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Functional variation (Q63R) in the cannabinoid CB2 receptor may affect the severity of COVID-19: a human study and molecular docking
Evidence supports a role of host genetic diversity in the clinical course of coronavirus disease 2019 (COVID-19). Variation in the cannabinoid CB2 receptor gene (CNR2) could affect the regulatory action of endocannabinoids on the immune system, resulting in an increased risk of various inflammatory...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Vienna
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8435402/ https://www.ncbi.nlm.nih.gov/pubmed/34514519 http://dx.doi.org/10.1007/s00705-021-05223-7 |
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author | Rastegar, Mostafa Samadizadeh, Saeed Yasaghi, Mohammad Moradi, Abdolvahab Tabarraei, Alijan Salimi, Vahid Tahamtan, Alireza |
author_facet | Rastegar, Mostafa Samadizadeh, Saeed Yasaghi, Mohammad Moradi, Abdolvahab Tabarraei, Alijan Salimi, Vahid Tahamtan, Alireza |
author_sort | Rastegar, Mostafa |
collection | PubMed |
description | Evidence supports a role of host genetic diversity in the clinical course of coronavirus disease 2019 (COVID-19). Variation in the cannabinoid CB2 receptor gene (CNR2) could affect the regulatory action of endocannabinoids on the immune system, resulting in an increased risk of various inflammatory diseases. The present study investigated the relationship between the CNR2-Q63R variant and COVID-19 severity. A total of 200 Iranian COVID-19 patients were enrolled in the study and genotyped using a TaqMan assay. The co-dominant, dominant, recessive, over-dominant, and additive inheritance models were analyzed using SNPStats software. In silico molecular docking was also performed to simulate the effects of the Q63R variation on CB2 binding with a ligand and with the G-protein. A significant difference in the Q63R allele and genotype distribution was found between expired and discharged COVID-19 patients in co-dominant, recessive, and additive inheritance models. The molecular docking results showed that the predicted structure of mutant CB2 (63R type) could not bind to the G-protein in the correct position. The data indicated that the Q63R variation in the CNR2 gene may affect the severity of COVID-19. Identification of genes related to susceptibility and severity of COVID-19 may lead to specific targets for drug repurposing or development. GRAPHIC ABSTRACT: [Image: see text] |
format | Online Article Text |
id | pubmed-8435402 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Springer Vienna |
record_format | MEDLINE/PubMed |
spelling | pubmed-84354022021-09-13 Functional variation (Q63R) in the cannabinoid CB2 receptor may affect the severity of COVID-19: a human study and molecular docking Rastegar, Mostafa Samadizadeh, Saeed Yasaghi, Mohammad Moradi, Abdolvahab Tabarraei, Alijan Salimi, Vahid Tahamtan, Alireza Arch Virol Original Article Evidence supports a role of host genetic diversity in the clinical course of coronavirus disease 2019 (COVID-19). Variation in the cannabinoid CB2 receptor gene (CNR2) could affect the regulatory action of endocannabinoids on the immune system, resulting in an increased risk of various inflammatory diseases. The present study investigated the relationship between the CNR2-Q63R variant and COVID-19 severity. A total of 200 Iranian COVID-19 patients were enrolled in the study and genotyped using a TaqMan assay. The co-dominant, dominant, recessive, over-dominant, and additive inheritance models were analyzed using SNPStats software. In silico molecular docking was also performed to simulate the effects of the Q63R variation on CB2 binding with a ligand and with the G-protein. A significant difference in the Q63R allele and genotype distribution was found between expired and discharged COVID-19 patients in co-dominant, recessive, and additive inheritance models. The molecular docking results showed that the predicted structure of mutant CB2 (63R type) could not bind to the G-protein in the correct position. The data indicated that the Q63R variation in the CNR2 gene may affect the severity of COVID-19. Identification of genes related to susceptibility and severity of COVID-19 may lead to specific targets for drug repurposing or development. GRAPHIC ABSTRACT: [Image: see text] Springer Vienna 2021-09-13 2021 /pmc/articles/PMC8435402/ /pubmed/34514519 http://dx.doi.org/10.1007/s00705-021-05223-7 Text en © The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature 2021 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic. |
spellingShingle | Original Article Rastegar, Mostafa Samadizadeh, Saeed Yasaghi, Mohammad Moradi, Abdolvahab Tabarraei, Alijan Salimi, Vahid Tahamtan, Alireza Functional variation (Q63R) in the cannabinoid CB2 receptor may affect the severity of COVID-19: a human study and molecular docking |
title | Functional variation (Q63R) in the cannabinoid CB2 receptor may affect the severity of COVID-19: a human study and molecular docking |
title_full | Functional variation (Q63R) in the cannabinoid CB2 receptor may affect the severity of COVID-19: a human study and molecular docking |
title_fullStr | Functional variation (Q63R) in the cannabinoid CB2 receptor may affect the severity of COVID-19: a human study and molecular docking |
title_full_unstemmed | Functional variation (Q63R) in the cannabinoid CB2 receptor may affect the severity of COVID-19: a human study and molecular docking |
title_short | Functional variation (Q63R) in the cannabinoid CB2 receptor may affect the severity of COVID-19: a human study and molecular docking |
title_sort | functional variation (q63r) in the cannabinoid cb2 receptor may affect the severity of covid-19: a human study and molecular docking |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8435402/ https://www.ncbi.nlm.nih.gov/pubmed/34514519 http://dx.doi.org/10.1007/s00705-021-05223-7 |
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