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Recent Advances about the Applications of Click Reaction in Chemical Proteomics
Despite significant advances in biological and analytical approaches, a comprehensive portrait of the proteome and its dynamic interactions and modifications remains a challenging goal. Chemical proteomics is a growing area of chemical biology that seeks to design small molecule probes to elucidate...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8434046/ https://www.ncbi.nlm.nih.gov/pubmed/34500797 http://dx.doi.org/10.3390/molecules26175368 |
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author | Yao, Tingting Xu, Xiaowei Huang, Rong |
author_facet | Yao, Tingting Xu, Xiaowei Huang, Rong |
author_sort | Yao, Tingting |
collection | PubMed |
description | Despite significant advances in biological and analytical approaches, a comprehensive portrait of the proteome and its dynamic interactions and modifications remains a challenging goal. Chemical proteomics is a growing area of chemical biology that seeks to design small molecule probes to elucidate protein composition, distribution, and relevant physiological and pharmacological functions. Click chemistry focuses on the development of new combinatorial chemical methods for carbon heteroatom bond (C-X-C) synthesis, which have been utilized extensively in the field of chemical proteomics. Click reactions have various advantages including high yield, harmless by-products, and simple reaction conditions, upon which the molecular diversity can be easily and effectively obtained. This paper reviews the application of click chemistry in proteomics from four aspects: (1) activity-based protein profiling, (2) enzyme-inhibitors screening, (3) protein labeling and modifications, and (4) hybrid monolithic column in proteomic analysis. |
format | Online Article Text |
id | pubmed-8434046 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-84340462021-09-12 Recent Advances about the Applications of Click Reaction in Chemical Proteomics Yao, Tingting Xu, Xiaowei Huang, Rong Molecules Review Despite significant advances in biological and analytical approaches, a comprehensive portrait of the proteome and its dynamic interactions and modifications remains a challenging goal. Chemical proteomics is a growing area of chemical biology that seeks to design small molecule probes to elucidate protein composition, distribution, and relevant physiological and pharmacological functions. Click chemistry focuses on the development of new combinatorial chemical methods for carbon heteroatom bond (C-X-C) synthesis, which have been utilized extensively in the field of chemical proteomics. Click reactions have various advantages including high yield, harmless by-products, and simple reaction conditions, upon which the molecular diversity can be easily and effectively obtained. This paper reviews the application of click chemistry in proteomics from four aspects: (1) activity-based protein profiling, (2) enzyme-inhibitors screening, (3) protein labeling and modifications, and (4) hybrid monolithic column in proteomic analysis. MDPI 2021-09-03 /pmc/articles/PMC8434046/ /pubmed/34500797 http://dx.doi.org/10.3390/molecules26175368 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Yao, Tingting Xu, Xiaowei Huang, Rong Recent Advances about the Applications of Click Reaction in Chemical Proteomics |
title | Recent Advances about the Applications of Click Reaction in Chemical Proteomics |
title_full | Recent Advances about the Applications of Click Reaction in Chemical Proteomics |
title_fullStr | Recent Advances about the Applications of Click Reaction in Chemical Proteomics |
title_full_unstemmed | Recent Advances about the Applications of Click Reaction in Chemical Proteomics |
title_short | Recent Advances about the Applications of Click Reaction in Chemical Proteomics |
title_sort | recent advances about the applications of click reaction in chemical proteomics |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8434046/ https://www.ncbi.nlm.nih.gov/pubmed/34500797 http://dx.doi.org/10.3390/molecules26175368 |
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