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The Collagen Origin Influences the Degradation Kinetics of Guided Bone Regeneration Membranes

Collagen membranes are currently the most widely used membranes for guided bone regeneration; however, their rapid degradation kinetics means that the barrier function may not remain for enough time to permit tissue regeneration to happen. The origin of collagen may have an important effect on the r...

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Autores principales: Vallecillo-Rivas, Marta, Toledano-Osorio, Manuel, Vallecillo, Cristina, Toledano, Manuel, Osorio, Raquel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8433692/
https://www.ncbi.nlm.nih.gov/pubmed/34503047
http://dx.doi.org/10.3390/polym13173007
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author Vallecillo-Rivas, Marta
Toledano-Osorio, Manuel
Vallecillo, Cristina
Toledano, Manuel
Osorio, Raquel
author_facet Vallecillo-Rivas, Marta
Toledano-Osorio, Manuel
Vallecillo, Cristina
Toledano, Manuel
Osorio, Raquel
author_sort Vallecillo-Rivas, Marta
collection PubMed
description Collagen membranes are currently the most widely used membranes for guided bone regeneration; however, their rapid degradation kinetics means that the barrier function may not remain for enough time to permit tissue regeneration to happen. The origin of collagen may have an important effect on the resistance to degradation. The aim of this study was to investigate the biodegradation pattern of five collagen membranes from different origins: Biocollagen, Heart, Evolution X-fine, CopiOs and Parasorb Resodont. Membranes samples were submitted to different degradation tests: (1) hydrolytic degradation in phosphate buffer saline solution, (2) bacterial collagenase from Clostridium histolyticum solution, and (3) enzyme resistance using a 0.25% porcine trypsin solution. Immersion periods from 1 up to 50 days were performed. At each time point, thickness and weight measurements were performed with a digital caliper and an analytic microbalance, respectively. ANOVA and Student–Newman–Keuls tests were used for comparisons (p < 0.05). Differences between time-points within the same membranes and solutions were assessed by pair-wise comparisons (p < 0.001). The Evolution X-fine collagen membrane from porcine pericardium attained the highest resistance to all of the degradation tests. Biocollagen and Parasorb Resodont, both from equine origin, experienced the greatest degradation when immersed in PBS, trypsin and C. histolyticum during challenge tests. The bacterial collagenase solution was shown to be the most aggressive testing method.
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spelling pubmed-84336922021-09-12 The Collagen Origin Influences the Degradation Kinetics of Guided Bone Regeneration Membranes Vallecillo-Rivas, Marta Toledano-Osorio, Manuel Vallecillo, Cristina Toledano, Manuel Osorio, Raquel Polymers (Basel) Article Collagen membranes are currently the most widely used membranes for guided bone regeneration; however, their rapid degradation kinetics means that the barrier function may not remain for enough time to permit tissue regeneration to happen. The origin of collagen may have an important effect on the resistance to degradation. The aim of this study was to investigate the biodegradation pattern of five collagen membranes from different origins: Biocollagen, Heart, Evolution X-fine, CopiOs and Parasorb Resodont. Membranes samples were submitted to different degradation tests: (1) hydrolytic degradation in phosphate buffer saline solution, (2) bacterial collagenase from Clostridium histolyticum solution, and (3) enzyme resistance using a 0.25% porcine trypsin solution. Immersion periods from 1 up to 50 days were performed. At each time point, thickness and weight measurements were performed with a digital caliper and an analytic microbalance, respectively. ANOVA and Student–Newman–Keuls tests were used for comparisons (p < 0.05). Differences between time-points within the same membranes and solutions were assessed by pair-wise comparisons (p < 0.001). The Evolution X-fine collagen membrane from porcine pericardium attained the highest resistance to all of the degradation tests. Biocollagen and Parasorb Resodont, both from equine origin, experienced the greatest degradation when immersed in PBS, trypsin and C. histolyticum during challenge tests. The bacterial collagenase solution was shown to be the most aggressive testing method. MDPI 2021-09-05 /pmc/articles/PMC8433692/ /pubmed/34503047 http://dx.doi.org/10.3390/polym13173007 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Vallecillo-Rivas, Marta
Toledano-Osorio, Manuel
Vallecillo, Cristina
Toledano, Manuel
Osorio, Raquel
The Collagen Origin Influences the Degradation Kinetics of Guided Bone Regeneration Membranes
title The Collagen Origin Influences the Degradation Kinetics of Guided Bone Regeneration Membranes
title_full The Collagen Origin Influences the Degradation Kinetics of Guided Bone Regeneration Membranes
title_fullStr The Collagen Origin Influences the Degradation Kinetics of Guided Bone Regeneration Membranes
title_full_unstemmed The Collagen Origin Influences the Degradation Kinetics of Guided Bone Regeneration Membranes
title_short The Collagen Origin Influences the Degradation Kinetics of Guided Bone Regeneration Membranes
title_sort collagen origin influences the degradation kinetics of guided bone regeneration membranes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8433692/
https://www.ncbi.nlm.nih.gov/pubmed/34503047
http://dx.doi.org/10.3390/polym13173007
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