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Fluid shear stress regulates osteoblast proliferation and apoptosis via the lncRNA TUG1/miR‐34a/FGFR1 axis

LncRNAs and microRNAs play critical roles in osteoblast differentiation and bone formation. However, their exact roles in osteoblasts under fluid shear stress (FSS) and the possible mechanisms remain unclear. The aim of this study was to explore whether and how miR‐34a regulates osteoblast prolifera...

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Autores principales: Wang, Xingwen, He, Jinwen, Wang, Hong, Zhao, Dacheng, Geng, Bin, Wang, Shenghong, An, Jiangdong, Wang, Cuifang, Han, Hua, Xia, Yayi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8435422/
https://www.ncbi.nlm.nih.gov/pubmed/34350720
http://dx.doi.org/10.1111/jcmm.16829
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author Wang, Xingwen
He, Jinwen
Wang, Hong
Zhao, Dacheng
Geng, Bin
Wang, Shenghong
An, Jiangdong
Wang, Cuifang
Han, Hua
Xia, Yayi
author_facet Wang, Xingwen
He, Jinwen
Wang, Hong
Zhao, Dacheng
Geng, Bin
Wang, Shenghong
An, Jiangdong
Wang, Cuifang
Han, Hua
Xia, Yayi
author_sort Wang, Xingwen
collection PubMed
description LncRNAs and microRNAs play critical roles in osteoblast differentiation and bone formation. However, their exact roles in osteoblasts under fluid shear stress (FSS) and the possible mechanisms remain unclear. The aim of this study was to explore whether and how miR‐34a regulates osteoblast proliferation and apoptosis under FSS. In this study, FSS down‐regulated miR‐34a levels of MC3T3‐E1 cells. MiR‐34a up‐regulation attenuated FSS‐induced promotion of proliferation and suppression of apoptosis. Luciferase reporter assay revealed that miR‐34a directly targeted FGFR1. Moreover, miR‐34a regulated osteoblast proliferation and apoptosis via FGFR1. Further, we validated that lncRNA TUG1 acted as a competing endogenous RNA (ceRNA) to interact with miR‐34a and up‐regulate FGFR1 protein expression. Furthermore, lncRNA TUG1 could promote proliferation and inhibit apoptosis. Taken together, our study revealed the key role of the lncRNA TUG1/miR‐34a/FGFR1 axis in FSS‐regulated osteoblast proliferation and apoptosis and may provide potential therapeutic targets for osteoporosis.
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spelling pubmed-84354222021-09-15 Fluid shear stress regulates osteoblast proliferation and apoptosis via the lncRNA TUG1/miR‐34a/FGFR1 axis Wang, Xingwen He, Jinwen Wang, Hong Zhao, Dacheng Geng, Bin Wang, Shenghong An, Jiangdong Wang, Cuifang Han, Hua Xia, Yayi J Cell Mol Med Original Articles LncRNAs and microRNAs play critical roles in osteoblast differentiation and bone formation. However, their exact roles in osteoblasts under fluid shear stress (FSS) and the possible mechanisms remain unclear. The aim of this study was to explore whether and how miR‐34a regulates osteoblast proliferation and apoptosis under FSS. In this study, FSS down‐regulated miR‐34a levels of MC3T3‐E1 cells. MiR‐34a up‐regulation attenuated FSS‐induced promotion of proliferation and suppression of apoptosis. Luciferase reporter assay revealed that miR‐34a directly targeted FGFR1. Moreover, miR‐34a regulated osteoblast proliferation and apoptosis via FGFR1. Further, we validated that lncRNA TUG1 acted as a competing endogenous RNA (ceRNA) to interact with miR‐34a and up‐regulate FGFR1 protein expression. Furthermore, lncRNA TUG1 could promote proliferation and inhibit apoptosis. Taken together, our study revealed the key role of the lncRNA TUG1/miR‐34a/FGFR1 axis in FSS‐regulated osteoblast proliferation and apoptosis and may provide potential therapeutic targets for osteoporosis. John Wiley and Sons Inc. 2021-08-05 2021-09 /pmc/articles/PMC8435422/ /pubmed/34350720 http://dx.doi.org/10.1111/jcmm.16829 Text en © 2021 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Wang, Xingwen
He, Jinwen
Wang, Hong
Zhao, Dacheng
Geng, Bin
Wang, Shenghong
An, Jiangdong
Wang, Cuifang
Han, Hua
Xia, Yayi
Fluid shear stress regulates osteoblast proliferation and apoptosis via the lncRNA TUG1/miR‐34a/FGFR1 axis
title Fluid shear stress regulates osteoblast proliferation and apoptosis via the lncRNA TUG1/miR‐34a/FGFR1 axis
title_full Fluid shear stress regulates osteoblast proliferation and apoptosis via the lncRNA TUG1/miR‐34a/FGFR1 axis
title_fullStr Fluid shear stress regulates osteoblast proliferation and apoptosis via the lncRNA TUG1/miR‐34a/FGFR1 axis
title_full_unstemmed Fluid shear stress regulates osteoblast proliferation and apoptosis via the lncRNA TUG1/miR‐34a/FGFR1 axis
title_short Fluid shear stress regulates osteoblast proliferation and apoptosis via the lncRNA TUG1/miR‐34a/FGFR1 axis
title_sort fluid shear stress regulates osteoblast proliferation and apoptosis via the lncrna tug1/mir‐34a/fgfr1 axis
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8435422/
https://www.ncbi.nlm.nih.gov/pubmed/34350720
http://dx.doi.org/10.1111/jcmm.16829
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