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Anti-allodynic and promotive effect on inhibitory synaptic transmission of riluzole in rat spinal dorsal horn
Riluzole (2-amino-6-(trifluoromethoxy)benzothiazole) is a drug known for its inhibitory effect on glutamatergic transmission and its anti-nociceptive and anti-allodynic effects in neuropathic pain rat models. Riluzole also has an enhancing effect on GABAergic synaptic transmission. However, the effe...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8435917/ https://www.ncbi.nlm.nih.gov/pubmed/34541342 http://dx.doi.org/10.1016/j.bbrep.2021.101130 |
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author | Taiji, Ryo Yamanaka, Manabu Taniguchi, Wataru Nishio, Naoko Tsutsui, Shunji Nakatsuka, Terumasa Yamada, Hiroshi |
author_facet | Taiji, Ryo Yamanaka, Manabu Taniguchi, Wataru Nishio, Naoko Tsutsui, Shunji Nakatsuka, Terumasa Yamada, Hiroshi |
author_sort | Taiji, Ryo |
collection | PubMed |
description | Riluzole (2-amino-6-(trifluoromethoxy)benzothiazole) is a drug known for its inhibitory effect on glutamatergic transmission and its anti-nociceptive and anti-allodynic effects in neuropathic pain rat models. Riluzole also has an enhancing effect on GABAergic synaptic transmission. However, the effect on the spinal dorsal horn, which plays an important role in modulating nociceptive transmission, remains unknown. We investigated the ameliorating effect of riluzole on mechanical allodynia using the von Frey test in a rat model of neuropathic pain and analyzed the synaptic action of riluzole on inhibitory synaptic transmission in substantia gelatinosa (SG) neurons using whole-cell patch clamp recordings. We found that single-dose intraperitoneal riluzole (4 mg/kg) administration effectively attenuated mechanical allodynia in the short term in a rat model of neuropathic pain. Moreover, 300 μM riluzole induced an outward current in rat SG neurons. The outward current induced by riluzole was not suppressed in the presence of tetrodotoxin. Furthermore, we found that the outward current was suppressed by simultaneous bicuculline and strychnine application, but not by strychnine alone. Altogether, these results suggest that riluzole enhances inhibitory synaptic transmission monosynaptically by potentiating GABAergic synaptic transmission in the rat spinal dorsal horn. |
format | Online Article Text |
id | pubmed-8435917 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-84359172021-09-17 Anti-allodynic and promotive effect on inhibitory synaptic transmission of riluzole in rat spinal dorsal horn Taiji, Ryo Yamanaka, Manabu Taniguchi, Wataru Nishio, Naoko Tsutsui, Shunji Nakatsuka, Terumasa Yamada, Hiroshi Biochem Biophys Rep Research Article Riluzole (2-amino-6-(trifluoromethoxy)benzothiazole) is a drug known for its inhibitory effect on glutamatergic transmission and its anti-nociceptive and anti-allodynic effects in neuropathic pain rat models. Riluzole also has an enhancing effect on GABAergic synaptic transmission. However, the effect on the spinal dorsal horn, which plays an important role in modulating nociceptive transmission, remains unknown. We investigated the ameliorating effect of riluzole on mechanical allodynia using the von Frey test in a rat model of neuropathic pain and analyzed the synaptic action of riluzole on inhibitory synaptic transmission in substantia gelatinosa (SG) neurons using whole-cell patch clamp recordings. We found that single-dose intraperitoneal riluzole (4 mg/kg) administration effectively attenuated mechanical allodynia in the short term in a rat model of neuropathic pain. Moreover, 300 μM riluzole induced an outward current in rat SG neurons. The outward current induced by riluzole was not suppressed in the presence of tetrodotoxin. Furthermore, we found that the outward current was suppressed by simultaneous bicuculline and strychnine application, but not by strychnine alone. Altogether, these results suggest that riluzole enhances inhibitory synaptic transmission monosynaptically by potentiating GABAergic synaptic transmission in the rat spinal dorsal horn. Elsevier 2021-09-10 /pmc/articles/PMC8435917/ /pubmed/34541342 http://dx.doi.org/10.1016/j.bbrep.2021.101130 Text en © 2021 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Research Article Taiji, Ryo Yamanaka, Manabu Taniguchi, Wataru Nishio, Naoko Tsutsui, Shunji Nakatsuka, Terumasa Yamada, Hiroshi Anti-allodynic and promotive effect on inhibitory synaptic transmission of riluzole in rat spinal dorsal horn |
title | Anti-allodynic and promotive effect on inhibitory synaptic transmission of riluzole in rat spinal dorsal horn |
title_full | Anti-allodynic and promotive effect on inhibitory synaptic transmission of riluzole in rat spinal dorsal horn |
title_fullStr | Anti-allodynic and promotive effect on inhibitory synaptic transmission of riluzole in rat spinal dorsal horn |
title_full_unstemmed | Anti-allodynic and promotive effect on inhibitory synaptic transmission of riluzole in rat spinal dorsal horn |
title_short | Anti-allodynic and promotive effect on inhibitory synaptic transmission of riluzole in rat spinal dorsal horn |
title_sort | anti-allodynic and promotive effect on inhibitory synaptic transmission of riluzole in rat spinal dorsal horn |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8435917/ https://www.ncbi.nlm.nih.gov/pubmed/34541342 http://dx.doi.org/10.1016/j.bbrep.2021.101130 |
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