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circRNA circ_POLA2 increases microRNA-31 methylation to promote endometrial cancer cell proliferation
Circular RNA (circRNA) circ_POLA2 is an oncogene in lung and cervical cancers. However, the role of circ_POLA2 in other types of cancer is unknown. The present study investigated the role of circ_POLA2 in endometrial cancer (EC). The mRNA expression levels of circ_POLA2 and microRNA (miR)-31 in EC a...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8436404/ https://www.ncbi.nlm.nih.gov/pubmed/34539866 http://dx.doi.org/10.3892/ol.2021.13023 |
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author | Fang, Xia Wang, Jinhua Chen, Lingying Zhang, Xiaochun |
author_facet | Fang, Xia Wang, Jinhua Chen, Lingying Zhang, Xiaochun |
author_sort | Fang, Xia |
collection | PubMed |
description | Circular RNA (circRNA) circ_POLA2 is an oncogene in lung and cervical cancers. However, the role of circ_POLA2 in other types of cancer is unknown. The present study investigated the role of circ_POLA2 in endometrial cancer (EC). The mRNA expression levels of circ_POLA2 and microRNA (miR)-31 in EC and paired adjacent normal tissues were analyzed using reverse transcription-quantitative (RT-qPCR). Overexpression of circ_POLA2 was achieved in the EC cell lines, and its effects on miR-31 mRNA expression level and methylation were evaluated using RT-qPCR and methylation-specific PCR (MSP), respectively. Cell proliferation was assessed using a Cell Counting Kit-8 assay. The results indicated that circ_POLA2 was highly expressed in EC tissue and inversely correlated with miR-31 mRNA expression level. MSP analysis showed that circ_POLA2 overexpression increased miR-31 methylation and RT-qPCR analysis showed that circ_POLA2 overexpression decreased miR-31 mRNA expression level. Furthermore, circ_POLA2 overexpression also increased EC cell proliferation, while miR-31 overexpression decreased cell proliferation. Finally, circ_POLA2 overexpression reduced the effects of miR-31 overexpression. In conclusion, circ_POLA2 may increase miR-31 methylation of miR-31 in EC cells to promote cancer cell proliferation. |
format | Online Article Text |
id | pubmed-8436404 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-84364042021-09-17 circRNA circ_POLA2 increases microRNA-31 methylation to promote endometrial cancer cell proliferation Fang, Xia Wang, Jinhua Chen, Lingying Zhang, Xiaochun Oncol Lett Articles Circular RNA (circRNA) circ_POLA2 is an oncogene in lung and cervical cancers. However, the role of circ_POLA2 in other types of cancer is unknown. The present study investigated the role of circ_POLA2 in endometrial cancer (EC). The mRNA expression levels of circ_POLA2 and microRNA (miR)-31 in EC and paired adjacent normal tissues were analyzed using reverse transcription-quantitative (RT-qPCR). Overexpression of circ_POLA2 was achieved in the EC cell lines, and its effects on miR-31 mRNA expression level and methylation were evaluated using RT-qPCR and methylation-specific PCR (MSP), respectively. Cell proliferation was assessed using a Cell Counting Kit-8 assay. The results indicated that circ_POLA2 was highly expressed in EC tissue and inversely correlated with miR-31 mRNA expression level. MSP analysis showed that circ_POLA2 overexpression increased miR-31 methylation and RT-qPCR analysis showed that circ_POLA2 overexpression decreased miR-31 mRNA expression level. Furthermore, circ_POLA2 overexpression also increased EC cell proliferation, while miR-31 overexpression decreased cell proliferation. Finally, circ_POLA2 overexpression reduced the effects of miR-31 overexpression. In conclusion, circ_POLA2 may increase miR-31 methylation of miR-31 in EC cells to promote cancer cell proliferation. D.A. Spandidos 2021-11 2021-09-06 /pmc/articles/PMC8436404/ /pubmed/34539866 http://dx.doi.org/10.3892/ol.2021.13023 Text en Copyright: © Fang et al. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Fang, Xia Wang, Jinhua Chen, Lingying Zhang, Xiaochun circRNA circ_POLA2 increases microRNA-31 methylation to promote endometrial cancer cell proliferation |
title | circRNA circ_POLA2 increases microRNA-31 methylation to promote endometrial cancer cell proliferation |
title_full | circRNA circ_POLA2 increases microRNA-31 methylation to promote endometrial cancer cell proliferation |
title_fullStr | circRNA circ_POLA2 increases microRNA-31 methylation to promote endometrial cancer cell proliferation |
title_full_unstemmed | circRNA circ_POLA2 increases microRNA-31 methylation to promote endometrial cancer cell proliferation |
title_short | circRNA circ_POLA2 increases microRNA-31 methylation to promote endometrial cancer cell proliferation |
title_sort | circrna circ_pola2 increases microrna-31 methylation to promote endometrial cancer cell proliferation |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8436404/ https://www.ncbi.nlm.nih.gov/pubmed/34539866 http://dx.doi.org/10.3892/ol.2021.13023 |
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