Using piggyBac transposon gene expression vectors to transfect Zscan5b gene into mouse pluripotent stem cells

We recently demonstrated that the zygotic gene Zscan5b is involved in maintaining the genomic integrity of pluripotent stem cells (PSCs) during mitosis and that Zscan5b gene expression can prevent chromosomal abnormalities. Here, we provide a detailed protocol for the transfection of mouse embryonic...

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Autores principales: Yamada, Mitsutoshi, Sugawara, Tohru, Usami, Shinju, Nakanishi, Rina, Akustu, Hidenori
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8436169/
https://www.ncbi.nlm.nih.gov/pubmed/34541557
http://dx.doi.org/10.1016/j.xpro.2021.100811
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author Yamada, Mitsutoshi
Sugawara, Tohru
Usami, Shinju
Nakanishi, Rina
Akustu, Hidenori
author_facet Yamada, Mitsutoshi
Sugawara, Tohru
Usami, Shinju
Nakanishi, Rina
Akustu, Hidenori
author_sort Yamada, Mitsutoshi
collection PubMed
description We recently demonstrated that the zygotic gene Zscan5b is involved in maintaining the genomic integrity of pluripotent stem cells (PSCs) during mitosis and that Zscan5b gene expression can prevent chromosomal abnormalities. Here, we provide a detailed protocol for the transfection of mouse embryonic stem cells (mESCs) and mouse-induced PSCs (miPSCs) with a Zscan5b constitutive expression plasmid, using the piggyBac transposon gene expression vector system. For complete details on the use and execution of this protocol, please refer to Ogawa et al. (2019).
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spelling pubmed-84361692021-09-17 Using piggyBac transposon gene expression vectors to transfect Zscan5b gene into mouse pluripotent stem cells Yamada, Mitsutoshi Sugawara, Tohru Usami, Shinju Nakanishi, Rina Akustu, Hidenori STAR Protoc Protocol We recently demonstrated that the zygotic gene Zscan5b is involved in maintaining the genomic integrity of pluripotent stem cells (PSCs) during mitosis and that Zscan5b gene expression can prevent chromosomal abnormalities. Here, we provide a detailed protocol for the transfection of mouse embryonic stem cells (mESCs) and mouse-induced PSCs (miPSCs) with a Zscan5b constitutive expression plasmid, using the piggyBac transposon gene expression vector system. For complete details on the use and execution of this protocol, please refer to Ogawa et al. (2019). Elsevier 2021-09-11 /pmc/articles/PMC8436169/ /pubmed/34541557 http://dx.doi.org/10.1016/j.xpro.2021.100811 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Yamada, Mitsutoshi
Sugawara, Tohru
Usami, Shinju
Nakanishi, Rina
Akustu, Hidenori
Using piggyBac transposon gene expression vectors to transfect Zscan5b gene into mouse pluripotent stem cells
title Using piggyBac transposon gene expression vectors to transfect Zscan5b gene into mouse pluripotent stem cells
title_full Using piggyBac transposon gene expression vectors to transfect Zscan5b gene into mouse pluripotent stem cells
title_fullStr Using piggyBac transposon gene expression vectors to transfect Zscan5b gene into mouse pluripotent stem cells
title_full_unstemmed Using piggyBac transposon gene expression vectors to transfect Zscan5b gene into mouse pluripotent stem cells
title_short Using piggyBac transposon gene expression vectors to transfect Zscan5b gene into mouse pluripotent stem cells
title_sort using piggybac transposon gene expression vectors to transfect zscan5b gene into mouse pluripotent stem cells
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8436169/
https://www.ncbi.nlm.nih.gov/pubmed/34541557
http://dx.doi.org/10.1016/j.xpro.2021.100811
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