Cargando…

Tuberculosis seroprevalence and comparison of hematology and biochemistry parameters between seropositive and seronegative captive Asian elephants of Nepal

We conducted a tuberculosis (TB) serosurveillance program of captive elephants in Nepal and compared hematology and biochemistry parameters between seropositive and seronegative elephants. A total of 153 elephants (male=20, female=133) from four national parks were tested for TB using the ElephantTB...

Descripción completa

Detalles Bibliográficos
Autores principales: THAPA, Jeewan, MIKOTA, Susan K., GAIRHE, Kamal Prasad, PAUDEL, Sarad, SINGH, Dinesh Kumar, DHAKAL, Ishwari Prasad, NAKAJIMA, Chie, SUZUKI, Yasuhiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8437712/
https://www.ncbi.nlm.nih.gov/pubmed/34108338
http://dx.doi.org/10.1292/jvms.21-0113
_version_ 1783752212451688448
author THAPA, Jeewan
MIKOTA, Susan K.
GAIRHE, Kamal Prasad
PAUDEL, Sarad
SINGH, Dinesh Kumar
DHAKAL, Ishwari Prasad
NAKAJIMA, Chie
SUZUKI, Yasuhiko
author_facet THAPA, Jeewan
MIKOTA, Susan K.
GAIRHE, Kamal Prasad
PAUDEL, Sarad
SINGH, Dinesh Kumar
DHAKAL, Ishwari Prasad
NAKAJIMA, Chie
SUZUKI, Yasuhiko
author_sort THAPA, Jeewan
collection PubMed
description We conducted a tuberculosis (TB) serosurveillance program of captive elephants in Nepal and compared hematology and biochemistry parameters between seropositive and seronegative elephants. A total of 153 elephants (male=20, female=133) from four national parks were tested for TB using the ElephantTB STAT-PAK(®) Assay (ChemBio Diagnostic Systems, Inc., Medford, NY, USA). The mean reported age for 138 elephants was 38.5 years (range 2–71 years). Seroprevalence for TB was 21.56% (33/153). The majority of seropositive elephants were female (n=30) and from Chitwan National Park (n=29). The occurrence of TB seropositive cases in other more remote national parks suggests TB may be widespread among the captive elephant population of Nepal. Hematology and biochemistry analyses were performed on 13 and 22 seropositive elephants, respectively and, nine elephants from a seronegative TB herd for comparison. Hematology parameters (hemoglobin, packed cell volume, platelet, white blood cells, and erythrocyte sedimentation rate) were comparable between the two groups. Total protein, globulin, and lactate dehydrogenase were significantly higher in seronegative elephants, and bilirubin was significantly higher in seropositive elephants whereas blood urea nitrogen, creatinine, glutamic oxaloacetic transaminase/aspartate aminotransferase (GOT/AST), glutamic pyruvic transaminase/alanine aminotransferase (GPT/ALT), gamma glutamyl transferase (GT), and albumin were not significantly different. The range of biochemical parameters that were significantly different between seropositive and seronegative elephants had narrow ranges. Thus, the potential of these parameters as a direct biomarker for TB diagnosis is limited based on the findings in this study. We recommend including blood parameters in future TB surveillance studies.
format Online
Article
Text
id pubmed-8437712
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher The Japanese Society of Veterinary Science
record_format MEDLINE/PubMed
spelling pubmed-84377122021-09-17 Tuberculosis seroprevalence and comparison of hematology and biochemistry parameters between seropositive and seronegative captive Asian elephants of Nepal THAPA, Jeewan MIKOTA, Susan K. GAIRHE, Kamal Prasad PAUDEL, Sarad SINGH, Dinesh Kumar DHAKAL, Ishwari Prasad NAKAJIMA, Chie SUZUKI, Yasuhiko J Vet Med Sci Wildlife Science We conducted a tuberculosis (TB) serosurveillance program of captive elephants in Nepal and compared hematology and biochemistry parameters between seropositive and seronegative elephants. A total of 153 elephants (male=20, female=133) from four national parks were tested for TB using the ElephantTB STAT-PAK(®) Assay (ChemBio Diagnostic Systems, Inc., Medford, NY, USA). The mean reported age for 138 elephants was 38.5 years (range 2–71 years). Seroprevalence for TB was 21.56% (33/153). The majority of seropositive elephants were female (n=30) and from Chitwan National Park (n=29). The occurrence of TB seropositive cases in other