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The Expression of Allele Changes in NLRP3 (rs35829419) and IL-1β (+3954) Gene Polymorphisms in Periodontitis and Coronary Artery Disease

Background: Inflammasomes have been shown to play a pivotal role in periodontal disease pathogenesis. However, their role in periodontitis subjects with coronary heart disease remains unclear. This study aimed to obtain the expression of NLRP3 (rs35829419) and IL-1β (+3954) gene polymorphisms in the...

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Autores principales: Mahendra, Jaideep, Rao, Abirami Nayaki, Mahendra, Little, Sayed, Mohammed E., Mugri, Maryam H., Balaji, Thodur Madapusi, Varadarajan, Saranya, Jagannathan, Raghunathan, Srinivasan, Sruthi, Baeshen, Hosam Ali, Mathew, Reji, Patil, Shankargouda
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8433916/
https://www.ncbi.nlm.nih.gov/pubmed/34501201
http://dx.doi.org/10.3390/ma14175103
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author Mahendra, Jaideep
Rao, Abirami Nayaki
Mahendra, Little
Sayed, Mohammed E.
Mugri, Maryam H.
Balaji, Thodur Madapusi
Varadarajan, Saranya
Jagannathan, Raghunathan
Srinivasan, Sruthi
Baeshen, Hosam Ali
Mathew, Reji
Patil, Shankargouda
author_facet Mahendra, Jaideep
Rao, Abirami Nayaki
Mahendra, Little
Sayed, Mohammed E.
Mugri, Maryam H.
Balaji, Thodur Madapusi
Varadarajan, Saranya
Jagannathan, Raghunathan
Srinivasan, Sruthi
Baeshen, Hosam Ali
Mathew, Reji
Patil, Shankargouda
author_sort Mahendra, Jaideep
collection PubMed
description Background: Inflammasomes have been shown to play a pivotal role in periodontal disease pathogenesis. However, their role in periodontitis subjects with coronary heart disease remains unclear. This study aimed to obtain the expression of NLRP3 (rs35829419) and IL-1β (+3954) gene polymorphisms in the subgingival plaque and blood samples of generalized periodontitis (GP) subjects with and without coronary heart disease (CHD). Methods: A total of 70 subjects were grouped into two; GP and GP with CHD. Demographic variables and periodontal and cardiac parameters were recorded from both the groups. Subgingival plaque and blood samples were obtained from both the groups and were further subjected to the identification of NLRP3 (rs35829419) and IL-1β (+3954) expression and allele change using a conventional polymerase chain reaction (PCR) and gene sequencing (Sanger’s method). Results: Amongst the demographic variables, age and monthly income were statistically significant between the two groups. Plaque index (PI), clinical attachment level (CAL), high-density lipoprotein (HDL), and low density-lipoprotein (LDL) exhibited statistically significant levels between the two groups. The NLRP3 (rs35829419) and IL-1β (+3954) genes showed a statistically significant association with allele change (frequency) among the groups. The general comparison of all the parameters with the allele change of NLRP3 (rs35829419) and IL-1β (+3954) in the subgingival plaque and blood samples showed statistically significant associations among the two groups. Conclusion: The present study highlighted an allele change in IL-1β (+3954) gene polymorphisms which may play an important role in the pathogenesis of periodontitis and coronary heart disease.
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spelling pubmed-84339162021-09-12 The Expression of Allele Changes in NLRP3 (rs35829419) and IL-1β (+3954) Gene Polymorphisms in Periodontitis and Coronary Artery Disease Mahendra, Jaideep Rao, Abirami Nayaki Mahendra, Little Sayed, Mohammed E. Mugri, Maryam H. Balaji, Thodur Madapusi Varadarajan, Saranya Jagannathan, Raghunathan Srinivasan, Sruthi Baeshen, Hosam Ali Mathew, Reji Patil, Shankargouda Materials (Basel) Article Background: Inflammasomes have been shown to play a pivotal role in periodontal disease pathogenesis. However, their role in periodontitis subjects with coronary heart disease remains unclear. This study aimed to obtain the expression of NLRP3 (rs35829419) and IL-1β (+3954) gene polymorphisms in the subgingival plaque and blood samples of generalized periodontitis (GP) subjects with and without coronary heart disease (CHD). Methods: A total of 70 subjects were grouped into two; GP and GP with CHD. Demographic variables and periodontal and cardiac parameters were recorded from both the groups. Subgingival plaque and blood samples were obtained from both the groups and were further subjected to the identification of NLRP3 (rs35829419) and IL-1β (+3954) expression and allele change using a conventional polymerase chain reaction (PCR) and gene sequencing (Sanger’s method). Results: Amongst the demographic variables, age and monthly income were statistically significant between the two groups. Plaque index (PI), clinical attachment level (CAL), high-density lipoprotein (HDL), and low density-lipoprotein (LDL) exhibited statistically significant levels between the two groups. The NLRP3 (rs35829419) and IL-1β (+3954) genes showed a statistically significant association with allele change (frequency) among the groups. The general comparison of all the parameters with the allele change of NLRP3 (rs35829419) and IL-1β (+3954) in the subgingival plaque and blood samples showed statistically significant associations among the two groups. Conclusion: The present study highlighted an allele change in IL-1β (+3954) gene polymorphisms which may play an important role in the pathogenesis of periodontitis and coronary heart disease. MDPI 2021-09-06 /pmc/articles/PMC8433916/ /pubmed/34501201 http://dx.doi.org/10.3390/ma14175103 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Mahendra, Jaideep
Rao, Abirami Nayaki
Mahendra, Little
Sayed, Mohammed E.
Mugri, Maryam H.
Balaji, Thodur Madapusi
Varadarajan, Saranya
Jagannathan, Raghunathan
Srinivasan, Sruthi
Baeshen, Hosam Ali
Mathew, Reji
Patil, Shankargouda
The Expression of Allele Changes in NLRP3 (rs35829419) and IL-1β (+3954) Gene Polymorphisms in Periodontitis and Coronary Artery Disease
title The Expression of Allele Changes in NLRP3 (rs35829419) and IL-1β (+3954) Gene Polymorphisms in Periodontitis and Coronary Artery Disease
title_full The Expression of Allele Changes in NLRP3 (rs35829419) and IL-1β (+3954) Gene Polymorphisms in Periodontitis and Coronary Artery Disease
title_fullStr The Expression of Allele Changes in NLRP3 (rs35829419) and IL-1β (+3954) Gene Polymorphisms in Periodontitis and Coronary Artery Disease
title_full_unstemmed The Expression of Allele Changes in NLRP3 (rs35829419) and IL-1β (+3954) Gene Polymorphisms in Periodontitis and Coronary Artery Disease
title_short The Expression of Allele Changes in NLRP3 (rs35829419) and IL-1β (+3954) Gene Polymorphisms in Periodontitis and Coronary Artery Disease
title_sort expression of allele changes in nlrp3 (rs35829419) and il-1β (+3954) gene polymorphisms in periodontitis and coronary artery disease
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8433916/
https://www.ncbi.nlm.nih.gov/pubmed/34501201
http://dx.doi.org/10.3390/ma14175103
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