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Synergistic interaction between the type III secretion system of the endophytic bacterium Pantoea agglomerans DAPP‐PG 734 and the virulence of the causal agent of olive knot Pseudomonas savastanoi pv. savastanoi DAPP‐PG 722
The endophytic bacterium Pantoea agglomerans DAPP‐PG 734 was previously isolated from olive knots caused by infection with Pseudomonas savastanoi pv. savastanoi DAPP‐PG 722. Whole‐genome analysis of this P. agglomerans strain revealed the presence of a Hypersensitive response and pathogenicity (Hrp)...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8435235/ https://www.ncbi.nlm.nih.gov/pubmed/34268839 http://dx.doi.org/10.1111/mpp.13105 |
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author | Moretti, Chiaraluce Rezzonico, Fabio Orfei, Benedetta Cortese, Chiara Moreno‐Pérez, Alba van den Burg, Harrold A. Onofri, Andrea Firrao, Giuseppe Ramos, Cayo Smits, Theo H. M. Buonaurio, Roberto |
author_facet | Moretti, Chiaraluce Rezzonico, Fabio Orfei, Benedetta Cortese, Chiara Moreno‐Pérez, Alba van den Burg, Harrold A. Onofri, Andrea Firrao, Giuseppe Ramos, Cayo Smits, Theo H. M. Buonaurio, Roberto |
author_sort | Moretti, Chiaraluce |
collection | PubMed |
description | The endophytic bacterium Pantoea agglomerans DAPP‐PG 734 was previously isolated from olive knots caused by infection with Pseudomonas savastanoi pv. savastanoi DAPP‐PG 722. Whole‐genome analysis of this P. agglomerans strain revealed the presence of a Hypersensitive response and pathogenicity (Hrp) type III secretion system (T3SS). To assess the role of the P. agglomerans T3SS in the interaction with P. savastanoi pv. savastanoi, we generated independent knockout mutants in three Hrp genes of the P. agglomerans DAPP‐PG 734 T3SS (hrpJ, hrpN, and hrpY). In contrast to the wildtype control, all three mutants failed to cause a hypersensitive response when infiltrated in tobacco leaves, suggesting that P. agglomerans T3SS is functional and injects effector proteins in plant cells. In contrast to P. savastanoi pv. savastanoi DAPP‐PG 722, the wildtype strain P. agglomerans DAPP‐PG 734 and its Hrp T3SS mutants did not cause olive knot disease in 1‐year‐old olive plants. Coinoculation of P. savastanoi pv. savastanoi with P. agglomerans wildtype strains did not significantly change the knot size, while the DAPP‐PG 734 hrpY mutant induced a significant decrease in knot size, which could be complemented by providing hrpY on a plasmid. By epifluorescence microscopy and confocal laser scanning microscopy, we found that the localization patterns in knots were nonoverlapping for P. savastanoi pv. savastanoi and P. agglomerans when coinoculated. Our results suggest that suppression of olive plant defences mediated by the Hrp T3SS of P. agglomerans DAPP‐PG 734 positively impacts the virulence of P. savastanoi pv. savastanoi DAPP‐PG 722. |
format | Online Article Text |
id | pubmed-8435235 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-84352352021-09-15 Synergistic interaction between the type III secretion system of the endophytic bacterium Pantoea agglomerans DAPP‐PG 734 and the virulence of the causal agent of olive knot Pseudomonas savastanoi pv. savastanoi DAPP‐PG 722 Moretti, Chiaraluce Rezzonico, Fabio Orfei, Benedetta Cortese, Chiara Moreno‐Pérez, Alba van den Burg, Harrold A. Onofri, Andrea Firrao, Giuseppe Ramos, Cayo Smits, Theo H. M. Buonaurio, Roberto Mol Plant Pathol Original Articles The endophytic bacterium Pantoea agglomerans DAPP‐PG 734 was previously isolated from olive knots caused by infection with Pseudomonas savastanoi pv. savastanoi DAPP‐PG 722. Whole‐genome analysis of this P. agglomerans strain revealed the presence of a Hypersensitive response