Cargando…
High extracellular phosphate increases platelet polyphosphate content
Platelet-derived extracellular polyphosphate (PolyP) is a major mediator of thrombosis. PolyP is a linear chain of inorganic phosphate (P(i)) and is stored in platelet dense granules. P(i) enters cells from the extracellular fluid through phosphate transporters and may be stored as PolyP. Here we sh...
Autores principales: | , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8437092/ https://www.ncbi.nlm.nih.gov/pubmed/32892685 http://dx.doi.org/10.1080/09537104.2020.1817358 |
_version_ | 1783752107167318016 |
---|---|
author | Abbasian, Nima Harper, Matthew T. |
author_facet | Abbasian, Nima Harper, Matthew T. |
author_sort | Abbasian, Nima |
collection | PubMed |
description | Platelet-derived extracellular polyphosphate (PolyP) is a major mediator of thrombosis. PolyP is a linear chain of inorganic phosphate (P(i)) and is stored in platelet dense granules. P(i) enters cells from the extracellular fluid through phosphate transporters and may be stored as PolyP. Here we show that high extracellular P(i) concentration in vitro increases platelet PolyP content, in a manner dependent on phosphate transporters, IP6K and V-type ATPases. The increased PolyP also enhanced PolyP-dependent coagulation in platelet-rich plasma. These data suggest a mechanistic link between hyperphosphatemia, PolyP and enhanced coagulation, which may be important in pathologies such as chronic kidney disease. |
format | Online Article Text |
id | pubmed-8437092 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-84370922021-09-14 High extracellular phosphate increases platelet polyphosphate content Abbasian, Nima Harper, Matthew T. Platelets Brief Report Platelet-derived extracellular polyphosphate (PolyP) is a major mediator of thrombosis. PolyP is a linear chain of inorganic phosphate (P(i)) and is stored in platelet dense granules. P(i) enters cells from the extracellular fluid through phosphate transporters and may be stored as PolyP. Here we show that high extracellular P(i) concentration in vitro increases platelet PolyP content, in a manner dependent on phosphate transporters, IP6K and V-type ATPases. The increased PolyP also enhanced PolyP-dependent coagulation in platelet-rich plasma. These data suggest a mechanistic link between hyperphosphatemia, PolyP and enhanced coagulation, which may be important in pathologies such as chronic kidney disease. Taylor & Francis 2020-09-06 /pmc/articles/PMC8437092/ /pubmed/32892685 http://dx.doi.org/10.1080/09537104.2020.1817358 Text en © 2020 The Author(s). Published with license by Taylor & Francis Group, LLC. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Brief Report Abbasian, Nima Harper, Matthew T. High extracellular phosphate increases platelet polyphosphate content |
title | High extracellular phosphate increases platelet polyphosphate content |
title_full | High extracellular phosphate increases platelet polyphosphate content |
title_fullStr | High extracellular phosphate increases platelet polyphosphate content |
title_full_unstemmed | High extracellular phosphate increases platelet polyphosphate content |
title_short | High extracellular phosphate increases platelet polyphosphate content |
title_sort | high extracellular phosphate increases platelet polyphosphate content |
topic | Brief Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8437092/ https://www.ncbi.nlm.nih.gov/pubmed/32892685 http://dx.doi.org/10.1080/09537104.2020.1817358 |
work_keys_str_mv | AT abbasiannima highextracellularphosphateincreasesplateletpolyphosphatecontent AT harpermatthewt highextracellularphosphateincreasesplateletpolyphosphatecontent |