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Functional Adhesion of Pectin Biopolymers to the Lung Visceral Pleura

Pleural injuries and the associated “air leak” are the most common complications after pulmonary surgery. Air leaks are the primary reason for prolonged chest tube use and increased hospital length of stay. Pectin, a plant-derived heteropolysaccharide, has been shown to be an air-tight sealant of pu...

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Autores principales: Zheng, Yifan, Pierce, Aidan F., Wagner, Willi L., Khalil, Hassan A., Chen, Zi, Servais, Andrew B., Ackermann, Maximilian, Mentzer, Steven J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8433721/
https://www.ncbi.nlm.nih.gov/pubmed/34503016
http://dx.doi.org/10.3390/polym13172976
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author Zheng, Yifan
Pierce, Aidan F.
Wagner, Willi L.
Khalil, Hassan A.
Chen, Zi
Servais, Andrew B.
Ackermann, Maximilian
Mentzer, Steven J.
author_facet Zheng, Yifan
Pierce, Aidan F.
Wagner, Willi L.
Khalil, Hassan A.
Chen, Zi
Servais, Andrew B.
Ackermann, Maximilian
Mentzer, Steven J.
author_sort Zheng, Yifan
collection PubMed
description Pleural injuries and the associated “air leak” are the most common complications after pulmonary surgery. Air leaks are the primary reason for prolonged chest tube use and increased hospital length of stay. Pectin, a plant-derived heteropolysaccharide, has been shown to be an air-tight sealant of pulmonary air leaks. Here, we investigate the morphologic and mechanical properties of pectin adhesion to the visceral pleural surface of the lung. After the application of high-methoxyl citrus pectin films to the murine lung, we used scanning electron microscopy to demonstrate intimate binding to the lung surface. To quantitatively assess pectin adhesion to the pleural surface, we used a custom adhesion test with force, distance, and time recordings. These assays demonstrated that pectin–glycocalyceal tensile adhesive strength was greater than nanocellulose fiber films or pressure-sensitive adhesives (p < 0.001). Simultaneous videomicroscopy recordings demonstrated that pectin–glycocalyceal adhesion was also stronger than the submesothelial connective tissue as avulsed surface remnants were visualized on the separated pectin films. Finally, pleural abrasion and hyaluronidase enzyme digestion confirmed that pectin binding was dependent on the pleural glycocalyx (p < 0.001). The results indicate that high methoxyl citrus pectin is a promising sealant for the treatment of pleural lung injuries.
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spelling pubmed-84337212021-09-12 Functional Adhesion of Pectin Biopolymers to the Lung Visceral Pleura Zheng, Yifan Pierce, Aidan F. Wagner, Willi L. Khalil, Hassan A. Chen, Zi Servais, Andrew B. Ackermann, Maximilian Mentzer, Steven J. Polymers (Basel) Article Pleural injuries and the associated “air leak” are the most common complications after pulmonary surgery. Air leaks are the primary reason for prolonged chest tube use and increased hospital length of stay. Pectin, a plant-derived heteropolysaccharide, has been shown to be an air-tight sealant of pulmonary air leaks. Here, we investigate the morphologic and mechanical properties of pectin adhesion to the visceral pleural surface of the lung. After the application of high-methoxyl citrus pectin films to the murine lung, we used scanning electron microscopy to demonstrate intimate binding to the lung surface. To quantitatively assess pectin adhesion to the pleural surface, we used a custom adhesion test with force, distance, and time recordings. These assays demonstrated that pectin–glycocalyceal tensile adhesive strength was greater than nanocellulose fiber films or pressure-sensitive adhesives (p < 0.001). Simultaneous videomicroscopy recordings demonstrated that pectin–glycocalyceal adhesion was also stronger than the submesothelial connective tissue as avulsed surface remnants were visualized on the separated pectin films. Finally, pleural abrasion and hyaluronidase enzyme digestion confirmed that pectin binding was dependent on the pleural glycocalyx (p < 0.001). The results indicate that high methoxyl citrus pectin is a promising sealant for the treatment of pleural lung injuries. MDPI 2021-09-02 /pmc/articles/PMC8433721/ /pubmed/34503016 http://dx.doi.org/10.3390/polym13172976 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zheng, Yifan
Pierce, Aidan F.
Wagner, Willi L.
Khalil, Hassan A.
Chen, Zi
Servais, Andrew B.
Ackermann, Maximilian
Mentzer, Steven J.
Functional Adhesion of Pectin Biopolymers to the Lung Visceral Pleura
title Functional Adhesion of Pectin Biopolymers to the Lung Visceral Pleura
title_full Functional Adhesion of Pectin Biopolymers to the Lung Visceral Pleura
title_fullStr Functional Adhesion of Pectin Biopolymers to the Lung Visceral Pleura
title_full_unstemmed Functional Adhesion of Pectin Biopolymers to the Lung Visceral Pleura
title_short Functional Adhesion of Pectin Biopolymers to the Lung Visceral Pleura
title_sort functional adhesion of pectin biopolymers to the lung visceral pleura
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8433721/
https://www.ncbi.nlm.nih.gov/pubmed/34503016
http://dx.doi.org/10.3390/polym13172976
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