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EGFR and ERK activation resists flavonoid quercetin-induced anticancer activities in human cervical cancer cells in vitro

In the present study, due to the complex and numerous targets of Sarcandrae Herb (also known as Zhong Jie Feng), network pharmacology was performed to analyze its therapeutic effect on 2 cervical cancer cell lines, which could assist with the development of novel therapies. The results suggested tha...

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Autores principales: Chen, Xin, Xu, Pengli, Zhang, Huijun, Su, Xiaosan, Guo, Lihua, Zhou, Xuhong, Wang, Junliang, Huang, Peng, Zhang, Qingzhi, Sun, Ruifen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8436358/
https://www.ncbi.nlm.nih.gov/pubmed/34539858
http://dx.doi.org/10.3892/ol.2021.13015
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author Chen, Xin
Xu, Pengli
Zhang, Huijun
Su, Xiaosan
Guo, Lihua
Zhou, Xuhong
Wang, Junliang
Huang, Peng
Zhang, Qingzhi
Sun, Ruifen
author_facet Chen, Xin
Xu, Pengli
Zhang, Huijun
Su, Xiaosan
Guo, Lihua
Zhou, Xuhong
Wang, Junliang
Huang, Peng
Zhang, Qingzhi
Sun, Ruifen
author_sort Chen, Xin
collection PubMed
description In the present study, due to the complex and numerous targets of Sarcandrae Herb (also known as Zhong Jie Feng), network pharmacology was performed to analyze its therapeutic effect on 2 cervical cancer cell lines, which could assist with the development of novel therapies. The results suggested that the natural flavonoid quercetin (Que), the effective antitumor ingredient in SH, which is widely present in a variety of plants, may depend on the target, EGFR. Previous studies have shown that EGFR serves a crucial role in the occurrence and development of cervical cancer, but its downstream molecules and regulatory mechanisms remain unknown. The anti-cervical cancer cell properties of Que, which are present in ubiquitous plants, were examined in vitro to identify the association between Que and its underlying pathway using MTT assays, flow cytometry, western blot analysis and Transwell assays. It was found that Que reduced cervical cancer cell viability, promoted G(2)/M phase cell cycle arrest and cell apoptosis, as well as inhibited cell migration and invasion. The Tyr1068 phosphorylation site of EGFR and the corresponding ERK target were also examined and the 2 kinases were markedly activated by Que. Furthermore, the EGFR inhibitor, afatinib and the ERK inhibitor, U0126 blocked the increase of EGFR and ERK phosphorylation, and resulted in a notable enhancement of apoptosis and cell cycle arrest. Therefore, to the best of our knowledge, the current results provided the first evidence that EGFR and ERK activation induced by Que could resist Que-induced anticancer activities. On this basis, the present study determined the role of EGFR and the underlying signaling pathways involved in the anti-cervical cancer malignant behavior induced by Que and identified the negative regulatory association.
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spelling pubmed-84363582021-09-17 EGFR and ERK activation resists flavonoid quercetin-induced anticancer activities in human cervical cancer cells in vitro Chen, Xin Xu, Pengli Zhang, Huijun Su, Xiaosan Guo, Lihua Zhou, Xuhong Wang, Junliang Huang, Peng Zhang, Qingzhi Sun, Ruifen Oncol Lett Articles In the present study, due to the complex and numerous targets of Sarcandrae Herb (also known as Zhong Jie Feng), network pharmacology was performed to analyze its therapeutic effect on 2 cervical cancer cell lines, which could assist with the development of novel therapies. The results suggested that the natural flavonoid quercetin (Que), the effective antitumor ingredient in SH, which is widely present in a variety of plants, may depend on the target, EGFR. Previous studies have shown that EGFR serves a crucial role in the occurrence and development of cervical cancer, but its downstream molecules and regulatory mechanisms remain unknown. The anti-cervical cancer cell properties of Que, which are present in ubiquitous plants, were examined in vitro to identify the association between Que and its underlying pathway using MTT assays, flow cytometry, western blot analysis and Transwell assays. It was found that Que reduced cervical cancer cell viability, promoted G(2)/M phase cell cycle arrest and cell apoptosis, as well as inhibited cell migration and invasion. The Tyr1068 phosphorylation site of EGFR and the corresponding ERK target were also examined and the 2 kinases were markedly activated by Que. Furthermore, the EGFR inhibitor, afatinib and the ERK inhibitor, U0126 blocked the increase of EGFR and ERK phosphorylation, and resulted in a notable enhancement of apoptosis and cell cycle arrest. Therefore, to the best of our knowledge, the current results provided the first evidence that EGFR and ERK activation induced by Que could resist Que-induced anticancer activities. On this basis, the present study determined the role of EGFR and the underlying signaling pathways involved in the anti-cervical cancer malignant behavior induced by Que and identified the negative regulatory association. D.A. Spandidos 2021-11 2021-08-31 /pmc/articles/PMC8436358/ /pubmed/34539858 http://dx.doi.org/10.3892/ol.2021.13015 Text en Copyright: © Chen et al. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Chen, Xin
Xu, Pengli
Zhang, Huijun
Su, Xiaosan
Guo, Lihua
Zhou, Xuhong
Wang, Junliang
Huang, Peng
Zhang, Qingzhi
Sun, Ruifen
EGFR and ERK activation resists flavonoid quercetin-induced anticancer activities in human cervical cancer cells in vitro
title EGFR and ERK activation resists flavonoid quercetin-induced anticancer activities in human cervical cancer cells in vitro
title_full EGFR and ERK activation resists flavonoid quercetin-induced anticancer activities in human cervical cancer cells in vitro
title_fullStr EGFR and ERK activation resists flavonoid quercetin-induced anticancer activities in human cervical cancer cells in vitro
title_full_unstemmed EGFR and ERK activation resists flavonoid quercetin-induced anticancer activities in human cervical cancer cells in vitro
title_short EGFR and ERK activation resists flavonoid quercetin-induced anticancer activities in human cervical cancer cells in vitro
title_sort egfr and erk activation resists flavonoid quercetin-induced anticancer activities in human cervical cancer cells in vitro
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8436358/
https://www.ncbi.nlm.nih.gov/pubmed/34539858
http://dx.doi.org/10.3892/ol.2021.13015
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