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Delta Sleep-Inducing Peptide Recovers Motor Function in SD Rats after Focal Stroke
Background and Objectives: Mutual effect of the preliminary and therapeutic intranasal treatment of SD rats with DSIP (8 days) on the outcome of focal stroke, induced with intraluminal middle cerebral occlusion (MCAO), was investigated. Materials and Methods: The groups were the following: MCAO + ve...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8434407/ https://www.ncbi.nlm.nih.gov/pubmed/34500605 http://dx.doi.org/10.3390/molecules26175173 |
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author | Tukhovskaya, Elena A. Ismailova, Alina M. Shaykhutdinova, Elvira R. Slashcheva, Gulsara A. Prudchenko, Igor A. Mikhaleva, Inessa I. Khokhlova, Oksana N. Murashev, Arkady N. Ivanov, Vadim T. |
author_facet | Tukhovskaya, Elena A. Ismailova, Alina M. Shaykhutdinova, Elvira R. Slashcheva, Gulsara A. Prudchenko, Igor A. Mikhaleva, Inessa I. Khokhlova, Oksana N. Murashev, Arkady N. Ivanov, Vadim T. |
author_sort | Tukhovskaya, Elena A. |
collection | PubMed |
description | Background and Objectives: Mutual effect of the preliminary and therapeutic intranasal treatment of SD rats with DSIP (8 days) on the outcome of focal stroke, induced with intraluminal middle cerebral occlusion (MCAO), was investigated. Materials and Methods: The groups were the following: MCAO + vehicle, MCAO + DSIP, and SHAM-operated. DSIP or vehicle was applied nasally 60 (±15) minutes prior to the occlusion and for 7 days after reperfusion at dose 120 µg/kg. The battery of behavioral tests was performed on 1, 3, 7, 14, and 21 days after MCAO. Motor coordination and balance and bilateral asymmetry were tested. At the end of the study, animals were euthanized, and their brains were perfused, serial cryoslices were made, and infarction volume in them was calculated. Results: Although brain infarction in DSIP-treated animals was smaller than in vehicle-treated animals, the difference was not significant. However, motor performance in the rotarod test significantly recovered in DSIP-treated animals. Conclusions: Intranasal administration of DSIP in the course of 8 days leads to accelerated recovery of motor functions. |
format | Online Article Text |
id | pubmed-8434407 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-84344072021-09-12 Delta Sleep-Inducing Peptide Recovers Motor Function in SD Rats after Focal Stroke Tukhovskaya, Elena A. Ismailova, Alina M. Shaykhutdinova, Elvira R. Slashcheva, Gulsara A. Prudchenko, Igor A. Mikhaleva, Inessa I. Khokhlova, Oksana N. Murashev, Arkady N. Ivanov, Vadim T. Molecules Article Background and Objectives: Mutual effect of the preliminary and therapeutic intranasal treatment of SD rats with DSIP (8 days) on the outcome of focal stroke, induced with intraluminal middle cerebral occlusion (MCAO), was investigated. Materials and Methods: The groups were the following: MCAO + vehicle, MCAO + DSIP, and SHAM-operated. DSIP or vehicle was applied nasally 60 (±15) minutes prior to the occlusion and for 7 days after reperfusion at dose 120 µg/kg. The battery of behavioral tests was performed on 1, 3, 7, 14, and 21 days after MCAO. Motor coordination and balance and bilateral asymmetry were tested. At the end of the study, animals were euthanized, and their brains were perfused, serial cryoslices were made, and infarction volume in them was calculated. Results: Although brain infarction in DSIP-treated animals was smaller than in vehicle-treated animals, the difference was not significant. However, motor performance in the rotarod test significantly recovered in DSIP-treated animals. Conclusions: Intranasal administration of DSIP in the course of 8 days leads to accelerated recovery of motor functions. MDPI 2021-08-26 /pmc/articles/PMC8434407/ /pubmed/34500605 http://dx.doi.org/10.3390/molecules26175173 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Tukhovskaya, Elena A. Ismailova, Alina M. Shaykhutdinova, Elvira R. Slashcheva, Gulsara A. Prudchenko, Igor A. Mikhaleva, Inessa I. Khokhlova, Oksana N. Murashev, Arkady N. Ivanov, Vadim T. Delta Sleep-Inducing Peptide Recovers Motor Function in SD Rats after Focal Stroke |
title | Delta Sleep-Inducing Peptide Recovers Motor Function in SD Rats after Focal Stroke |
title_full | Delta Sleep-Inducing Peptide Recovers Motor Function in SD Rats after Focal Stroke |
title_fullStr | Delta Sleep-Inducing Peptide Recovers Motor Function in SD Rats after Focal Stroke |
title_full_unstemmed | Delta Sleep-Inducing Peptide Recovers Motor Function in SD Rats after Focal Stroke |
title_short | Delta Sleep-Inducing Peptide Recovers Motor Function in SD Rats after Focal Stroke |
title_sort | delta sleep-inducing peptide recovers motor function in sd rats after focal stroke |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8434407/ https://www.ncbi.nlm.nih.gov/pubmed/34500605 http://dx.doi.org/10.3390/molecules26175173 |
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