INTACT mice for RNA- and ATAC-sequencing

Isolation of nuclei tagged in specific cell types (INTACT) allows for stress-free and high-throughput analyses of cellular subpopulations. Here, we present an improved protocol for isolation of pure and high-quality GFP-labeled nuclei from frozen livers of INTACT mice, as well as protocols for downs...

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Detalles Bibliográficos
Autores principales:	Loft, Anne, Herzig, Stephan, Schmidt, Søren Fisker
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2021
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8433282/ https://www.ncbi.nlm.nih.gov/pubmed/34527961 http://dx.doi.org/10.1016/j.xpro.2021.100805

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