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In-yeast reconstruction of the African swine fever virus genome isolated from clinical samples

This protocol describes a synthetic genomics pipeline to clone and engineer the entire 190-kbp genome of the African swine fever virus (ASFV) genotype II in yeast using transformation-associated recombination cloning. The viral genome was cloned using DNA directly extracted from a clinical sample. I...

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Detalles Bibliográficos
Autores principales:	Labroussaa, Fabien, Mehinagic, Kemal, Cippa, Valentina, Liniger, Matthias, Akarsu, Hatice, Ruggli, Nicolas, Jores, Joerg
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2021
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8433286/ https://www.ncbi.nlm.nih.gov/pubmed/34527959 http://dx.doi.org/10.1016/j.xpro.2021.100803

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