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Metagenomic Survey Reveals More Diverse and Abundant Antibiotic Resistance Genes in Municipal Wastewater Than Hospital Wastewater

Alongside antibiotic resistance, co-selection of antibiotics, biocides, and metal resistance is a growing concern. While hospital wastewater is considered a hotspot for antibiotic-resistant bacteria (ARB) and genes (ARGs), the scenario in India, one of the biggest consumers of antibiotics, remains p...

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Autores principales: Zhang, Dengwei, Peng, Ye, Chan, Chak-Lun, On, Hilda, Wai, Hogan Kok-Fung, Shekhawat, Sandeep Singh, Gupta, Akhilendra Bhushan, Varshney, Alok Kumar, Chuanchuen, Rungtip, Zhou, Xudong, Xia, Yankai, Liang, Suisha, Fukuda, Keiji, Medicherla, Krishna Mohan, Tun, Hein M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8435860/
https://www.ncbi.nlm.nih.gov/pubmed/34526976
http://dx.doi.org/10.3389/fmicb.2021.712843
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author Zhang, Dengwei
Peng, Ye
Chan, Chak-Lun
On, Hilda
Wai, Hogan Kok-Fung
Shekhawat, Sandeep Singh
Gupta, Akhilendra Bhushan
Varshney, Alok Kumar
Chuanchuen, Rungtip
Zhou, Xudong
Xia, Yankai
Liang, Suisha
Fukuda, Keiji
Medicherla, Krishna Mohan
Tun, Hein M.
author_facet Zhang, Dengwei
Peng, Ye
Chan, Chak-Lun
On, Hilda
Wai, Hogan Kok-Fung
Shekhawat, Sandeep Singh
Gupta, Akhilendra Bhushan
Varshney, Alok Kumar
Chuanchuen, Rungtip
Zhou, Xudong
Xia, Yankai
Liang, Suisha
Fukuda, Keiji
Medicherla, Krishna Mohan
Tun, Hein M.
author_sort Zhang, Dengwei
collection PubMed
description Alongside antibiotic resistance, co-selection of antibiotics, biocides, and metal resistance is a growing concern. While hospital wastewater is considered a hotspot for antibiotic-resistant bacteria (ARB) and genes (ARGs), the scenario in India, one of the biggest consumers of antibiotics, remains poorly described. In this study, we used metagenomic sequencing to characterize ARGs and biocide/metal resistance genes (BMRGs) in four wastewater treatment plants (WWTPs) in Jaipur City of India. We observed a significantly lower richness and abundance of ARGs in the influent of a WWTP exclusively receiving hospital wastewater when compared to other three WWTPs involving municipal wastewater treatment. Several tetracycline and macrolide-lincosamide-streptogramin resistance genes were enriched in influents of these three municipal wastewater-related treatment plants, whereas hospital wastewater had a higher abundance of genes conferring resistance to disinfectant-related compounds such as synergize and wex-cide-128, reflecting the patterns of antibiotic/disinfectant use. Of note, in the wastewater system with more chemicals, there was a strong correlation between the numbers of ARGs and BMRGs potentially harbored by common hosts. Our study highlights significant influxes of ARGs from non-hospital sources in Jaipur City, and thus more attention should be paid on the emergence of ARGs in general communities.
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spelling pubmed-84358602021-09-14 Metagenomic Survey Reveals More Diverse and Abundant Antibiotic Resistance Genes in Municipal Wastewater Than Hospital Wastewater Zhang, Dengwei Peng, Ye Chan, Chak-Lun On, Hilda Wai, Hogan Kok-Fung Shekhawat, Sandeep Singh Gupta, Akhilendra Bhushan Varshney, Alok Kumar Chuanchuen, Rungtip Zhou, Xudong Xia, Yankai Liang, Suisha Fukuda, Keiji Medicherla, Krishna Mohan Tun, Hein M. Front Microbiol Microbiology Alongside antibiotic resistance, co-selection of antibiotics, biocides, and metal resistance is a growing concern. While hospital wastewater is considered a hotspot for antibiotic-resistant bacteria (ARB) and genes (ARGs), the scenario in India, one of the biggest consumers of antibiotics, remains poorly described. In this study, we used metagenomic sequencing to characterize ARGs and biocide/metal resistance genes (BMRGs) in four wastewater treatment plants (WWTPs) in Jaipur City of India. We observed a significantly lower richness and abundance of ARGs in the influent of a WWTP exclusively receiving hospital wastewater when compared to other three WWTPs involving municipal wastewater treatment. Several tetracycline and macrolide-lincosamide-streptogramin resistance genes were enriched in influents of these three municipal wastewater-related treatment plants, whereas hospital wastewater had a higher abundance of genes conferring resistance to disinfectant-related compounds such as synergize and wex-cide-128, reflecting the patterns of antibiotic/disinfectant use. Of note, in the wastewater system with more chemicals, there was a strong correlation between the numbers of ARGs and BMRGs potentially harbored by common hosts. Our study highlights significant influxes of ARGs from non-hospital sources in Jaipur City, and thus more attention should be paid on the emergence of ARGs in general communities. Frontiers Media S.A. 2021-08-30 /pmc/articles/PMC8435860/ /pubmed/34526976 http://dx.doi.org/10.3389/fmicb.2021.712843 Text en Copyright © 2021 Zhang, Peng, Chan, On, Wai, Shekhawat, Gupta, Varshney, Chuanchuen, Zhou, Xia, Liang, Fukuda, Medicherla and Tun. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Zhang, Dengwei
Peng, Ye
Chan, Chak-Lun
On, Hilda
Wai, Hogan Kok-Fung
Shekhawat, Sandeep Singh
Gupta, Akhilendra Bhushan
Varshney, Alok Kumar
Chuanchuen, Rungtip
Zhou, Xudong
Xia, Yankai
Liang, Suisha
Fukuda, Keiji
Medicherla, Krishna Mohan
Tun, Hein M.
Metagenomic Survey Reveals More Diverse and Abundant Antibiotic Resistance Genes in Municipal Wastewater Than Hospital Wastewater
title Metagenomic Survey Reveals More Diverse and Abundant Antibiotic Resistance Genes in Municipal Wastewater Than Hospital Wastewater
title_full Metagenomic Survey Reveals More Diverse and Abundant Antibiotic Resistance Genes in Municipal Wastewater Than Hospital Wastewater
title_fullStr Metagenomic Survey Reveals More Diverse and Abundant Antibiotic Resistance Genes in Municipal Wastewater Than Hospital Wastewater
title_full_unstemmed Metagenomic Survey Reveals More Diverse and Abundant Antibiotic Resistance Genes in Municipal Wastewater Than Hospital Wastewater
title_short Metagenomic Survey Reveals More Diverse and Abundant Antibiotic Resistance Genes in Municipal Wastewater Than Hospital Wastewater
title_sort metagenomic survey reveals more diverse and abundant antibiotic resistance genes in municipal wastewater than hospital wastewater
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8435860/
https://www.ncbi.nlm.nih.gov/pubmed/34526976
http://dx.doi.org/10.3389/fmicb.2021.712843
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