more remote national parks suggests TB may be widespread among the captive elephant population of Nepal. Hematology and biochemistry analyses were performed on 13 and 22 seropositive elephants, respectively and, nine elephants from a seronegative TB herd for comparison. Hematology parameters (hemoglobin, packed cell volume, platelet, white blood cells, and erythrocyte sedimentation rate) were comparable between the two groups. Total protein, globulin, and lactate dehydrogenase were significantly higher in seronegative elephants, and bilirubin was significantly higher in seropositive elephants whereas blood urea nitrogen, creatinine, glutamic oxaloacetic transaminase/aspartate aminotransferase (GOT/AST), glutamic pyruvic transaminase/alanine aminotransferase (GPT/ALT), gamma glutamyl transferase (GT), and albumin were not significantly different. The range of biochemical parameters that were significantly different between seropositive and seronegative elephants had narrow ranges. Thus, the potential of these parameters as a direct biomarker for TB diagnosis is limited based on the findings in this study. We recommend including blood parameters in future TB surveillance studies. The Japanese Society of Veterinary Science 2021-06-09 2021-08 /pmc/articles/PMC8437712/ /pubmed/34108338 http://dx.doi.org/10.1292/jvms.21-0113 Text en ©2021 The Japanese Society of Veterinary Science https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)
spellingShingle Wildlife Science
THAPA, Jeewan
MIKOTA, Susan K.
GAIRHE, Kamal Prasad
PAUDEL, Sarad
SINGH, Dinesh Kumar
DHAKAL, Ishwari Prasad
NAKAJIMA, Chie
SUZUKI, Yasuhiko
Tuberculosis seroprevalence and comparison of hematology and biochemistry parameters between seropositive and seronegative captive Asian elephants of Nepal
title Tuberculosis seroprevalence and comparison of hematology and biochemistry parameters between seropositive and seronegative captive Asian elephants of Nepal
title_full Tuberculosis seroprevalence and comparison of hematology and biochemistry parameters between seropositive and seronegative captive Asian elephants of Nepal
title_fullStr Tuberculosis seroprevalence and comparison of hematology and biochemistry parameters between seropositive and seronegative captive Asian elephants of Nepal
title_full_unstemmed Tuberculosis seroprevalence and comparison of hematology and biochemistry parameters between seropositive and seronegative captive Asian elephants of Nepal
title_short Tuberculosis seroprevalence and comparison of hematology and biochemistry parameters between seropositive and seronegative captive Asian elephants of Nepal
title_sort tuberculosis seroprevalence and comparison of hematology and biochemistry parameters between seropositive and seronegative captive asian elephants of nepal
topic Wildlife Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8437712/
https://www.ncbi.nlm.nih.gov/pubmed/34108338
http://dx.doi.org/10.1292/jvms.21-0113
work_keys_str_mv AT thapajeewan tuberculosisseroprevalenceandcomparisonofhematologyandbiochemistryparametersbetweenseropositiveandseronegativecaptiveasianelephantsofnepal
AT mikotasusank tuberculosisseroprevalenceandcomparisonofhematologyandbiochemistryparametersbetweenseropositiveandseronegativecaptiveasianelephantsofnepal
AT gairhekamalprasad tuberculosisseroprevalenceandcomparisonofhematologyandbiochemistryparametersbetweenseropositiveandseronegativecaptiveasianelephantsofnepal
AT paudelsarad tuberculosisseroprevalenceandcomparisonofhematologyandbiochemistryparametersbetweenseropositiveandseronegativecaptiveasianelephantsofnepal
AT singhdineshkumar tuberculosisseroprevalenceandcomparisonofhematologyandbiochemistryparametersbetweenseropositiveandseronegativecaptiveasianelephantsofnepal
AT dhakalishwariprasad tuberculosisseroprevalenceandcomparisonofhematologyandbiochemistryparametersbetweenseropositiveandseronegativecaptiveasianelephantsofnepal
AT nakajimachie tuberculosisseroprevalenceandcomparisonofhematologyandbiochemistryparametersbetweenseropositiveandseronegativecaptiveasianelephantsofnepal
AT suzukiyasuhiko tuberculosisseroprevalenceandcomparisonofhematologyandbiochemistryparametersbetweenseropositiveandseronegativecaptiveasianelephantsofnepal