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High-volume sampler for size-selective sampling of bioaerosols including viruses

Owing to the recent global spread of the new coronavirus SARS-CoV-2, the development of technology to effectively detect viruses in crowded public places is urgently needed. In this study, a three-stage high-volume bioaerosol sampler was developed for the size-selective sampling of bioaerosols throu...

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Autores principales: Lim, Jun-Hyung, Nam, Sang Hwan, Kim, Jongwoo, Kim, Nam Hoon, Park, Gun-Soo, Maeng, Jin-Soo, Yook, Se-Jin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Ltd. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8435071/
https://www.ncbi.nlm.nih.gov/pubmed/34539212
http://dx.doi.org/10.1016/j.atmosenv.2021.118720
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author Lim, Jun-Hyung
Nam, Sang Hwan
Kim, Jongwoo
Kim, Nam Hoon
Park, Gun-Soo
Maeng, Jin-Soo
Yook, Se-Jin
author_facet Lim, Jun-Hyung
Nam, Sang Hwan
Kim, Jongwoo
Kim, Nam Hoon
Park, Gun-Soo
Maeng, Jin-Soo
Yook, Se-Jin
author_sort Lim, Jun-Hyung
collection PubMed
description Owing to the recent global spread of the new coronavirus SARS-CoV-2, the development of technology to effectively detect viruses in crowded public places is urgently needed. In this study, a three-stage high-volume bioaerosol sampler was developed for the size-selective sampling of bioaerosols through the suction of air at a high flow rate of 1000 L/min. In stage 1, an omnidirectional inlet cyclone separator that can draw air from all directions was applied to collect bioaerosols larger than 10 μm in the collection fluid. In stage 2, an axial flow cyclone separator was used to collect bioaerosols sized between 2.5 and 10 μm in the collection fluid. In stage 3, bioaerosols smaller than 2.5 μm were collected on a filter and extracted in a solution through an elution process using a sodium phosphate buffer. To simulate the suspension of bioparticles including viruses that are attached to other particles in the atmosphere, the aerosol samples were prepared by coagulating aerosolized bacteriophages with Arizona test dust. Then, the coagulated particles were collected for 30 min using the developed bioaerosol sampler, and the samples collected in each stage were analyzed via polymerase chain reaction (PCR) method. The PCR analysis results confirmed that the high-volume bioaerosol sampler enables size-selective bioaerosol sampling even at a high airflow rate of 1000 L/min. The developed high-volume bioaerosol sampler will be useful in detecting viruses through PCR analysis because it can collect bioaerosols within a specific size range.
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spelling pubmed-84350712021-09-13 High-volume sampler for size-selective sampling of bioaerosols including viruses Lim, Jun-Hyung Nam, Sang Hwan Kim, Jongwoo Kim, Nam Hoon Park, Gun-Soo Maeng, Jin-Soo Yook, Se-Jin Atmos Environ (1994) Article Owing to the recent global spread of the new coronavirus SARS-CoV-2, the development of technology to effectively detect viruses in crowded public places is urgently needed. In this study, a three-stage high-volume bioaerosol sampler was developed for the size-selective sampling of bioaerosols through the suction of air at a high flow rate of 1000 L/min. In stage 1, an omnidirectional inlet cyclone separator that can draw air from all directions was applied to collect bioaerosols larger than 10 μm in the collection fluid. In stage 2, an axial flow cyclone separator was used to collect bioaerosols sized between 2.5 and 10 μm in the collection fluid. In stage 3, bioaerosols smaller than 2.5 μm were collected on a filter and extracted in a solution through an elution process using a sodium phosphate buffer. To simulate the suspension of bioparticles including viruses that are attached to other particles in the atmosphere, the aerosol samples were prepared by coagulating aerosolized bacteriophages with Arizona test dust. Then, the coagulated particles were collected for 30 min using the developed bioaerosol sampler, and the samples collected in each stage were analyzed via polymerase chain reaction (PCR) method. The PCR analysis results confirmed that the high-volume bioaerosol sampler enables size-selective bioaerosol sampling even at a high airflow rate of 1000 L/min. The developed high-volume bioaerosol sampler will be useful in detecting viruses through PCR analysis because it can collect bioaerosols within a specific size range. Elsevier Ltd. 2021-11-15 2021-09-12 /pmc/articles/PMC8435071/ /pubmed/34539212 http://dx.doi.org/10.1016/j.atmosenv.2021.118720 Text en © 2021 Elsevier Ltd. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Lim, Jun-Hyung
Nam, Sang Hwan
Kim, Jongwoo
Kim, Nam Hoon
Park, Gun-Soo
Maeng, Jin-Soo
Yook, Se-Jin
High-volume sampler for size-selective sampling of bioaerosols including viruses
title High-volume sampler for size-selective sampling of bioaerosols including viruses
title_full High-volume sampler for size-selective sampling of bioaerosols including viruses
title_fullStr High-volume sampler for size-selective sampling of bioaerosols including viruses
title_full_unstemmed High-volume sampler for size-selective sampling of bioaerosols including viruses
title_short High-volume sampler for size-selective sampling of bioaerosols including viruses
title_sort high-volume sampler for size-selective sampling of bioaerosols including viruses
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8435071/
https://www.ncbi.nlm.nih.gov/pubmed/34539212
http://dx.doi.org/10.1016/j.atmosenv.2021.118720
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