Sequence Dependent Repair of 1,N(6)-Ethenoadenine by DNA Repair Enzymes ALKBH2, ALKBH3, and AlkB

Mutation patterns of DNA adducts, such as mutational spectra and signatures, are useful tools for diagnostic and prognostic purposes. Mutational spectra of carcinogens derive from three sources: adduct formation, replication bypass, and repair. Here, we consider the repair aspect of 1,N(6)-ethenoade...

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Autores principales: Qi, Rui, Bian, Ke, Chen, Fangyi, Tang, Qi, Zhou, Xianhao, Li, Deyu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Brief Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8434105/
https://www.ncbi.nlm.nih.gov/pubmed/34500720
http://dx.doi.org/10.3390/molecules26175285
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author Qi, Rui
Bian, Ke
Chen, Fangyi
Tang, Qi
Zhou, Xianhao
Li, Deyu
author_facet Qi, Rui
Bian, Ke
Chen, Fangyi
Tang, Qi
Zhou, Xianhao
Li, Deyu
author_sort Qi, Rui
collection PubMed
description Mutation patterns of DNA adducts, such as mutational spectra and signatures, are useful tools for diagnostic and prognostic purposes. Mutational spectra of carcinogens derive from three sources: adduct formation, replication bypass, and repair. Here, we consider the repair aspect of 1,N(6)-ethenoadenine (εA) by the 2-oxoglutarate/Fe(II)-dependent AlkB family enzymes. Specifically, we investigated εA repair across 16 possible sequence contexts (5′/3′ flanking base to εA varied as G/A/T/C). The results revealed that repair efficiency is altered according to sequence, enzyme, and strand context (ss- versus ds-DNA). The methods can be used to study other aspects of mutational spectra or other pathways of repair.
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spelling pubmed-84341052021-09-12 Sequence Dependent Repair of 1,N(6)-Ethenoadenine by DNA Repair Enzymes ALKBH2, ALKBH3, and AlkB Qi, Rui Bian, Ke Chen, Fangyi Tang, Qi Zhou, Xianhao Li, Deyu Molecules Brief Report Mutation patterns of DNA adducts, such as mutational spectra and signatures, are useful tools for diagnostic and prognostic purposes. Mutational spectra of carcinogens derive from three sources: adduct formation, replication bypass, and repair. Here, we consider the repair aspect of 1,N(6)-ethenoadenine (εA) by the 2-oxoglutarate/Fe(II)-dependent AlkB family enzymes. Specifically, we investigated εA repair across 16 possible sequence contexts (5′/3′ flanking base to εA varied as G/A/T/C). The results revealed that repair efficiency is altered according to sequence, enzyme, and strand context (ss- versus ds-DNA). The methods can be used to study other aspects of mutational spectra or other pathways of repair. MDPI 2021-08-31 /pmc/articles/PMC8434105/ /pubmed/34500720 http://dx.doi.org/10.3390/molecules26175285 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Brief Report
Qi, Rui
Bian, Ke
Chen, Fangyi
Tang, Qi
Zhou, Xianhao
Li, Deyu
Sequence Dependent Repair of 1,N(6)-Ethenoadenine by DNA Repair Enzymes ALKBH2, ALKBH3, and AlkB
title Sequence Dependent Repair of 1,N(6)-Ethenoadenine by DNA Repair Enzymes ALKBH2, ALKBH3, and AlkB
title_full Sequence Dependent Repair of 1,N(6)-Ethenoadenine by DNA Repair Enzymes ALKBH2, ALKBH3, and AlkB
title_fullStr Sequence Dependent Repair of 1,N(6)-Ethenoadenine by DNA Repair Enzymes ALKBH2, ALKBH3, and AlkB
title_full_unstemmed Sequence Dependent Repair of 1,N(6)-Ethenoadenine by DNA Repair Enzymes ALKBH2, ALKBH3, and AlkB
title_short Sequence Dependent Repair of 1,N(6)-Ethenoadenine by DNA Repair Enzymes ALKBH2, ALKBH3, and AlkB
title_sort sequence dependent repair of 1,n(6)-ethenoadenine by dna repair enzymes alkbh2, alkbh3, and alkb
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8434105/
https://www.ncbi.nlm.nih.gov/pubmed/34500720
http://dx.doi.org/10.3390/molecules26175285
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