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The Spatial and Temporal Characterization of Gut Microbiota in Broilers

The gut microbiota of chickens plays an important role in host physiology. However, the colonization and prevalence of gut microbiota have not been well-characterized. Here, we performed 16S rRNA gene sequencing on the duodenal, cecal and fecal microbiota of broilers at 1, 7, 21, and 35 days of age...

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Autores principales: Zhou, Qianqian, Lan, Fangren, Li, Xiaochang, Yan, Wei, Sun, Congjiao, Li, Junying, Yang, Ning, Wen, Chaoliang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8435590/
https://www.ncbi.nlm.nih.gov/pubmed/34527716
http://dx.doi.org/10.3389/fvets.2021.712226
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author Zhou, Qianqian
Lan, Fangren
Li, Xiaochang
Yan, Wei
Sun, Congjiao
Li, Junying
Yang, Ning
Wen, Chaoliang
author_facet Zhou, Qianqian
Lan, Fangren
Li, Xiaochang
Yan, Wei
Sun, Congjiao
Li, Junying
Yang, Ning
Wen, Chaoliang
author_sort Zhou, Qianqian
collection PubMed
description The gut microbiota of chickens plays an important role in host physiology. However, the colonization and prevalence of gut microbiota have not been well-characterized. Here, we performed 16S rRNA gene sequencing on the duodenal, cecal and fecal microbiota of broilers at 1, 7, 21, and 35 days of age and characterized the dynamic succession of microbiota across the intestinal tract. Our results showed that Firmicutes was the most abundant phylum detected in each gut site at various ages, while the microbial diversity and composition varied among the duodenum, cecum, and feces at different ages. The microbial diversity and complexity of the cecal microbiota increased with age, gradually achieving stability at 21 days of age. As a specific genus in the cecum, Clostridium_sensu_stricto_1 accounted for 83.50% of the total abundance at 1 day of age, but its relative abundance diminished with age. Regarding the feces, the highest alpha diversity was observed at 1 day of age, significantly separated from the alpha diversity of other ages. In addition, no significant differences were observed in the alpha diversity of duodenal samples among 7, 21, and 35 days of age. The predominant bacterium, Lactobacillus, was relatively low (0.68–6.04%) in the intestinal tract of 1-day-old chicks, whereas its abundance increased substantially at 7 days of age and was higher in the duodenum and feces. Escherichia-Shigella, another predominant bacterium in the chicken intestinal tract, was also found to be highly abundant in fecal samples, and the age-associated dynamic trend coincided with that of Lactobacillus. In addition, several genera, including Blautia, Ruminiclostridium_5, Ruminococcaceae_UCG-014, and [Ruminococcus]_torques_group, which are related to the production of short-chain fatty acids, were identified as biomarker bacteria of the cecum after 21 days of age. These findings shed direct light on the temporal and spatial dynamics of intestinal microbiota and provide new opportunities for the improvement of poultry health and production.
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spelling pubmed-84355902021-09-14 The Spatial and Temporal Characterization of Gut Microbiota in Broilers Zhou, Qianqian Lan, Fangren Li, Xiaochang Yan, Wei Sun, Congjiao Li, Junying Yang, Ning Wen, Chaoliang Front Vet Sci Veterinary Science The gut microbiota of chickens plays an important role in host physiology. However, the colonization and prevalence of gut microbiota have not been well-characterized. Here, we performed 16S rRNA gene sequencing on the duodenal, cecal and fecal microbiota of broilers at 1, 7, 21, and 35 days of age and characterized the dynamic succession of microbiota across the intestinal tract. Our results showed that Firmicutes was the most abundant phylum detected in each gut site at various ages, while the microbial diversity and composition varied among the duodenum, cecum, and feces at different ages. The microbial diversity and complexity of the cecal microbiota increased with age, gradually achieving stability at 21 days of age. As a specific genus in the cecum, Clostridium_sensu_stricto_1 accounted for 83.50% of the total abundance at 1 day of age, but its relative abundance diminished with age. Regarding the feces, the highest alpha diversity was observed at 1 day of age, significantly separated from the alpha diversity of other ages. In addition, no significant differences were observed in the alpha diversity of duodenal samples among 7, 21, and 35 days of age. The predominant bacterium, Lactobacillus, was relatively low (0.68–6.04%) in the intestinal tract of 1-day-old chicks, whereas its abundance increased substantially at 7 days of age and was higher in the duodenum and feces. Escherichia-Shigella, another predominant bacterium in the chicken intestinal tract, was also found to be highly abundant in fecal samples, and the age-associated dynamic trend coincided with that of Lactobacillus. In addition, several genera, including Blautia, Ruminiclostridium_5, Ruminococcaceae_UCG-014, and [Ruminococcus]_torques_group, which are related to the production of short-chain fatty acids, were identified as biomarker bacteria of the cecum after 21 days of age. These findings shed direct light on the temporal and spatial dynamics of intestinal microbiota and provide new opportunities for the improvement of poultry health and production. Frontiers Media S.A. 2021-08-30 /pmc/articles/PMC8435590/ /pubmed/34527716 http://dx.doi.org/10.3389/fvets.2021.712226 Text en Copyright © 2021 Zhou, Lan, Li, Yan, Sun, Li, Yang and Wen. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Zhou, Qianqian
Lan, Fangren
Li, Xiaochang
Yan, Wei
Sun, Congjiao
Li, Junying
Yang, Ning
Wen, Chaoliang
The Spatial and Temporal Characterization of Gut Microbiota in Broilers
title The Spatial and Temporal Characterization of Gut Microbiota in Broilers
title_full The Spatial and Temporal Characterization of Gut Microbiota in Broilers
title_fullStr The Spatial and Temporal Characterization of Gut Microbiota in Broilers
title_full_unstemmed The Spatial and Temporal Characterization of Gut Microbiota in Broilers
title_short The Spatial and Temporal Characterization of Gut Microbiota in Broilers
title_sort spatial and temporal characterization of gut microbiota in broilers
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8435590/
https://www.ncbi.nlm.nih.gov/pubmed/34527716
http://dx.doi.org/10.3389/fvets.2021.712226
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