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Recent Advances about the Applications of Click Reaction in Chemical Proteomics

Despite significant advances in biological and analytical approaches, a comprehensive portrait of the proteome and its dynamic interactions and modifications remains a challenging goal. Chemical proteomics is a growing area of chemical biology that seeks to design small molecule probes to elucidate...

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Detalles Bibliográficos
Autores principales: Yao, Tingting, Xu, Xiaowei, Huang, Rong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8434046/
https://www.ncbi.nlm.nih.gov/pubmed/34500797
http://dx.doi.org/10.3390/molecules26175368
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author Yao, Tingting
Xu, Xiaowei
Huang, Rong
author_facet Yao, Tingting
Xu, Xiaowei
Huang, Rong
author_sort Yao, Tingting
collection PubMed
description Despite significant advances in biological and analytical approaches, a comprehensive portrait of the proteome and its dynamic interactions and modifications remains a challenging goal. Chemical proteomics is a growing area of chemical biology that seeks to design small molecule probes to elucidate protein composition, distribution, and relevant physiological and pharmacological functions. Click chemistry focuses on the development of new combinatorial chemical methods for carbon heteroatom bond (C-X-C) synthesis, which have been utilized extensively in the field of chemical proteomics. Click reactions have various advantages including high yield, harmless by-products, and simple reaction conditions, upon which the molecular diversity can be easily and effectively obtained. This paper reviews the application of click chemistry in proteomics from four aspects: (1) activity-based protein profiling, (2) enzyme-inhibitors screening, (3) protein labeling and modifications, and (4) hybrid monolithic column in proteomic analysis.
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spelling pubmed-84340462021-09-12 Recent Advances about the Applications of Click Reaction in Chemical Proteomics Yao, Tingting Xu, Xiaowei Huang, Rong Molecules Review Despite significant advances in biological and analytical approaches, a comprehensive portrait of the proteome and its dynamic interactions and modifications remains a challenging goal. Chemical proteomics is a growing area of chemical biology that seeks to design small molecule probes to elucidate protein composition, distribution, and relevant physiological and pharmacological functions. Click chemistry focuses on the development of new combinatorial chemical methods for carbon heteroatom bond (C-X-C) synthesis, which have been utilized extensively in the field of chemical proteomics. Click reactions have various advantages including high yield, harmless by-products, and simple reaction conditions, upon which the molecular diversity can be easily and effectively obtained. This paper reviews the application of click chemistry in proteomics from four aspects: (1) activity-based protein profiling, (2) enzyme-inhibitors screening, (3) protein labeling and modifications, and (4) hybrid monolithic column in proteomic analysis. MDPI 2021-09-03 /pmc/articles/PMC8434046/ /pubmed/34500797 http://dx.doi.org/10.3390/molecules26175368 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Yao, Tingting
Xu, Xiaowei
Huang, Rong
Recent Advances about the Applications of Click Reaction in Chemical Proteomics
title Recent Advances about the Applications of Click Reaction in Chemical Proteomics
title_full Recent Advances about the Applications of Click Reaction in Chemical Proteomics
title_fullStr Recent Advances about the Applications of Click Reaction in Chemical Proteomics
title_full_unstemmed Recent Advances about the Applications of Click Reaction in Chemical Proteomics
title_short Recent Advances about the Applications of Click Reaction in Chemical Proteomics
title_sort recent advances about the applications of click reaction in chemical proteomics
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8434046/
https://www.ncbi.nlm.nih.gov/pubmed/34500797
http://dx.doi.org/10.3390/molecules26175368
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