Cargando…
Microbulb and plantlet formation of a native bulbous flower, Lilium monodelphum M. Bieb, var. Armenum, through tissue culture propagation
The plant Lilium monadelphum M. Bieb. var. armenum (Mıscz. Ex Grossh) Davis et Henderson recognized as the caucasian lily grows in the Eastern Black Sea Region and is a perennial bulbous species belonging to the Liliaceae family. Its flowers, bulbs, and volatile oils are used in the perfume industry...
Autores principales: | , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8435690/ https://www.ncbi.nlm.nih.gov/pubmed/34540598 http://dx.doi.org/10.1016/j.btre.2021.e00665 |
_version_ | 1783751849574137856 |
---|---|
author | AZERİ, F.N. Öztürk, G. |
author_facet | AZERİ, F.N. Öztürk, G. |
author_sort | AZERİ, F.N. |
collection | PubMed |
description | The plant Lilium monadelphum M. Bieb. var. armenum (Mıscz. Ex Grossh) Davis et Henderson recognized as the caucasian lily grows in the Eastern Black Sea Region and is a perennial bulbous species belonging to the Liliaceae family. Its flowers, bulbs, and volatile oils are used in the perfume industry, as a folk medicine to treat eczema and abscess therapy, and as a tranquilizer among regional people respectively. Hence, it is commonly collected from the wild and could become endangered. In this study, we explored the optimal hormone treatment for efficient and rapid induction and production of this species in tissue culture. Explants taken from bulbs were cultured in Murashige and Skoog medium (MS medium, 1962) and modified with TDZ (thidiazuran) (0.5; 1.0; 2.0; 3.0; 5.0 mg/L), after which adventitious buds used for explants during the second stage were subcultured 4 different media supplemented with different concentrations of PAC (paclobutrazol) (0.1; 0.5; 1.0; 2.0 mg/L) to induce microbulbs formation. Microbulbs were cultured in a rooting medium containing IBA (0.5;1.0 mg/L). The highest bud regeneration was observed in medium containing 3.0 mg/L TDZ, 0.25 mg/L NAA and 0.1 mg/L GA3; the highest microbulb formation was observed in medium containing 2.0 mg/L PAC, 0.2 mg/L NAA, and 0.1 mg/L GA3. Besides, plant development from the microbulb was successful for roots in a medium containing 0.5 mg/I IBA and all of the plantlets obtained in tissue culture survived when transferred to the soil. |
format | Online Article Text |
id | pubmed-8435690 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-84356902021-09-17 Microbulb and plantlet formation of a native bulbous flower, Lilium monodelphum M. Bieb, var. Armenum, through tissue culture propagation AZERİ, F.N. Öztürk, G. Biotechnol Rep (Amst) Articles from the Special Issue on Endophytes in biotechnology – the toolbox bridging plant and microbial metabolism; Edited by Gashaw Mamo The plant Lilium monadelphum M. Bieb. var. armenum (Mıscz. Ex Grossh) Davis et Henderson recognized as the caucasian lily grows in the Eastern Black Sea Region and is a perennial bulbous species belonging to the Liliaceae family. Its flowers, bulbs, and volatile oils are used in the perfume industry, as a folk medicine to treat eczema and abscess therapy, and as a tranquilizer among regional people respectively. Hence, it is commonly collected from the wild and could become endangered. In this study, we explored the optimal hormone treatment for efficient and rapid induction and production of this species in tissue culture. Explants taken from bulbs were cultured in Murashige and Skoog medium (MS medium, 1962) and modified with TDZ (thidiazuran) (0.5; 1.0; 2.0; 3.0; 5.0 mg/L), after which adventitious buds used for explants during the second stage were subcultured 4 different media supplemented with different concentrations of PAC (paclobutrazol) (0.1; 0.5; 1.0; 2.0 mg/L) to induce microbulbs formation. Microbulbs were cultured in a rooting medium containing IBA (0.5;1.0 mg/L). The highest bud regeneration was observed in medium containing 3.0 mg/L TDZ, 0.25 mg/L NAA and 0.1 mg/L GA3; the highest microbulb formation was observed in medium containing 2.0 mg/L PAC, 0.2 mg/L NAA, and 0.1 mg/L GA3. Besides, plant development from the microbulb was successful for roots in a medium containing 0.5 mg/I IBA and all of the plantlets obtained in tissue culture survived when transferred to the soil. Elsevier 2021-08-08 /pmc/articles/PMC8435690/ /pubmed/34540598 http://dx.doi.org/10.1016/j.btre.2021.e00665 Text en © 2021 Published by Elsevier B.V. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Articles from the Special Issue on Endophytes in biotechnology – the toolbox bridging plant and microbial metabolism; Edited by Gashaw Mamo AZERİ, F.N. Öztürk, G. Microbulb and plantlet formation of a native bulbous flower, Lilium monodelphum M. Bieb, var. Armenum, through tissue culture propagation |
title | Microbulb and plantlet formation of a native bulbous flower, Lilium monodelphum M. Bieb, var. Armenum, through tissue culture propagation |
title_full | Microbulb and plantlet formation of a native bulbous flower, Lilium monodelphum M. Bieb, var. Armenum, through tissue culture propagation |
title_fullStr | Microbulb and plantlet formation of a native bulbous flower, Lilium monodelphum M. Bieb, var. Armenum, through tissue culture propagation |
title_full_unstemmed | Microbulb and plantlet formation of a native bulbous flower, Lilium monodelphum M. Bieb, var. Armenum, through tissue culture propagation |
title_short | Microbulb and plantlet formation of a native bulbous flower, Lilium monodelphum M. Bieb, var. Armenum, through tissue culture propagation |
title_sort | microbulb and plantlet formation of a native bulbous flower, lilium monodelphum m. bieb, var. armenum, through tissue culture propagation |
topic | Articles from the Special Issue on Endophytes in biotechnology – the toolbox bridging plant and microbial metabolism; Edited by Gashaw Mamo |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8435690/ https://www.ncbi.nlm.nih.gov/pubmed/34540598 http://dx.doi.org/10.1016/j.btre.2021.e00665 |
work_keys_str_mv | AT azerifn microbulbandplantletformationofanativebulbousflowerliliummonodelphummbiebvararmenumthroughtissueculturepropagation AT ozturkg microbulbandplantletformationofanativebulbousflowerliliummonodelphummbiebvararmenumthroughtissueculturepropagation |