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miR-486-5p suppresses gastric cancer cell growth and migration through downregulation of fibroblast growth factor 9
Non-coding RNAs serve essential roles in regulating mRNA and protein expression and dysregulation of non-coding RNAs participates in a variety of types of cancer. microRNAs (miRNAs/miRs), which are 21–24 nucleotides non-coding RNAs, have been shown to be important for the development of gastric canc...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8436225/ https://www.ncbi.nlm.nih.gov/pubmed/34490480 http://dx.doi.org/10.3892/mmr.2021.12411 |
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author | Wei, Weiwei Liu, Chunyu Yao, Rongrong Tan, Qingyun Wang, Qingdong Tian, Hao |
author_facet | Wei, Weiwei Liu, Chunyu Yao, Rongrong Tan, Qingyun Wang, Qingdong Tian, Hao |
author_sort | Wei, Weiwei |
collection | PubMed |
description | Non-coding RNAs serve essential roles in regulating mRNA and protein expression and dysregulation of non-coding RNAs participates in a variety of types of cancer. microRNAs (miRNAs/miRs), which are 21–24 nucleotides non-coding RNAs, have been shown to be important for the development of gastric cancer (GC). However, the role of miR-486-5p in GC remains to be elucidated. The present study found that miR-486-5p was downregulated in GC tissues. Comparing with gastric normal cells GES-1, GC cells, including MKN-45, AGS, HGC27 and MKN74, had reduced abundance of miR-486-5p transcript. CCK8 and colony formation assays demonstrated that GC cell growth and proliferation were enhanced by miR-486-5p inhibitors and were suppressed by miR-486-5p mimics. miR-486-5p also suppressed cell cycle process and migration and promoted apoptosis in GC cells, as verified by propidium iodide (PI) staining, Transwell assay and PI/Annexin V staining. miR-486-5p downregulated fibroblast growth factor 9 (FGF9) through combining to its 3′untranslated region. Overexpression of FGF9 accelerated the growth and proliferation of GC cells. The expression of miR-486-5p was negatively associated with FGF9 mRNA expression in GC samples. These results revealed that miR-486-5p was a tumor suppressor in GC. Downregulation of FGF9 contributed to the role of miR-486-5p in GC. |
format | Online Article Text |
id | pubmed-8436225 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-84362252021-09-17 miR-486-5p suppresses gastric cancer cell growth and migration through downregulation of fibroblast growth factor 9 Wei, Weiwei Liu, Chunyu Yao, Rongrong Tan, Qingyun Wang, Qingdong Tian, Hao Mol Med Rep Articles Non-coding RNAs serve essential roles in regulating mRNA and protein expression and dysregulation of non-coding RNAs participates in a variety of types of cancer. microRNAs (miRNAs/miRs), which are 21–24 nucleotides non-coding RNAs, have been shown to be important for the development of gastric cancer (GC). However, the role of miR-486-5p in GC remains to be elucidated. The present study found that miR-486-5p was downregulated in GC tissues. Comparing with gastric normal cells GES-1, GC cells, including MKN-45, AGS, HGC27 and MKN74, had reduced abundance of miR-486-5p transcript. CCK8 and colony formation assays demonstrated that GC cell growth and proliferation were enhanced by miR-486-5p inhibitors and were suppressed by miR-486-5p mimics. miR-486-5p also suppressed cell cycle process and migration and promoted apoptosis in GC cells, as verified by propidium iodide (PI) staining, Transwell assay and PI/Annexin V staining. miR-486-5p downregulated fibroblast growth factor 9 (FGF9) through combining to its 3′untranslated region. Overexpression of FGF9 accelerated the growth and proliferation of GC cells. The expression of miR-486-5p was negatively associated with FGF9 mRNA expression in GC samples. These results revealed that miR-486-5p was a tumor suppressor in GC. Downregulation of FGF9 contributed to the role of miR-486-5p in GC. D.A. Spandidos 2021-11 2021-09-03 /pmc/articles/PMC8436225/ /pubmed/34490480 http://dx.doi.org/10.3892/mmr.2021.12411 Text en Copyright: © Wei et al. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Wei, Weiwei Liu, Chunyu Yao, Rongrong Tan, Qingyun Wang, Qingdong Tian, Hao miR-486-5p suppresses gastric cancer cell growth and migration through downregulation of fibroblast growth factor 9 |
title | miR-486-5p suppresses gastric cancer cell growth and migration through downregulation of fibroblast growth factor 9 |
title_full | miR-486-5p suppresses gastric cancer cell growth and migration through downregulation of fibroblast growth factor 9 |
title_fullStr | miR-486-5p suppresses gastric cancer cell growth and migration through downregulation of fibroblast growth factor 9 |
title_full_unstemmed | miR-486-5p suppresses gastric cancer cell growth and migration through downregulation of fibroblast growth factor 9 |
title_short | miR-486-5p suppresses gastric cancer cell growth and migration through downregulation of fibroblast growth factor 9 |
title_sort | mir-486-5p suppresses gastric cancer cell growth and migration through downregulation of fibroblast growth factor 9 |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8436225/ https://www.ncbi.nlm.nih.gov/pubmed/34490480 http://dx.doi.org/10.3892/mmr.2021.12411 |
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