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In-yeast reconstruction of the African swine fever virus genome isolated from clinical samples
This protocol describes a synthetic genomics pipeline to clone and engineer the entire 190-kbp genome of the African swine fever virus (ASFV) genotype II in yeast using transformation-associated recombination cloning. The viral genome was cloned using DNA directly extracted from a clinical sample. I...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8433286/ https://www.ncbi.nlm.nih.gov/pubmed/34527959 http://dx.doi.org/10.1016/j.xpro.2021.100803 |