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Using piggyBac transposon gene expression vectors to transfect Zscan5b gene into mouse pluripotent stem cells
We recently demonstrated that the zygotic gene Zscan5b is involved in maintaining the genomic integrity of pluripotent stem cells (PSCs) during mitosis and that Zscan5b gene expression can prevent chromosomal abnormalities. Here, we provide a detailed protocol for the transfection of mouse embryonic...
| Autores principales: | , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2021
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8436169/ https://www.ncbi.nlm.nih.gov/pubmed/34541557 http://dx.doi.org/10.1016/j.xpro.2021.100811 |