A new flow cytometry assay to measure antibody-dependent cellular cytotoxicity against SARS-CoV-2 Spike-expressing cells

Antibodies can engage specific receptors at the surface of effector cells and mediate several functions beyond viral neutralization. Increasing evidence suggests that Fc-mediated effector functions, such as antibody-dependent cellular cytotoxicity (ADCC), have an important role in protection against...

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Autores principales: Beaudoin-Bussières, Guillaume, Richard, Jonathan, Prévost, Jérémie, Goyette, Guillaume, Finzi, Andrés
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8435374/
https://www.ncbi.nlm.nih.gov/pubmed/34541555
http://dx.doi.org/10.1016/j.xpro.2021.100851
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author Beaudoin-Bussières, Guillaume
Richard, Jonathan
Prévost, Jérémie
Goyette, Guillaume
Finzi, Andrés
author_facet Beaudoin-Bussières, Guillaume
Richard, Jonathan
Prévost, Jérémie
Goyette, Guillaume
Finzi, Andrés
author_sort Beaudoin-Bussières, Guillaume
collection PubMed
description Antibodies can engage specific receptors at the surface of effector cells and mediate several functions beyond viral neutralization. Increasing evidence suggests that Fc-mediated effector functions, such as antibody-dependent cellular cytotoxicity (ADCC), have an important role in protection against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections. We engineered a cell line stably expressing a GFP-tagged SARS-CoV-2 spike to measure ADCC. This protocol provides an optimized way of measuring ADCC activity mediated by anti-SARS-CoV-2 Spike monoclonal antibodies or plasma from previously infected or vaccinated individuals. For complete details on the use and execution of this protocol, please refer to Anand et al. (2021b).
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spelling pubmed-84353742021-09-13 A new flow cytometry assay to measure antibody-dependent cellular cytotoxicity against SARS-CoV-2 Spike-expressing cells Beaudoin-Bussières, Guillaume Richard, Jonathan Prévost, Jérémie Goyette, Guillaume Finzi, Andrés STAR Protoc Protocol Antibodies can engage specific receptors at the surface of effector cells and mediate several functions beyond viral neutralization. Increasing evidence suggests that Fc-mediated effector functions, such as antibody-dependent cellular cytotoxicity (ADCC), have an important role in protection against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections. We engineered a cell line stably expressing a GFP-tagged SARS-CoV-2 spike to measure ADCC. This protocol provides an optimized way of measuring ADCC activity mediated by anti-SARS-CoV-2 Spike monoclonal antibodies or plasma from previously infected or vaccinated individuals. For complete details on the use and execution of this protocol, please refer to Anand et al. (2021b). Elsevier 2021-09-13 /pmc/articles/PMC8435374/ /pubmed/34541555 http://dx.doi.org/10.1016/j.xpro.2021.100851 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Beaudoin-Bussières, Guillaume
Richard, Jonathan
Prévost, Jérémie
Goyette, Guillaume
Finzi, Andrés
A new flow cytometry assay to measure antibody-dependent cellular cytotoxicity against SARS-CoV-2 Spike-expressing cells
title A new flow cytometry assay to measure antibody-dependent cellular cytotoxicity against SARS-CoV-2 Spike-expressing cells
title_full A new flow cytometry assay to measure antibody-dependent cellular cytotoxicity against SARS-CoV-2 Spike-expressing cells
title_fullStr A new flow cytometry assay to measure antibody-dependent cellular cytotoxicity against SARS-CoV-2 Spike-expressing cells
title_full_unstemmed A new flow cytometry assay to measure antibody-dependent cellular cytotoxicity against SARS-CoV-2 Spike-expressing cells
title_short A new flow cytometry assay to measure antibody-dependent cellular cytotoxicity against SARS-CoV-2 Spike-expressing cells
title_sort new flow cytometry assay to measure antibody-dependent cellular cytotoxicity against sars-cov-2 spike-expressing cells
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8435374/
https://www.ncbi.nlm.nih.gov/pubmed/34541555
http://dx.doi.org/10.1016/j.xpro.2021.100851
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