and pathogenicity (Hrp) type III secretion system (T3SS). To assess the role of the P. agglomerans T3SS in the interaction with P. savastanoi pv. savastanoi, we generated independent knockout mutants in three Hrp genes of the P. agglomerans DAPP‐PG 734 T3SS (hrpJ, hrpN, and hrpY). In contrast to the wildtype control, all three mutants failed to cause a hypersensitive response when infiltrated in tobacco leaves, suggesting that P. agglomerans T3SS is functional and injects effector proteins in plant cells. In contrast to P. savastanoi pv. savastanoi DAPP‐PG 722, the wildtype strain P. agglomerans DAPP‐PG 734 and its Hrp T3SS mutants did not cause olive knot disease in 1‐year‐old olive plants. Coinoculation of P. savastanoi pv. savastanoi with P. agglomerans wildtype strains did not significantly change the knot size, while the DAPP‐PG 734 hrpY mutant induced a significant decrease in knot size, which could be complemented by providing hrpY on a plasmid. By epifluorescence microscopy and confocal laser scanning microscopy, we found that the localization patterns in knots were nonoverlapping for P. savastanoi pv. savastanoi and P. agglomerans when coinoculated. Our results suggest that suppression of olive plant defences mediated by the Hrp T3SS of P. agglomerans DAPP‐PG 734 positively impacts the virulence of P. savastanoi pv. savastanoi DAPP‐PG 722. John Wiley and Sons Inc. 2021-07-16 /pmc/articles/PMC8435235/ /pubmed/34268839 http://dx.doi.org/10.1111/mpp.13105 Text en © 2021 The Authors. Molecular Plant Pathology published by British Society for Plant Pathology and John Wiley & Sons Ltd. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
spellingShingle | Original Articles Moretti, Chiaraluce Rezzonico, Fabio Orfei, Benedetta Cortese, Chiara Moreno‐Pérez, Alba van den Burg, Harrold A. Onofri, Andrea Firrao, Giuseppe Ramos, Cayo Smits, Theo H. M. Buonaurio, Roberto Synergistic interaction between the type III secretion system of the endophytic bacterium Pantoea agglomerans DAPP‐PG 734 and the virulence of the causal agent of olive knot Pseudomonas savastanoi pv. savastanoi DAPP‐PG 722 |
title | Synergistic interaction between the type III secretion system of the endophytic bacterium Pantoea agglomerans DAPP‐PG 734 and the virulence of the causal agent of olive knot Pseudomonas savastanoi pv. savastanoi DAPP‐PG 722 |
title_full | Synergistic interaction between the type III secretion system of the endophytic bacterium Pantoea agglomerans DAPP‐PG 734 and the virulence of the causal agent of olive knot Pseudomonas savastanoi pv. savastanoi DAPP‐PG 722 |
title_fullStr | Synergistic interaction between the type III secretion system of the endophytic bacterium Pantoea agglomerans DAPP‐PG 734 and the virulence of the causal agent of olive knot Pseudomonas savastanoi pv. savastanoi DAPP‐PG 722 |
title_full_unstemmed | Synergistic interaction between the type III secretion system of the endophytic bacterium Pantoea agglomerans DAPP‐PG 734 and the virulence of the causal agent of olive knot Pseudomonas savastanoi pv. savastanoi DAPP‐PG 722 |
title_short | Synergistic interaction between the type III secretion system of the endophytic bacterium Pantoea agglomerans DAPP‐PG 734 and the virulence of the causal agent of olive knot Pseudomonas savastanoi pv. savastanoi DAPP‐PG 722 |
title_sort | synergistic interaction between the type iii secretion system of the endophytic bacterium pantoea agglomerans dapp‐pg 734 and the virulence of the causal agent of olive knot pseudomonas savastanoi pv. savastanoi dapp‐pg 722 |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8435235/ https://www.ncbi.nlm.nih.gov/pubmed/34268839 http://dx.doi.org/10.1111/mpp.13105 |
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