Using piggyBac transposon gene expression vectors to transfect Zscan5b gene into mouse pluripotent stem cells

We recently demonstrated that the zygotic gene Zscan5b is involved in maintaining the genomic integrity of pluripotent stem cells (PSCs) during mitosis and that Zscan5b gene expression can prevent chromosomal abnormalities. Here, we provide a detailed protocol for the transfection of mouse embryonic...

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Detalles Bibliográficos
Autores principales: Yamada, Mitsutoshi, Sugawara, Tohru, Usami, Shinju, Nakanishi, Rina, Akustu, Hidenori
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8436169/
https://www.ncbi.nlm.nih.gov/pubmed/34541557
http://dx.doi.org/10.1016/j.xpro.2021.100811
Descripción
Sumario:We recently demonstrated that the zygotic gene Zscan5b is involved in maintaining the genomic integrity of pluripotent stem cells (PSCs) during mitosis and that Zscan5b gene expression can prevent chromosomal abnormalities. Here, we provide a detailed protocol for the transfection of mouse embryonic stem cells (mESCs) and mouse-induced PSCs (miPSCs) with a Zscan5b constitutive expression plasmid, using the piggyBac transposon gene expression vector system. For complete details on the use and execution of this protocol, please refer to Ogawa et al. (2019).

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